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|a TXAM
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|a Beal, Peter.
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| 245 |
1 |
0 |
|a ADARs.
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| 250 |
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|a 1st ed.
|
| 264 |
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1 |
|a Chantilly :
|b Elsevier Science & Technology,
|c 2025.
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4 |
|c ©2025.
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| 300 |
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|a Methods in Enzymology Series ;
|v v.Volume 710
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|a Description based on publisher supplied metadata and other sources.
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| 505 |
8 |
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|a Front Cover -- Series Page -- Methods in Enzymology -- Copyright -- Contents -- Contributors -- Preface -- Chapter One: Leveraging Saccharomyces cerevisiae for ADAR research: From high-yield purification to high-throughput screening and therapeutic applications -- 1 Introduction -- 2 High-yield purification of ADARs from Saccharomyces cerevisiae for advanced structural and functional studies -- 3 High-throughput screening in yeast promotes ADAR structure-function relationships and substrate preferences -- 4 Yeast-based screening system for optimizing guide RNA sequences that promote the RNA editing of disease-causing mutations -- 5 Exogenous expression of heterologous ADARs in yeast -- References -- Chapter Two: Structural analysis of human ADAR2-RNA complexes by X-ray crystallography -- 1 Introduction -- 2 Synthesis and purification of RNAs -- 2.1 Equipment -- 2.2 Materials -- 2.3 Synthesis and purification procedure -- 3 hADAR2 overexpression and purification for crystallization -- 3.1 Equipment -- 3.2 Materials -- 3.3 Large scale growth of BCY123 cells expressing hADAR2 -- 3.4 First Ni-NTA purification of hADAR2 -- 3.5 His-tag cleavage of hADAR2 via Tobacco Etch Virus (TEV) protease -- 4 TEV overexpression, purification, and storage -- 4.1 Equipment -- 4.2 Materials -- 4.3 Expression of TEV protocol
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| 520 |
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|a This volume focuses on methods and advancements in the study of Adenosine Deaminases Acting on RNA (ADARs), enzymes critical for RNA editing processes. It provides detailed protocols and methodologies for structural and functional analyses, purification techniques, and RNA editing applications in various biological and medical contexts. The book includes insights into ADAR isoforms, their specificity, and their physiological roles through mouse models. It also discusses advanced sequencing technologies, bioinformatics approaches, and tools for detecting and quantifying RNA editing sites. Aimed at researchers and practitioners in molecular biology, chemistry, and related fields, the text serves as a comprehensive resource for understanding and leveraging ADAR mechanisms in therapeutic and experimental settings.
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| 650 |
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|a RNA editing.
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| 650 |
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|a Adenosine deaminase.
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|i Print version:
|a Beal, Peter
|t ADARs
|d Chantilly : Elsevier Science & Technology,c2025
|z 9780443315848
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| 830 |
|
0 |
|a Methods in Enzymology Series.
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| 856 |
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|u http://proxy.library.tamu.edu/login?url=https://www.sciencedirect.com/science/bookseries/00766879/710
|z Connect to the full text of this electronic book
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|a Elsevier ScienceDirect 2026-2027
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|a Texas A&M University
|b College Station
|c Electronic Resources
|s www_evans
|d Available Online
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|e QH450.A33
|h Library of Congress classification
|
| 998 |
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