Enzymes in RNA science and biotechnology. Part B /
Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRN...
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Amsterdam, Netherlands :
Elsevier Academic Press,
2023.
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| Edition: | First edition. |
| Series: | Methods in enzymology ;
v. 692. |
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
| Summary: | Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins. Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus. |
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| Physical Description: | 1 online resource (xxi, 324 pages) : illustrations (some colour). |
| ISBN: | 9780443239779 0443239770 0443239762 9780443239762 |
| ISSN: | 0076-6879 ; |