Cover Image

Peptide Catalysts, Including Catalytic Amyloids /

Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Ivan V. Korendovych (Editor)
Format: eBook
Language:English
Published: Cambridge, MA : Academic Press, 2024.
Series:Methods in enzymology ; v. 697.
Subjects:
Peptides.
Catalysts.
Catalyseurs.
catalyst.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Front Cover
  • Series Page
  • Methods in Enzymology
  • Copyright
  • Contents
  • Contributors
  • Preface
  • Chapter One: Avoiding common pitfalls in designing kinetic protocols for catalytic amyloid studies
  • 1 Introduction
  • 2 No turnover-no catalysis!
  • 3 Substrate solubility and its effects on the reported kinetic values
  • 4 Buffer contributions and related potential pitfalls
  • 5 Solution mixing and uniformity in assay design
  • 6 Reliable data through spectrophotometric principles
  • 7 The time zero problem
  • 8 Michaelis-Menten kinetics and derived parameters
  • Acknowledgments
  • Chapter Three: Stopped-flow measurement of CO2 hydration activity by catalytic amyloids
  • 1 Introduction
  • 1.1 Stopped-flow methods of kinetic monitoring
  • 2 General methods
  • 3 Materials
  • 4 Methods
  • 4.1 Peptide synthesis
  • 4.2 Carbon dioxide sample preparation and handling
  • 4.3 CO2 hydration assay
  • 4.3.1 Data analysis and calculation of buffer factor
  • 4.3.1.1 Fitting data to Michaelis-Menten models and extraction of kinetic information
  • 5 Notes
  • Acknowledgments
  • References
  • Chapter Four: Characterization of self-templating catalytic amyloids
  • 1 Introduction
  • 2 General material and equipment requirements
  • 3 Purification, quantitation, storage, and analysis of amyloidogenic peptides
  • 3.1 Peptide purification using reverse-phase HPLC
  • 3.2 Quantitation and storage of purified peptides
  • 3.3 Biophysical and morphological characterization of aggregates
  • 3.4 Analytical quantitation of amyloids in complex mixtures
  • 4 Amyloid templating assays
  • 4.1 Materials and equipment
  • 4.2 Cross-templating amino acid condensation reactions
  • 4.3 Autocatalytic templating reaction with the V(OV)4/(OV)4 amyloids
  • 4.4 Measuring the kinetics of autocatalytic templating amyloids
  • 5 Summary
  • Acknowledgements
  • References
  • Chapter Five: Catalytic physiological amyloids
  • 1 Introduction
  • 2 Amyloid fibril preparation and characterization
  • 2.1 Amyloid sample preparation
  • 3 Key resources table
  • 4 Materials and equipment
  • 4.1 Materials
  • 4.2 Equipment
  • 5 Step-by-step method details
  • 6 Expected outcomes
  • 7 Limitations
  • 8 Optimization and troubleshooting
  • 8.1 Characterization of amyloids
  • 8.1.1 Amyloid-specific markers
  • 8.1.2 Secondary structure analysis