Environmental metagenomics, water quality and possible remediation measures of polluted waters : a combined approach /
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| Format: | eBook |
| Language: | English |
| Published: |
Amsterdam ; Cambridge, MA :
Elsevier,
[2024]
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| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Front cover
- Half title
- Title page
- Copyright
- Dedication
- Contents
- Contributors
- About the editors
- Preface
- Acknowledgments
- Chapter 1 Aqueous ecosystem: Environmental metagenomics, water quality, and possible remediation measures
- 1.1 Introduction
- 1.2 Environmental metagenomics protocols
- 1.2.1 Study design and sample collection
- 1.2.2 DNA extraction
- 1.2.3 DNA sequencing and bioinformatics
- 1.3 Linking metagenomics to water quality, biogeochemical cycles, and bioremediation
- 1.4 Conclusion and future recommendations
- Acknowledgment
- References
- Chapter 2 Environmental metagenomics and associated functional annotations in the polluted ecosystem: Antimicrobial resistance perspective
- 2.1 Introduction
- 2.2 Importance of environmental antimicrobial resistance
- 2.3 Tools for metagenomic analysis and relevance
- 2.3.1 Sampling, Metadata and DNA extraction
- 2.3.2 Sequencing, quality control, and trimming
- 2.3.3 Library preparation
- 2.3.4 Analysis of NGS sequences
- 2.3.5 Assembly and binning
- 2.3.6 Taxonomy mapping, annotation, and statistical analysis
- 2.4 Metagenomic data
- 2.4.1 Wastewater
- 2.4.2 Surface water
- 2.4.3 Brackish water
- 2.4.4 Other sources of AMR
- 2.5 Application of metagenomic data for AMR mitigation strategy and recent advancements
- 2.6 Future prospects and conclusion
- References
- Chapter 3 Next-generation sequencing technologies for microbial communities' identification and characterization from aquatic and soil ecosystems for integrated management
- 3.1 Introduction
- 3.2 Microbial network of aquatic and soil ecosystem for isolation, identification, and characterization
- 3.3 Genomic and metagenomic DNA isolation protocols of microbiomes from aquatic and soil ecosystem
- 3.4 Sequence platforms/technologies for microbial identification and characterization
- 3.5 NGS library preparation based on emulsion and polony PCR
- 3.5.1 Making NGS library
- 3.6 NGS file format
- 3.7 Microbial identification and characterization using sequence databases
- 3.8 Conclusion
- References
- Chapter 4 Recent advances in the field of trace-level analysis of environmental samples
- 4.1 Introduction
- 4.1.1 Water pollution is a complex problem
- 4.1.2 The aftermath of water pollution
- 4.2 Aquatic monitoring
- 4.2.1 History of aquatic monitoring
- 4.2.2 Major challenges in the monitoring of aquatic samples
- 4.2.3 Classification of water monitoring techniques
- 4.3 Sampling and sample preparation
- 4.3.1 Sampling design and strategies
- 4.3.2 Sampling techniques for soil and water analysis
- 4.3.3 Sample preparation
- 4.3.4 Analysis of metal and other inorganics in water
- 4.3.5 Analysis of organic contaminants in water
- 4.4 Advanced techniques of environmental analysis
- 4.5 Conclusion
- References
- Chapter 5 Detailed overview of techniques used across the globe for the remediation of pollutants from polluted water