Cover Image

CRISPR-CAS system in translational biotechnology /

Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Joshi, Swati, Verma, Digvijay, Gupta, Ravi Kr
Format: eBook
Language:English
Published: [S.l.] : Academic Press, 2023.
Subjects:
CRISPR-associated protein 9 > Biotechnology.
Biotechnology.
Protéine-9 associée à CRISPR > Biotechnologie.
Biotechnologie.
bioengineering.
Biotechnology
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Front Cover
  • CRISPR-Cas System in Translational Biotechnology
  • Copyright Page
  • Contents
  • List of contributors
  • 1 CRISPR-Cas fundamentals and advances
  • 1 CRISPR-Cas9: chronology and evolution
  • 1.1 Introduction
  • 1.2 Discovery of CRISPR-Cas system
  • 1.3 Mechanism of CRISPR-Cas system
  • 1.4 Classification of CRISPR-Cas system
  • 1.4.1 Class I CRISPR-Cas system
  • 1.4.1.1 Type I
  • 1.4.1.2 Type III
  • 1.4.1.3 Type IV
  • 1.4.2 Class II CRISPR-Cas system
  • 1.4.2.1 Type II system
  • 1.4.2.2 Type V system
  • 1.4.2.3 Type VI system
  • 1.5 Limitations of CRISPR technology
  • 1.5.1 CRISPR-Cas9 off-target effects
  • 1.5.2 DNA damage and apoptosis
  • 1.5.3 Immunotoxicity
  • 1.5.4 Delivery of CRISPR system to hosts
  • 1.6 Applications of CRISPR technology
  • 1.6.1 Role of CRISPR in gene regulation
  • 1.6.2 In metabolic engineering
  • 1.6.3 Role of CRISPR in COVID-19
  • 1.6.4 CRISPR for animal model preparation
  • 1.7 Conclusions and future perspectives
  • References
  • 2 CRISPR: the Janus god of modern science
  • 2.1 Introduction
  • 2.2 Timeline of developments in CRISPR-Cas system
  • 2.3 Applications of CRISPR-Cas gene-editing system in human diseases
  • 2.3.1 In the treatment of genetic disorders
  • 2.3.2 In the treatment of various types of cancer
  • 2.3.3 In the treatment of acquired immunodeficiency syndrome
  • 2.3.4 In the treatment of neurodegenerative disorders
  • 2.3.5 In Coronavirus disease 2019
  • 2.4 Applications of CRISPR system in agriculture for crop improvement
  • 2.4.1 Increasing productivity
  • 2.4.2 Quality improvement
  • 2.4.3 Tolerance to biotic and abiotic stress
  • 2.4.4 Herbicide resistance
  • 2.4.5 Expediting hybrid breeding
  • 2.5 Applications of CRISPR-Cas9 system in environmental science
  • 2.5.1 Bioplastic
  • 2.5.2 Biofuel
  • 2.5.3 Biosensors
  • 2.5.4 Bioremediation
  • 2.6 Limitations of CRISPR-Cas9 gene-editing system in medicinal, agricultural, and environmental sectors
  • 2.6.1 Limitations of CRISPR in the medicinal field
  • 2.6.1.1 Mutations
  • 2.6.1.2 Immunotoxicity
  • 2.6.2 Limitations of CRISPR in agricultural crops
  • 2.6.3 Limitations of CRISPR on the environment
  • 2.6.4 Implications for Cas and mitigating strategies
  • 2.7 Conclusions
  • References
  • 3 Commercialization of CRISPR-Cas technology: issues and impact
  • 3.1 Introduction
  • 3.2 CRISPR-Cas IPR dispute: who stands where?
  • 3.3 Worldwide patent landscape of CRISPR
  • 3.4 A commercial conundrum
  • 3.5 Ethical concerns
  • 3.6 Challenges in commercialization of CRISPR-Cas
  • 3.6.1 Agriculture
  • 3.6.2 Medicine
  • 3.7 Conclusions
  • References
  • 2 CRISPR-Cas in medical biotechnology
  • 4 CRISPR-Cas-led advancements in translational biotechnology
  • 4.1 Introduction
  • 4.2 Genome editing tools before CRISPR
  • 4.2.1 Zinc finger nucleases
  • 4.2.2 Transcription activator-like effector nucleases
  • 4.2.3 Adeno-associated viruses
  • 4.3 CRISPR technology
  • 4.3.1 Components of CRISPR
  • 4.3.2 Cas enzymes
  • 4.3.2.1 Cas9