Mitochondrial Translocases Part A.
This book provides a comprehensive guide to the isolation, analysis, and processing of mitochondrial proteins. It covers various methods for isolating mitochondria from yeast and mammalian cells, including differential and affinity purification. The book explores subcellular fractionation techniques...
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| Format: | eBook |
| Language: | English |
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Chantilly :
Elsevier Science & Technology,
2024.
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| Edition: | 1st ed. |
| Series: | Methods in Enzymology Series.
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| Online Access: | Connect to the full text of this electronic book |
| Summary: | This book provides a comprehensive guide to the isolation, analysis, and processing of mitochondrial proteins. It covers various methods for isolating mitochondria from yeast and mammalian cells, including differential and affinity purification. The book explores subcellular fractionation techniques, the prediction and analysis of mitochondrial targeting signals, and methods for monitoring mitochondrial gene expression and protein translocation. Additionally, it discusses the biogenesis of mitochondria and the localization of proteins through genetic screens and imaging techniques. Intended for researchers and students in the field of cellular and molecular biology, it offers a detailed overview of experimental protocols and methodologies for studying mitochondrial function and protein interactions. |
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| Physical Description: | 1 online resource (521 pages). |
| ISBN: | 9780443293313 0443293317 9780443293306 0443293309 |