Concepts and techniques in omics and system biology /
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| Format: | eBook |
| Language: | English |
| Published: |
London ; San Diego, CA :
Academic Press,
[2024]
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| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Front Cover
- Concepts and Techniques in OMICS and System Biology
- Concepts and Techniques in OMICS and System Biology
- Copyright
- Dedication
- Contents
- About the authors
- Preface
- Acknowledgments
- 1
- Introduction to proteomics, metabolomics, and ionomics
- Introduction
- Proteomics techniques-Low throughput and high throughput techniques
- Low-throughput techniques
- High-throughput techniques
- Introduction to metabolomics
- Introduction to ionomics
- Applications of proteomics
- Agriculture
- Food science
- Medicine
- Systems biology
- Paleo-proteomics
- Applications of metabolomics
- Metabolomics in plant development
- Metabolomics during plant stress
- Application of metabolomics for genetically modified (GM) crops
- Metabolomics and crop improvement
- Applications of ionomics
- Ionomics in normal plant growth
- Ionomics and plant biotic stress
- Ionomics and abiotic stress in crops
- Quantitative trait loci (QTLs) regulating ionomics
- Conclusion
- References
- 2
- Proteomics and the techniques applicable
- Introduction
- Protein primary structure
- Protein secondary structure
- Hydrogen bond
- Psi and phi angles
- Unusual secondary structures
- Motifs
- Tertiary protein structure
- Protein domains
- Quaternary protein structure
- Protein structure determination by X-ray crystallography
- Requirements
- X-ray generation
- X-ray diffraction
- X-ray detector
- NMR
- Protein structure determination using NMR
- Principles and applications of mass spectrometry
- Principles of Mass spectrometry
- Applications of Mass spectrometry (Pukala & Robinson, 2022)
- Proteomics: Basic understanding
- Gel-Based proteomics
- Gel-free proteomics
- iTRAQ
- Conclusion
- References
- Further reading
- 3
- Advances in proteomics techniques
- Introduction
- MASCOT
- pQuant software
- Trans Proteomic Pipeline (TPP) software
- Census software
- MaxQuant software
- Advanced features
- Exploring proteome databases
- UniProt
- NCBI protein
- Ensembl
- Protein Data Bank
- PeptideAtlas
- Human protein Atlas
- PRIDE database
- Human proteome organization proteomics standards initiative repositories
- RefSeq
- SWISS-MODEL repository
- Conclusion
- References
- Further reading
- 4
- Aspects of protein interactions
- Coimmuno precipitation
- Antibody selection
- Cell lysis
- Preclearing
- Immunoprecipitation
- Washing
- Elution
- Analysis
- Applications
- Pull-down assays
- Analysis
- Cross-linking, label transfer and far-Western blot analysis
- Conclusion
- References
- 5
- Metabolomics and the techniques applicable
- Introduction
- Objective
- Methods of analysis
- Biological applications
- Challenges
- Use of 1D NMR in metabolomic analysis
- Quantitative analysis
- Structural elucidation
- Dynamic studies
- Metabolic profiling
- Biomarker discovery
- Stable isotope tracing
- Quality control in bioprocessing
- Environmental metabolomics