Animal Models of Disease Part B

This volume, part of the 'Methods in Cell Biology' series, focuses on animal models of disease, providing a comprehensive overview of methodologies used in research to simulate human diseases in animals. Edited by experts from various institutions, the book explores topics such as the use...

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Bibliographic Details
Corporate Author:	ScienceDirect (Online service)
Format:	eBook
Language:	English
Published:	Amsterdam : Academic Press, 2024.
Series:	Methods in cell biology ; v. 197.
Subjects:	Animal models in research. Pathology, Experimental. Electronic books.
Online Access:	Connect to the full text of this electronic book

Table of Contents:

Intro
Animal Models of Disease
Part B
Copyright
Contents
Contributors
Animal models of disease: Achievements and challenges
Acknowledgments
Competing interests
References
Chapter One: Using C. elegans as a model for neurodegenerative diseases: Methodology and evaluation
1. Introduction
2. Methods and strategies to generate C. elegans neurodegenerative models
3. Methods and strategies to validate C. elegans neurodegenerative models
3.1. C. elegans lifetime cycle synchronization
3.1.1. Purpose
3.1.2. Materials
3.1.3. Methods
3.2. Survival assay
3.2.1. Purpose
3.2.2. Materials
3.2.3. Methods
3.2.4. Application of survival assays to validate neurodegenerative models
3.3. Fertility assay
3.3.1. Purpose
3.3.2. Materials
3.3.3. Methods
3.3.4. Application of fertility assays to validate neurodegenerative models
3.4. Mobility assay
3.4.1. Purpose
3.4.2. Materials
3.4.3. Methods
3.4.4. Video Analysis with Fiji-ImageJ software
3.4.5. Application of mobility assays to validate neurodegenerative models
3.5. Electropharyngeogram assays
3.5.1. Purpose
3.5.2. Materials
3.5.3. Methods
3.5.4. Application of electropharyngeogram assays to validate neurodegenerative models
3.6. Confocal imaging
3.6.1. Purpose
3.6.2. Materials
3.6.3. Methods
3.6.4. Application of mitochondria confocal imaging assays to validate neurodegenerative models
3.7. C. elegans RNA extraction for qRT-PCR and RT-PCR
3.7.1. Purpose
3.7.2. Materials (see Note 13)
3.7.3. Methods
3.7.4. Application of qRT-PCR and RT-PCR to validate neurodegenerative models
3.8. Defecation rate measurements
3.8.1. Purpose
3.8.2. Materials
3.8.3. Methods
3.8.4. Application of rate of defecation measurement assays to validate the neurodegenerative models.
4. Discussion
5. Notes
References
Chapter Two: Animal model of multiple sclerosis: Experimental autoimmune encephalomyelitis
1. Introduction
2. Models for multiple sclerosis
2.1. Virus-induced chronic demyelinating disease
2.2. Toxin-induced demyelination
2.3. Experimental autoimmune encephalomyelitis
3. Spontaneous experimental autoimmune encephalomyelitis
4. Induced experimental autoimmune encephalomyelitis
5. EAE animal model
5.1. Primates
5.2. Rodents
6. EAE model: Advances in multiple sclerosis
6.1. Immune and inflammatory response
6.2. Oxidative stress
6.3. The role of the microbiota
6.4. Therapies and vaccines
7. In vitro models
8. Conclusions
References
Chapter Three: A new procedure to induce aortic aneurysms in mice
1. Introduction
2. Materials
2.1. Fungible material and reagents
2.2. Softwares and equipments
3. Experimental procedure
3.1. Tamoxifen administration
3.2. Genotyping
3.3. Angiotensin II infusion
3.4. Aortic ultrasound in vivo imaging
4. Conclusions remark
5. Notes
Acknowledgments
References
Chapter Four: Spinal nerve ligation: An experimental model to study neuropathic pain in rats and mice
1. Introduction
2. Materials
2.1. Animals
2.2. Drugs
2.3. Supplies and equipment
3. Methods: Surgical procedure
3.1. Rats
3.2. Mice
3.3. After the nerve ligation in rats and mice
4. Nociceptive behavior tests
4.1. Evoked stimulus nociceptive behavior
4.1.1. Tactile allodynia
4.1.2. Muscle hyperalgesia
4.1.3. Hargreaves test
4.2. Spontaneous nociceptive behavior in neuropathic animals
5. Results
5.1. Spinal nerve ligation induces evoked stimulus nociceptive behaviors in rats and mice
5.2. Spinal nerve ligation induces non-evoked stimulus nociceptive behavior in rats and mice.