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Evolving landscape of molecular diagnostics : applications and techniques /

This comprehensive book explores the evolving landscape of molecular diagnostics, covering a wide range of applications and techniques. Edited by Mrutyunjay Suar, Namrata Misra, and Prashant Kumar Singh, it provides insights into the past, present, and future of molecular diagnostics, discussing key...

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Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Suar, Mrutyunjay (Editor), Singh, Prashanth (Editor), Misra, Namrata (Editor)
Format: eBook
Language:English
Published: Amsterdam, Netherlands : Elsevier, 2024.
Subjects:
Molecular diagnosis.
Diagnostics moléculaires.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Front Cover
  • Evolving Landscape of Molecular Diagnostics
  • Copyright Page
  • Dedication
  • Contents
  • List of contributors
  • About the editors
  • Acknowledgment
  • Introduction
  • 1 Scope and scientific emphasis of each chapter
  • 2 Utility and distinctiveness of the book
  • A. Fundamentals of molecular diagnostics: from past to present to future
  • 1 Molecular diagnostics: past, present, and future
  • 1.1 Introduction
  • 1.2 The past, the beginning, and the early milestones
  • 1.2.1 Polymerase chain reaction amplification-based assayes
  • 1.2.2 Sequencing-based assays
  • 1.2.3 The present state of other significant approaches
  • 1.2.3.1 Surface-enhanced Raman scattering
  • 1.2.3.2 Nanopore system-based diagnosis
  • 1.2.3.3 Isothermal amplifications
  • 1.2.3.4 Exploiting CRISPR
  • 1.2.3.5 Signal-mediated amplification of RNA technology
  • 1.2.4 Point-of-care devices
  • 1.3 Conclusion
  • References
  • 2 Molecular diagnostics for bacteria, virus, and fungi
  • 2.1 Introduction
  • 2.2 Molecular methods for microbial diagnosis
  • 2.3 Clinical virology and molecular diagnostics
  • 2.3.1 Real-time quantitative polymerase chain reaction
  • 2.3.2 Enzyme-linked immunosorbent assay
  • 2.3.3 Next-generation sequencing
  • 2.3.4 SHERLOCK assay technique
  • 2.3.5 Heating unextracted diagnostic samples to obliterate nuclease
  • 2.3.6 Rapid diagnostic test
  • 2.3.7 Antibody-dependent detection
  • 2.3.8 Neutralization assay
  • 2.3.9 Chemiluminescent immunoassay
  • 2.4 Conclusion
  • References
  • B. Emerging diagnostics techniques: which one to choose?
  • 3 MALDI TOF-MS for microbial identification and diagnosis
  • 3.1 Introduction
  • 3.2 Principle and methodology of MALDI TOF-MS
  • 3.3 Advantages of MALDI-TOF-MS in comparison to alternative methods for microbial identification
  • 3.4 Application of MALDI TOF-MS
  • 3.4.1 Microbial identification.
  • 3.4.2 Microbial taxonomy
  • 3.4.3 Strain typing and subtyping
  • 3.4.4 Antimicrobial resistance profiling
  • 3.4.5 Quality control in industrial and research settings
  • 3.4.6 Forensic microbiology: criminal investigations
  • 3.5 Clinical application for the identification, taxonomy, and strain typing of bacteria and fungi
  • 3.5.1 MS identification of bacteria
  • 3.5.2 MS identification of fungi
  • 3.6 Other examples of MALDI-TOF-MS applications in the clinical diagnosis
  • 3.6.1 Bioterrorism
  • 3.6.2 Exploring antimicrobial resistance in bacterial pathogens through MALDI-TOF analysis
  • 3.6.3 Use of MALDI-TOF for epidemiology-related studies
  • 3.7 Conclusion
  • References
  • 4 Microfluidics in diagnostic research: lab-on-a-chip technologies
  • 4.1 Introduction
  • 4.2 Manipulating the fluid at microscale: microfluidic design
  • 4.2.1 Diffusion
  • 4.2.2 Laminar flow
  • 4.2.3 Surface tension
  • 4.2.4 Rapid heating and cooling
  • 4.2.5 Electrokinetics
  • 4.2.6 Interaction between two different phases
  • 4.3 Fabrication of microfluidic devices
  • 4.3.1 Paper
  • 4.3.2 Polymer
  • 4.3.3 Threads
  • 4.3.4 Hydrogels
  • 4.4 Applications of microfluidic devices
  • 4.4.1 Organ-on-a-Chip
  • 4.4.2 Cell-based assays
  • 4.4.3 Bioprinting
  • 4.4.4 Microvalves
  • 4.4.5 Micropumps
  • 4.4.6 Micromixers
  • 4.4.7 Drug screening
  • 4.4.8 Energy devices
  • 4.5 Lab-on-a-Chip devices in laboratory
  • 4.5.1 Pathogen detection using microfluidics
  • 4.5.2 Point-of-care devices
  • 4.5.3 Microfluidic-based eHealth
  • 4.6 Commercially available microfluidic systems
  • 4.7 Way forward
  • References
  • Further reading
  • 5 TaqMan low-density arrays for simultaneous detection of multiple pathogens
  • 5.1 Introduction
  • 5.2 TaqMan low-density array
  • 5.3 Design of TaqMan low-density array card
  • 5.4 TaqMan low-density array workflow.
