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Protein Purification : Principles and Practice /

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This updated edition of a widely accepted textbook now also includes discussions of the important techniques of high performance liquid chromatography and dye-ligand chromatography. The book is a practical guide on how to get started in planning a protein isolation and provides enough information to...

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Bibliographic Details
Main Author: Scopes, Robert K.
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: New York, NY : Springer New York, 1987.
Edition:Second edition.
Series:Springer advanced texts in chemistry.
Subjects:
Life sciences.
Biochemistry.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • The Enzyme Purification Laboratory
  • Making an Extract
  • Separation by Precipitation
  • Separation by Adsorption I. General Principles
  • Separation by Adsorption II. Specific Adsorbents
  • Separation in Solution
  • Optimization of Procedures; Recombinant Protein Techniques
  • Maintenance of Active Enzymes
  • Measurement of Enzyme Activity
  • Analysis for Purity: Crystallization
  • Addition of Precipitants
  • Solutions for Measuring Protein Concentration
  • Appendices
  • References
  • Index.