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Radiation oncology and radiotherapy. Part B /

Radiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics including CT-assisted focal irradiation of tumors in mice, Methods to preserve correct dosimetry in s...

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Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Sato, Ai (Editor), Kraynak, Jeffrey (Editor), Marciscano, Ariel E. (Editor), Galluzz, Lorenzo (Editor)
Format: eBook
Language:English
Published: London, England : Academic Press, [2023]
Edition:First edition.
Series:Methods in cell biology ; volume 174.
Subjects:
Cancer > Radiotherapy.
Radiotherapy.
Radiotherapy
Neoplasms > radiotherapy
Radiation Oncology
Cancer > Radiothérapie.
Radiothérapie.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Cover
  • Series Editor
  • Radiation Oncology and Radiotherapy
  • Copyright
  • Contents
  • Contributors
  • Radiation therapy: An old dog learning new tricks
  • Acknowledgments
  • Conflict of interest
  • References
  • 1 Flow cytometry-assisted quantification of immune cells infiltrating irradiated tumors in mice
  • 1 Introduction
  • 2 Materials
  • 3 Methods
  • 4 FACS analysis and TIL quantification
  • 5 Timing of the sampling
  • 6 Notes
  • 7 Concluding remarks
  • Disclosures
  • Acknowledgements
  • References
  • 2 Cytofluorometric characterization of the myeloid compartment of irradiated mouse tumors
  • 1 Introduction
  • 2 Materials
  • 3 Cell culture
  • 4 Oral tumor implantation
  • 5 In vivo imaging
  • 6 Irradiation
  • 7 Flow cytometry analysis
  • 8 Concluding remarks
  • 9 Notes
  • Disclosures
  • Acknowledgments
  • References
  • 3 Mass cytometry to characterize the immune lung cancer microenvironment
  • 1 Introduction
  • 1.1 Multiparametric single-cell technologies to uncover the tumor microenvironment
  • 1.2 Mass cytometry or CyTOF (Cytometry by Time of Flight)
  • 1.3 Application of single-cell flow cytometry approaches to the study of solid tumors
  • 2 Materials
  • 2.1 Biological samples (See N1)
  • 2.2 Common disposables and equipment
  • 2.3 Common reagents
  • 2.4 Reagent setup (see N4)
  • 2.4.1 Solutions
  • 2.5 Antibody cocktail (see N6)
  • 3 Methods
  • 3.1 Sample collection
  • 3.2 Sample dissociation
  • 3.3 Sample staining (see N9)
  • 3.4 Sample acquisition
  • 4 Notes
  • 5 Concluding remarks
  • Acknowledgments
  • References
  • 4 Characterization of the immune infiltrate in mouse tissue by multiplex immunofluorescence
  • 1 Introduction
  • 2 Multiplex immunofluorescence staining
  • 3 Materials
  • 4 Methods
  • 4.1 Panel design considerations
  • 5 Multiplex imaging
  • 5.1 Image processing
  • 5.2 Cell segmentation
  • 5.3 Immunophenotyping analysis.
  • 5.4 Regional and neighboring analysis using k-nearest neighbors (kNN) methods
  • 6 Conclusion
  • Acknowledgements
  • References
  • 5 Ex vivo analysis of radiation effects on tumor infiltrating immune cells using tumor explants
  • 1 Introduction
  • 2 Materials
  • 2.1 Common disposables
  • 2.2 Common reagents
  • 2.3 Common equipment
  • 3 Cancer cell culture and in vivo tumor establishment
  • 4 Tumor explant processing
  • 5 Cell surface staining and viability determination
  • 6 Flow cytometry: Running samples and analysis protocol
  • 7 Notes
  • Acknowledgements
  • References
  • 6 Assessment of immunological memory formation in vivo
  • 1 Introduction
  • 2 Material
  • 2.1 Disposables
  • 2.2 Equipment
  • 2.3 Reagents
  • 2.4 Biological material
  • 3 Method
  • 3.1 Cell culture and preparation of cell suspension for tumor induction
  • 3.2 Subcutaneous injection of murine tumor cell (cell line A)
  • 3.3 Tumor growth and treatment
  • 3.4 Preparation of cell suspensions for tumor re-challenge
  • 3.5 Subcutaneous injection of murine tumor cells (cell line A and B)
  • 4 Notes
  • 5 Concluding remarks
  • References
  • 7 MorphoMacro for in vivo and ex vivo quantitative morphometric analysis of microglia
  • 1 Introduction
  • 2 Materials and methods
  • 3 Expected results
  • 4 Notes
  • 4.1 ImageJ plugins
  • 4.2 Description of morphological parameters
  • 4.3 Tissue preparation and image acquisition protocols
  • 4.4 Protocol of short-term ex vivo culture
  • 5 Concluding remarks
  • Funding
  • Acknowledgments
  • Appendix A Supporting information
  • References
  • 8 Quantitative assessment of mitophagy in irradiated cancer cells
  • 1 Introduction
  • 2 Materials
  • 2.1 Common disposables
  • 2.2 Cells and reagents
  • 2.3 Equipment
  • 2.4 Software
  • 3 Methods
  • 3.1 Generation of TS/A cells stably expressing mt-mKeima (see Note 20)
  • 3.1.1 HEK293FT cell culture.