Laccase and polyphenol oxidase : biochemistry and biotechnological. applications /
This book explores the biochemistry and applications of laccase and polyphenol oxidases, providing a comprehensive overview of their structures, reaction mechanisms, and practical uses in various industries. Edited by Ivanhoe K.H. Leung, the volume covers topics such as the enzymatic activity of the...
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| Format: | eBook |
| Language: | English |
| Published: |
London ; San Diego, CA :
Academic Press, an imprint of Elsevier,
[2025]
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| Series: | Foundations and frontiers in enzymology
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| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Front Cover
- Laccase and Polyphenol Oxidase
- Foundations and frontiers in enzymology series
- Front Matter
- Laccase and Polyphenol OxidaseFoundations and Frontiers in Enzymology Biochemistry and Biotechnological Applications
- Copyright
- Contents
- Contributors
- About the editor
- Preface
- 1
- Functional roles of polyphenol oxidases in plants and fungi
- Introduction
- Polyphenol oxidase structure and mechanism
- Enzymatic activity and reaction mechanisms of polyphenol oxidases
- Latency and activation of polyphenol oxidases
- Polyphenol oxidase and browning
- Strategies to combat enzymatic browning
- Polyphenol oxidase functions against biotic stress in plants
- Polyphenol oxidase function against plant pathogens
- Polyphenol oxidase function against insect pests
- Functions of polyphenol oxidases in plants against abiotic stress
- Biological functions of polyphenol oxidases in plant metabolism synthesis
- Betalain biosynthesis
- Aurone biosynthesis
- Tyrosine and esculetin metabolism
- Lignan biosynthesis
- Functions of polyphenol oxidases in fungi
- Pigmentation and browning
- Lignin degradation
- Morphogenesis
- Fungal defense and interaction
- Polyphenol oxidase genes and characterization
- Conclusions
- References
- 2
- Plant PPO: Production, purification, and activity assays
- Introduction
- Overall PPO structure
- Structural information on the substrate specificity
- Previous investigations on plant PPO purification and characterization
- Crude extraction and purification of PPOs
- Characterization of plant PPOs
- Recombinant plant PPOs: Production, purification and characterization
- Prior investigations into recombinant PPOs
- Case study: Recombinant production, purification and characterization of grape PPO
- Cloning procedures and protein production
- Protein purification.
- Identification of recombinant grape PPO
- Characterization of grape PPO
- Development of a UV/Vis assay for PPO activities
- Conclusions
- References
- 3
- Laccase and polyphenol oxidase: Biochemistry and biotechnological applications
- Introduction
- Structure and catalytic site
- Mechanism of enzyme action
- Biochemical applications of polyphenol oxidase and laccase
- Biosensing/biochemical sensing
- Removal of pollutants
- Other biotechnological applications
- Food industry
- Textile industry
- Pharmaceutical industry
- Bioremediation
- Conclusions and future prospectives
- References
- 4
- Recent advances in the design of PPO inhibitors
- Introduction
- Polyphenol oxidase inhibitors in food packaging systems
- The role of food packaging on enzymatic browning
- Active packaging as an innovative approach to inhibit enzymatic browning
- Choice between plant-based and synthetic PPO inhibitors in active packaging
- Compatibility of plant-based inhibitors with active packaging
- Polyphenol oxidase inhibitors in cosmeceuticals
- Role of tyrosinase in melanogenesis
- Melanogenesis-related dermatological issues
- Plant-based cosmeceutical PPO inhibitors
- Delivery systems for plant-based PPO inhibitors
- Ethical screening methods for melanogenesis inhibition
- Conclusion
- References
- Further reading
- 5
- Reaction mechanism and redox potential of laccase
- Introduction
- laccase as versatile biocatalysts
- Basic structural features of laccases
- Multidomain structure of laccase
- Active site characteristics
- Conserved sequential regions
- Reaction mechanism of laccases and their unique substrate binding cavity
- Substrate oxidation
- Distinction between laccases and other MCOs
- Oxygen reduction mechanism
- Redox potential of laccases
- Role of the axial ligand
- Other impacting factors.
- Relation between redox potential and substrate specificity
- Laccase engineering and the role of redox mediators
- Factors influencing laccase activity other than redox potential
- Conclusion
- References
- 6
- Laccases: Biological functions and potential applications
- Introduction
- Enzyme structure and reaction
- Laccase occurrence and characteristics
- Plant laccases
- Fungal laccases
- Bacterial laccases
- Insect laccases
- Biological functions of laccases
- Synthesis and degradation of lignin
- Pigment formation and polyphenol oxidation
- Pathogen defence
- Biological functions of insect laccase
- Biotechnological and industrial application of laccases
- Biodegradation and bioremediation
- Pulp and paper industry
- Biosensors nanobiotechnology
- Biofuels
- Application in the food industry
- Cosmetic and pharmaceutical manufacturing
- Conclusions
- References
- 7
- Challenges and applications of laccase in bioremediation
- Introduction
- Laccase structures and active sites
- Laccases redox potential and substrate range
- Benefits and drawbacks of current laccase degradation systems
- Free laccase systems
- Laccase-mediator systems
- Immobilized laccase systems
- Fungal versus bacterial laccases for biotechnological applications
- Laccase-assisted bioremediation strategies
- Dye decolorization of textile effluents
- Detoxification of pulp and paper mill effluents
- Pesticides
- Endocrine-disrupting chemicals
- Pharmaceuticals
- Polycyclic aromatic hydrocarbons
- Challenges associated with the laccase-assisted bioremediation of emerging contaminants and future perspectives
- Concluding remarks
- References
- 8
- Immobilization of laccase for industrial wastewater treatment: Current challenges and future perspectives
- Introduction
- Laccase-catalyzed oxidation of substrates
- Laccase mediator system.
- Cross-coupling and polymerization of substrates
- Immobilization of laccase
- Adsorption
- Entrapment/encapsulation
- Cross-linking
- Covalent immobilization
- Multi-enzyme immobilization
- Combination of laccase-catalyzed oxidation and photocatalysis
- Factors influencing laccase immobilization
- Physiochemical properties of laccases
- Immobilization carriers
- Carbon-based materials
- Carbon-based materials
- Minerals
- Minerals
- Silica
- Silica
- Metal oxides
- Metal oxides
- Organic materials
- Organic materials
- Composite materials
- Composite materials
- Immobilization conditions
- Enzyme precipitation
- effects of precipitants
- Enzyme precipitation
- effects of precipitants
- Effects of cross-linkers
- Effects of cross-linkers
- Effects of pH, ionic strength, and temperature
- Effects of pH, ionic strength, and temperature
- Immobilization time
- Immobilization time
- Reactor configurations for laccase-based treatment of wastewater
- Costs and competitiveness of laccase-based treatment of wastewater
- (Eco)toxicity of immobilization carrier materials
- Challenges and future outlook
- Conclusion
- References
- 9
- In silico bioprospecting and engineering laccases: Methods and applications
- Introduction
- Molecular determinants of reactivity in laccases
- Characterization of laccase activity. The computational protocol
- In silico laccase engineering, putting the protocol to work
- On demand bioprospecting with our computational protocol
- Keep an eye on AI
- Property design, not everything is about high catalytic rates
- Conclusion
- References
- Author Index
- Subject Index
- A
- B
- C
- D
- E
- F
- G
- H
- I
- L
- M
- N
- O
- P
- R
- S
- T
- U.
- V
- W
- Back Cover.