ADARs.
This volume focuses on methods and advancements in the study of Adenosine Deaminases Acting on RNA (ADARs), enzymes critical for RNA editing processes. It provides detailed protocols and methodologies for structural and functional analyses, purification techniques, and RNA editing applications in va...
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| Format: | eBook |
| Language: | English |
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Chantilly :
Elsevier Science & Technology,
2025.
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| Edition: | 1st ed. |
| Series: | Methods in Enzymology Series.
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| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Front Cover
- Series Page
- Methods in Enzymology
- Copyright
- Contents
- Contributors
- Preface
- Chapter One: Leveraging Saccharomyces cerevisiae for ADAR research: From high-yield purification to high-throughput screening and therapeutic applications
- 1 Introduction
- 2 High-yield purification of ADARs from Saccharomyces cerevisiae for advanced structural and functional studies
- 3 High-throughput screening in yeast promotes ADAR structure-function relationships and substrate preferences
- 4 Yeast-based screening system for optimizing guide RNA sequences that promote the RNA editing of disease-causing mutations
- 5 Exogenous expression of heterologous ADARs in yeast
- References
- Chapter Two: Structural analysis of human ADAR2-RNA complexes by X-ray crystallography
- 1 Introduction
- 2 Synthesis and purification of RNAs
- 2.1 Equipment
- 2.2 Materials
- 2.3 Synthesis and purification procedure
- 3 hADAR2 overexpression and purification for crystallization
- 3.1 Equipment
- 3.2 Materials
- 3.3 Large scale growth of BCY123 cells expressing hADAR2
- 3.4 First Ni-NTA purification of hADAR2
- 3.5 His-tag cleavage of hADAR2 via Tobacco Etch Virus (TEV) protease
- 4 TEV overexpression, purification, and storage
- 4.1 Equipment
- 4.2 Materials
- 4.3 Expression of TEV protocol