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Chemical and genomic methods in nucleic acid biology /

Chemical and Genomic Methods in Nucleic Acid Biology, Volume 704 highlights new advances in the field of nucleic acids, with this new volume presenting interesting chapters written by an international board of authors. Specific chapters in this new release include A real-time FRET-based biochemical...

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Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Eichman, Brandt (Editor)
Format: eBook
Language:English
Published: [Place of publication not identified] : Academic Press, 2024.
Series:Methods in Enzymology ; volume 705
Subjects:
Nucleic acids.
Genomics.
Génomique.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Cover image
  • Title page
  • Table of Contents
  • Series Page
  • Copyright
  • Contributors
  • Preface
  • Chapter One: Experimental considerations for precise RNA-mediated insertion of transgenes
  • Abstract
  • 1 Introduction
  • 2 The right transposable element for the task
  • 3 R2p mRNA design
  • 4 Template RNA design
  • 5 PRINT RNA synthesis
  • 6 Co-transfection of PRINT RNAs and cell harvest
  • 7 Analysis of PRINTed cells
  • 8 Future optimizations and applications
  • References
  • Chapter Two: Expression and purification of cell-penetrating Cas9 and Cas12a enzymes for peptide-assisted genome editing
  • Abstract
  • 1 Introduction
  • 2 Expression and purification protocol
  • 3 Summary
  • Acknowledgments
  • References
  • Chapter Three: A user guide to RT-based mapping of RNA modifications
  • Abstract
  • 1 Introduction
  • 2 Using MarathonRT to map modifications
  • 3 Modification validation methods and controls