  • 7.2 Types and characteristics of quantum dots
  • 7.3 Optical properties of quantum dots
  • 7.4 Synthesis and characterization of quantum dots
  • 7.4.1 Synthesis of quantum dots
  • 7.4.2 Characterization of quantum dots
  • 7.5 Functionalization and solubilization
  • 7.5.1 Silanization
  • 7.5.2 Ligand exchange
  • 7.5.3 Amphiphilic ligand encapsulation
  • 7.5.4 Bioconjugation
  • 7.6 In vitro diagnostic applications
  • 7.6.1 Immunolabeling
  • 7.6.2 Nucleic acid detection
  • 7.6.3 Cellular imaging
  • 7.6.3.1 In vitro molecular interaction studies
  • 7.6.3.1.1 Superresolution microscopy
  • 7.6.3.1.2 Single-particle tracking
  • 7.6.3.1.3 In vitro cellular imaging
  • 7.6.3.1.4 In vivo imaging
  • 7.6.4 Quantum dot-based tumor targeting and Imaging
  • 7.6.5 Bimodal luminescent-magnetic quantum dots for imaging
  • 7.7 Other biological applications of quantum dots
  • 7.7.1 Immunohistofluorescence
  • 7.7.2 Fluorescence in situ hybridization
  • 7.7.3 Homogeneous energy transfer assays
  • 7.7.4 Pathogen and toxin detection
  • 7.8 Conclusion
  • References
  • 8 Loop-mediated isothermal amplification as a point of care diagnostic tool
  • 8.1 Revolutionizing diagnostics: the loop-mediated isothermal amplification technology
  • 8.2 The mechanism of loop-mediated isothermal amplification in diagnostics
  • 8.3 Empowering diagnostics: features of loop-mediated isothermal amplification technology
  • 8.4 The versatility of loop-mediated isothermal amplification and its dynamic variants
  • 8.4.1 Individual gene identification
  • 8.4.2 Multiplex-loop-mediated isothermal amplification: parallel gene identification
  • 8.4.3 Reverse transcriptase loop-mediated isothermal amplification
  • 8.4.4 Digital loop-mediated isothermal amplification
  • 8.4.5 Paper-based loop-mediated isothermal amplification
  • 8.4.6 Micro-loop-mediated isothermal amplification.
  • 8.5 Enhancing precision: approaches to monitor loop-mediated isothermal amplification results
  • 8.5.1 Gel electrophoresis
  • 8.5.2 Fluorescence
  • 8.5.3 Turbidity
  • 8.5.4 Naked-eye monitoring
  • 8.5.4.1 Naked-eye monitoring with DNA-binding dyes
  • 8.5.4.2 Naked-eye monitoring using colorimetric indicators
  • 8.6 Loop-mediated isothermal amplification: illuminating disease diagnosis with speed and precision
  • 8.7 Loop-mediated isothermal amplification: revolutionizing diagnostics with cutting-edge advances
  • 8.7.1 Breaking barriers: the latest innovations in reverse transcription loop-mediated isothermal amplification
  • 8.7.2 Recent strides in multiplex-loop-mediated isothermal amplification
  • 8.7.3 Unlocking current trends in digital loop-mediated isothermal amplification
  • 8.7.4 CRISPR-loop-mediated isothermal amplification
  • 8.8 Shaping the future of diagnostics: advantages of loop-mediated isothermal amplification technology
  • 8.9 Overcoming hurdles: challenges in harnessing the full potential of loop-mediated isothermal amplification
  • 8.10 Loop-mediated isothermal amplification technology has the potential to lead the way in diagnostics
  • References
  • 9 Novel diagnostics techniques for detection of Coronavirus disease 2019
  • 9.1 Background
  • 9.2 Targets for coronavirus disease 2019 detection (viral antigen, antibodies, and RNA)
  • 9.3 Molecular-based testing methods
  • 9.3.1 Rapid direct detection of SARS-CoV-2 using immunochromatography method
  • 9.3.2 Detection of SARS-CoV-2 using CRISPR system
  • 9.3.2.1 Sample collection
  • 9.3.2.2 RNA extraction
  • 9.3.2.3 Amplification and detection
  • 9.3.3 Detection of SARS-CoV-2 using reverse transcription-loop-mediated isothermal amplification
  • 9.3.4 Detection of SARS-CoV-2 using quantitative reverse transcription-polymerase chain reaction
  • 9.3.4.1 Viral RNA extraction.