Cover Image

Peptide and Peptide Mimicking Tools.

This book, part of the 'Methods in Enzymology' series, focuses on the synthesis, design, and application of peptides and peptide-mimicking tools in enzymology. It covers various methods of peptide synthesis, including late-stage modifications, chemical cyclization, and selective chemical m...

Full description

Bibliographic Details
Main Author: Chenoweth, David M.
Corporate Author: ScienceDirect (Online service)
Other Authors: David Chenoweth
Format: eBook
Language:Aramaic
Published: San Diego : Elsevier Science & Technology, 2024.
Edition:1st ed.
Series:Issn Series.
Subjects:
Peptides > Synthesis.
Peptide drugs.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Front Cover
  • Series Page
  • Methods in Enzymology
  • Copyright
  • Contents
  • Contributors
  • Chapter One: Synthesis, derivatization, and conformational scanning of peptides containing N-Aminoglycine
  • 1 Introduction
  • 1.1 Conformational effects of Gly N-Amination in peptides
  • 1.2 Late-stage modification of N-Aminopeptides
  • 2 Protocols for the synthesis of aGly-containing peptides
  • 2.1 Reagents and equipment
  • 2.2 Synthesis of Fmoc-protected amino acid chlorides
  • 2.3 General procedure for synthesis of aGly-containing peptides on solid support
  • Chapter Two: Thioimidates provide general access to thioamide, amidine, and imidazolone peptide-bond isosteresThioimidates: Access to Thioamide, Amidine, and Imidazolone Peptide-Bond Isosteres
  • 1 Introduction
  • 2 Thioamide peptide-bond isosteres
  • 2.1 Thioamide during Fmoc-deprotection
  • 2.2 Thioamide during peptide cleavage
  • 3 Amidine peptide-bond isosteres
  • 4 (4H)-Imidazolone peptide-bond isosteres
  • 5 Materials
  • 6 Protocol for synthesis of protected thionopeptides
  • 6.1 General solid-phase peptide synthesis protocol
  • 6.2 Synthesis #x200B
  • #x200B
  • Resin installation of thionopeptides via thiobenzotriazolides
  • 6.3 Protection of thionopeptides via resin alkylation
  • 6.4 Synthesis of p-Azidobenzyl bromide
  • 7 Protocol for cleavage #x200B
  • #x200B
  • deprotection of protected thionopeptides
  • 7.1 Resin cleavage of protected thioimidate peptides
  • 7.2 Solution-phase deprotection of p-azidobenzyl thioimidates
  • 8 Protocol for amidinopeptide synthesis via thioimidates
  • 8.1 Resin conversion of thioimidates into amidines
  • 8.2 Resin cleavage of amidino peptides
  • 9 Protocol for backbone imidazolone peptide synthesis via thioimidates
  • 9.1 Synthesis of thioimidate precursor
  • 9.2 Resin installation of backbone imidazolones via thioimidate precursor
  • 9.3 Resin cleavage of backbone imidazolone peptides
  • 10 Summary and conclusions
  • Acknowledgements
  • References
  • Chapter Three: A single backbone amide modification method to achieve single site-specific chemical protein conjugation
  • 1 Introduction
  • 2 Developing CAST for a single peptide backbone amide modification
  • 2.1 Equipment
  • 2.2 Buffers and reagents
  • 2.3 Procedure
  • 2.3.1 Peptide synthesis
  • 2.3.2 Experimental procedures and reaction optimizations