Cover Image

Apobec Enzymes.

This book provides a comprehensive exploration of RNA editing, focusing on methodologies, techniques, and assays related to APOBEC enzymes. It includes detailed protocols for fluorescent shift assays, RNA sequencing, purification of APOBEC proteins, and in vitro deamination assays. The book also dis...

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Bibliographic Details
Main Author: Papavasiliou, F. Nina
Corporate Author: ScienceDirect (Online service)
Other Authors: Pecori, Riccardo
Format: eBook
Language:English
Published: Chantilly : Elsevier Science & Technology, 2025.
Edition:1st ed.
Series:Methods in Enzymology Series.
Subjects:
RNA editing.
Mutagenesis.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Cover image
  • Title page
  • Table of Contents
  • Series Page
  • Copyright
  • Contributors
  • Preface
  • Chapter One: Fluorescent shift assay for APOBEC-mediated RNA editing
  • Abstract
  • 1 Introduction
  • 2 Key materials
  • 3 Protocol
  • 4 Discussion and conclusion
  • 5 Notes
  • Acknowledgements
  • References
  • Chapter Two: Low-error RNA sequencing techniques for detecting RNA editing by APOBECs: Circular RNAseq assay and safe-sequencing system (SSS)
  • Abstract
  • 1 Introduction
  • 2 Circular-sequencing assay
  • 3 Materials and reagents
  • 4 Methods
  • 5 Circular RNAseq assay
  • 6 Materials and reagents
  • 7 Methods
  • 8 Conclusions
  • Acknowledgements
  • References
  • Chapter Three: Purification of enzymatically active APOBEC proteins from an insect cell expression system
  • Abstract