http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
biochemistry and biotechnological. applications
This book explores the biochemistry and applications of laccase and polyphenol oxidases, providing a comprehensive overview of their structures, reaction mechanisms, and practical uses in various industries. Edited by Ivanhoe K.H. Leung, the volume covers topics such as the enzymatic activity of these oxidases, their roles in plant and fungal metabolism, and their potential applications in fields like wastewater treatment, food packaging, and the textile industry. The work is aimed at researchers and practitioners in biochemistry and biotechnology, offering insights into the latest advancements in enzyme engineering and inhibitor design. Contributions from various experts highlight innovations in enzymology and the potential for these enzymes to address environmental and industrial challenges.
Front Cover -- Laccase and Polyphenol Oxidase -- Foundations and frontiers in enzymology series -- Front Matter -- Laccase and Polyphenol OxidaseFoundations and Frontiers in Enzymology Biochemistry and Biotechnological Applications -- Copyright -- Contents -- Contributors -- About the editor -- Preface -- 1 -- Functional roles of polyphenol oxidases in plants and fungi -- Introduction -- Polyphenol oxidase structure and mechanism -- Enzymatic activity and reaction mechanisms of polyphenol oxidases -- Latency and activation of polyphenol oxidases -- Polyphenol oxidase and browning -- Strategies to combat enzymatic browning -- Polyphenol oxidase functions against biotic stress in plants -- Polyphenol oxidase function against plant pathogens -- Polyphenol oxidase function against insect pests -- Functions of polyphenol oxidases in plants against abiotic stress -- Biological functions of polyphenol oxidases in plant metabolism synthesis -- Betalain biosynthesis -- Aurone biosynthesis -- Tyrosine and esculetin metabolism -- Lignan biosynthesis -- Functions of polyphenol oxidases in fungi -- Pigmentation and browning -- Lignin degradation -- Morphogenesis -- Fungal defense and interaction -- Polyphenol oxidase genes and characterization -- Conclusions -- References -- 2 -- Plant PPO: Production, purification, and activity assays -- Introduction -- Overall PPO structure -- Structural information on the substrate specificity -- Previous investigations on plant PPO purification and characterization -- Crude extraction and purification of PPOs -- Characterization of plant PPOs -- Recombinant plant PPOs: Production, purification and characterization -- Prior investigations into recombinant PPOs -- Case study: Recombinant production, purification and characterization of grape PPO -- Cloning procedures and protein production -- Protein purification.
Identification of recombinant grape PPO -- Characterization of grape PPO -- Development of a UV/Vis assay for PPO activities -- Conclusions -- References -- 3 -- Laccase and polyphenol oxidase: Biochemistry and biotechnological applications -- Introduction -- Structure and catalytic site -- Mechanism of enzyme action -- Biochemical applications of polyphenol oxidase and laccase -- Biosensing/biochemical sensing -- Removal of pollutants -- Other biotechnological applications -- Food industry -- Textile industry -- Pharmaceutical industry -- Bioremediation -- Conclusions and future prospectives -- References -- 4 -- Recent advances in the design of PPO inhibitors -- Introduction -- Polyphenol oxidase inhibitors in food packaging systems -- The role of food packaging on enzymatic browning -- Active packaging as an innovative approach to inhibit enzymatic browning -- Choice between plant-based and synthetic PPO inhibitors in active packaging -- Compatibility of plant-based inhibitors with active packaging -- Polyphenol oxidase inhibitors in cosmeceuticals -- Role of tyrosinase in melanogenesis -- Melanogenesis-related dermatological issues -- Plant-based cosmeceutical PPO inhibitors -- Delivery systems for plant-based PPO inhibitors -- Ethical screening methods for melanogenesis inhibition -- Conclusion -- References -- Further reading -- 5 -- Reaction mechanism and redox potential of laccase -- Introduction -- laccase as versatile biocatalysts -- Basic structural features of laccases -- Multidomain structure of laccase -- Active site characteristics -- Conserved sequential regions -- Reaction mechanism of laccases and their unique substrate binding cavity -- Substrate oxidation -- Distinction between laccases and other MCOs -- Oxygen reduction mechanism -- Redox potential of laccases -- Role of the axial ligand -- Other impacting factors.
Relation between redox potential and substrate specificity -- Laccase engineering and the role of redox mediators -- Factors influencing laccase activity other than redox potential -- Conclusion -- References -- 6 -- Laccases: Biological functions and potential applications -- Introduction -- Enzyme structure and reaction -- Laccase occurrence and characteristics -- Plant laccases -- Fungal laccases -- Bacterial laccases -- Insect laccases -- Biological functions of laccases -- Synthesis and degradation of lignin -- Pigment formation and polyphenol oxidation -- Pathogen defence -- Biological functions of insect laccase -- Biotechnological and industrial application of laccases -- Biodegradation and bioremediation -- Pulp and paper industry -- Biosensors nanobiotechnology -- Biofuels -- Application in the food industry -- Cosmetic and pharmaceutical manufacturing -- Conclusions -- References -- 7 -- Challenges and applications of laccase in bioremediation -- Introduction -- Laccase structures and active sites -- Laccases redox potential and substrate range -- Benefits and drawbacks of current laccase degradation systems -- Free laccase systems -- Laccase-mediator systems -- Immobilized laccase systems -- Fungal versus bacterial laccases for biotechnological applications -- Laccase-assisted bioremediation strategies -- Dye decolorization of textile effluents -- Detoxification of pulp and paper mill effluents -- Pesticides -- Endocrine-disrupting chemicals -- Pharmaceuticals -- Polycyclic aromatic hydrocarbons -- Challenges associated with the laccase-assisted bioremediation of emerging contaminants and future perspectives -- Concluding remarks -- References -- 8 -- Immobilization of laccase for industrial wastewater treatment: Current challenges and future perspectives -- Introduction -- Laccase-catalyzed oxidation of substrates -- Laccase mediator system.
Cross-coupling and polymerization of substrates -- Immobilization of laccase -- Adsorption -- Entrapment/encapsulation -- Cross-linking -- Covalent immobilization -- Multi-enzyme immobilization -- Combination of laccase-catalyzed oxidation and photocatalysis -- Factors influencing laccase immobilization -- Physiochemical properties of laccases -- Immobilization carriers -- Carbon-based materials -- Carbon-based materials -- Minerals -- Minerals -- Silica -- Silica -- Metal oxides -- Metal oxides -- Organic materials -- Organic materials -- Composite materials -- Composite materials -- Immobilization conditions -- Enzyme precipitation -- effects of precipitants -- Enzyme precipitation -- effects of precipitants -- Effects of cross-linkers -- Effects of cross-linkers -- Effects of pH, ionic strength, and temperature -- Effects of pH, ionic strength, and temperature -- Immobilization time -- Immobilization time -- Reactor configurations for laccase-based treatment of wastewater -- Costs and competitiveness of laccase-based treatment of wastewater -- (Eco)toxicity of immobilization carrier materials -- Challenges and future outlook -- Conclusion -- References -- 9 -- In silico bioprospecting and engineering laccases: Methods and applications -- Introduction -- Molecular determinants of reactivity in laccases -- Characterization of laccase activity. The computational protocol -- In silico laccase engineering, putting the protocol to work -- On demand bioprospecting with our computational protocol -- Keep an eye on AI -- Property design, not everything is about high catalytic rates -- Conclusion -- References -- Author Index -- Subject Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- L -- M -- N -- O -- P -- R -- S -- T -- U.
Includes bibliographical references and index.