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Protein purification : priciples and practice /

The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods ef...

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Bibliographic Details
Main Author: Scopes, Robert K.
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: New York : Springer-Verlag, [1994]
Edition:3rd ed.
Series:Springer advanced texts in chemistry.
Subjects:
Proteins > Purification.
Proteins > isolation & purification.
Protéines > Purification.
Eiwitten.
Zuiveringstechnieken.
Extractie (scheikunde)
Scheidingstechnieken.
Trennverfahren.
Proteine.
Proteins > Chemical analysis
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Protein purification laboratory
  • Making an extract
  • Analysis: measurement of protein and enzyme activity
  • Separation by precipitation
  • Separation by adsorption I: general aspects
  • Separation by adsorption II: ion exchangers and nonspecific adsorbents
  • Separation by adsorption: affinity techniques
  • Separation in solution
  • Purification of special types of proteins
  • Small-scale and large-scale procedures
  • Analysis for purity
  • Optimization of procedures: final steps
  • Precipitation tables
  • Solutions for measuring protein concentration
  • Buffers for use in protein chemistry
  • Chromatographic materials.