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Advances in applied microbiology. Vol. 118 /

Advances in Applied Microbiology, Volume 118 continues the comprehensive reach of this widely read and authoritative review source in microbiology.Users will find invaluable references and information on a variety of areas relating to the topics of microbiology.- Contains contributions from leading...

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Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Gadd, Geoffrey M. (Editor), Sariaslani, Sima (Editor)
Format: eBook
Language:English
Published: Cambridge, MA : Academic Press, [2022]
Edition:First edition.
Series:Advances in applied microbiology. v. 118.
Subjects:
Microbiology.
Industrial microbiology.
Microbiologie.
Microbiologie industrielle.
microbiology.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Chapter 1: CRISPR/Cas genome editing systems in thermophiles: Current status, associated challenges, and future perspectives
  • 1. Introduction
  • 2. Genetic engineering in thermophilic organisms
  • 2.1. Selection marker in thermophiles
  • 2.2. Strategies for the transformation of thermophiles
  • 2.3. Shuttle vectors for thermophiles
  • 2.4. Advance of gene deletion and integration system in thermophiles
  • 3. CRISPR tool kit in thermopholes
  • 3.1. Development and application of endogenous type I and III CRISPR/Cas based technologies in thermophiles
  • 3.2. Development and application of type II CRISPR/Cas9 based technologies in thermophiles
  • 3.2.1. SpCas9 mediated genome editing in moderate thermophiles
  • 3.2.2. Characterization of thermostable Cas9
  • 3.2.3. ThermoCas9 mediated genome editing in B. smithii
  • 3.2.4. GeoCas9 mediated genome editing in C. thermocellum
  • 3.2.5. GeoCas9 mediated genome editing in T. ethanolicus
  • 3.2.6. CaldoCas9 mediated genome editing in T. thermophilus
  • 3.3. Development and application of CRISPR interference technologies in thermophiles
  • 4. Associated challenges and future perspectives
  • 4.1. A challenge in the introduction of DNA into thermophilic host cells
  • 4.2. A challenge in a controllable expression of thermostable Cas9 and sgRNA
  • 4.3. A challenge in the homology directed repair
  • 4.4. Construction of thermophilic microbial cell factory
  • 5. Conclusion
  • Acknowledgments
  • References
  • Chapter 2: Microbial mercury transformations: Molecules, functions and organisms
  • 1. Introduction
  • 2. Mercury methylation
  • 2.1. Hg methylation mechanisms
  • 2.2. Geochemical factors influencing Hg methylation
  • 2.2.1. SO4
  • 2.2.2. Natural organic matter
  • 2.2.3. Iron (Fe)
  • 2.2.4. Inorganic nutrients
  • 2.3. Previously-recognized and newly-discovered Hg methylating microbes
  • 2.3.1. Mercury methylating sulfate reducing bacteria (SRB)
  • 2.3.2. Mercury methylating iron reducing bacteria (FeRB)
  • 2.3.3. Mercury methylating methanogens
  • 2.3.4. Mercury methylating syntrophs
  • 2.3.5. Other novel species of mercury methylating prokaryotes
  • 2.4. Evolutionary and environmental implication of putative hgcAB genes
  • 3. Demethylation
  • 3.1. Reductive and oxidative biotic demethylation processes
  • 3.2. Reductive demethylation, or mer-dependent degradation to Hg(0) and CH4
  • 3.2.1. Mechanism of MerB activity
  • 3.2.2. Diversity, evolution, and distribution of MerB and microbes that reductively demethylate MeHg
  • 3.3. Oxidative demethylation
  • 3.4. Methanotrophic demethylation
  • 4. Redox transformations of inorganic mercury
  • 4.1. Inorganic mercury reduction
  • 4.2. Inorganic Hg oxidation
  • Acknowledgments
  • References
  • Chapter 3: Microbial community signatures for estimation of postmortem time intervals
  • 1. Introduction
  • 2. Signature microbes and their distribution in the human body
  • 2.1. Skin microbiome
  • 2.2. Oral microbiome
  • 2.3. Microbiome of respiratory tract
  • 2.4. Urinary system´s microbiome
  • 2.5. Microbiome of the human genital system
  • 2.6. Digestive system microbiome
  • 2.7. Other internal organs
  • 3. Molecular insight of change in human microenvironment postmortem
  • 4. Stages of decomposition
  • 5. Changes in signature microflora postmortem
  • 6. Techniques to study postmortem signature microflora
  • 6.1. Extraction of DNA from challenging samples
  • 6.2. Availability of group-specific primers
  • 6.3. Metagenomics
  • 6.4. Whole-genome sequencing
  • 6.5. Modeling techniques
  • 7. Conclusion
  • References.