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Mitochondrial Translocases Part A.

This book provides a comprehensive guide to the isolation, analysis, and processing of mitochondrial proteins. It covers various methods for isolating mitochondria from yeast and mammalian cells, including differential and affinity purification. The book explores subcellular fractionation techniques...

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Bibliographic Details
Main Author: Wiedemann, Nils
Corporate Author: ScienceDirect (Online service)
Format: eBook
Language:English
Published: Chantilly : Elsevier Science & Technology, 2024.
Edition:1st ed.
Series:Methods in Enzymology Series.
Subjects:
Mitochondria.
Proteins.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Title of Book
  • Cover image
  • Title page
  • Table of Contents
  • Series Page
  • Copyright
  • Contributors
  • Preface
  • Section 1 Isolation of mitochondria
  • Chapter One: Isolation of yeast mitochondria by differential centrifugation
  • Abstract
  • 1 Introduction
  • 2 Before you begin
  • 3 Key resources table
  • 4 Materials and equipment
  • 5 Step-by-step method details
  • 6 Expected outcomes for isolated yeast mitochondria
  • 7 Advantages
  • 8 Limitations
  • 9 Optimization and troubleshooting
  • 10 Safety considerations and standards
  • Acknowledgments
  • References
  • Chapter Two: Isolation of yeast mitochondria by affinity purification using magnetic beads
  • Abstract
  • 1 Introduction
  • 2 Before you begin
  • 3 Key resources table
  • 4 Materials and equipment
  • 5 Step-by-step method details