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Genetic engineering and genome editing for zinc biofortification of rice /

Genetic Engineering and Genome Editing for Zinc Biofortification of Rice provides the first single-volume, comprehensive resource on genetic engineering approaches, including novel genome editing techniques, that are carried out in rice, a staple crop for much of the world's population.

Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Swamy, B. P. Mallikarjun (Editor), Macovei, Anca (Editor), Trijatmiko, Kurniawan Rudi (Editor)
Format: eBook
Language:English
Published: Amsterdam : Academic Press, 2023.
Subjects:
Rice > Genetic engineering.
Crop improvement.
Riz > Génie génétique.
Cultures > Amélioration.
Crop improvement
Rice > Genetic engineering
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Front Cover
  • Genetic Engineering and Genome Editing for Zinc Biofortification of Rice
  • Genetic Engineering and Genome Editing for Zinc Biofortification of Rice
  • Copyright
  • Contents
  • List of contributors
  • Foreword
  • Preface
  • 1
  • Molecular mechanisms leading to grain Zn accumulation in rice
  • 1. Introduction
  • 1.1 Genes and gene families associated with Zn metabolism
  • 1.1.1 ZIP (zinc iron-regulated proteins)
  • 1.1.2 Iron regulated transporters (IRTs)
  • 1.1.3 Heavy metal ATPases (HMAs)
  • 1.1.4 Vacuolar iron transporters (VIT)
  • 1.1.5 Natural resistance-associated macrophage protein (NRAMP)
  • 1.1.6 Metal tolerance proteins (MTPs)
  • 1.1.7 Yellow stripe-like (YSL) transporters
  • 1.1.8 Nicotianamine (NA) and mugineic acid (MA) derivatives
  • 1.2 Processes associated with Zn metabolism
  • 1.2.1 Synthesis and/or secretion of chelators for zn
  • 1.2.2 Zn uptake
  • 1.2.3 Genes interaction
  • 1.3 Zn distribution among the rice plant parts including grain/seeds
  • 1.3.1 Root to shoot
  • 1.3.2 Shoot
  • 1.3.3 Nodes
  • 1.3.4 Flag leaf
  • 1.3.5 Grains
  • 1.3.6 Zn storage in plant cells (in vacuoles)
  • 1.3.7 Uptake of cadmium along with zn
  • 2. Conclusions
  • References
  • 2
  • Molecular links between iron and zinc biofortification in rice
  • 1. Introduction
  • 2. Strategies of Fe and Zn uptake in rice
  • 3. Long-distance Fe and Zn transport in rice
  • 3.1 Fe and Zn transport through xylem
  • 3.2 Fe and Zn transport through phloem
  • 3.3 Fe and Zn translocation to seed
  • 4. Fe and Zn biofortification in rice
  • 4.1 Approaches focusing on enhancing the Fe and Zn uptake mechanisms
  • 4.2 Approaches focusing on enhancing the Fe and Zn translocation mechanisms
  • 4.3 Approaches focusing on endosperm-specific Fe and Zn storage
  • 4.4 Increased synthesis of mugineic acids and endosperm-specific Fer expression.
  • 4.5 Other approaches for enhancing Fe and Zn biofortification
  • 5. Conclusions
  • References
  • 3
  • Ionomics-based imaging, localization and quantification of zinc and other micronutrients in rice grains for bio ...
  • 1. Introduction
  • 2. Role of ionomics in Zn biofortification in rice
  • 3. Ionomics for high-throughput elemental analysis integrated with advanced technologies
  • 4. Overview of elemental profiling in rice and other cereal crops
  • 5. Destructive methods of element profiling
  • 6. Nondestructive methods of element profiling
  • 7. Ionomics in Zn biofortification-selected case studies
  • 8. Future prospects for ionomics research in rice biofortification studies
  • 9. Summary and conclusion
  • Acknowledgment
  • References
  • Further reading
  • 4
  • Recent advances in precise plant genome editing technology
  • 1. Introduction
  • 2. CRISPR/Cas system
  • 3. CRISPR reagent delivery methods in plants
  • 4. Dominance of NHEJ-mediated genome editing in somatic cells
  • 5. Precise genome editing tools
  • 5.1 Homology-directed repair (HDR)-mediated genome editing
  • 5.2 Base editors
  • 5.3 Prime editing
  • 6. Conclusion and future perspective
  • 7. Conflict of interest
  • References
  • Further reading
  • 5
  • Practical protocol for design and construction of a transformation vector for prime editing in rice
  • 1. Introduction
  • 2. Design of transformation vector for prime editing in rice
  • 2.1 Materials
  • 2.2 Protocols
  • 3. Construction of transformation vector for prime editing in rice
  • 3.1 Materials
  • 4. Protocols
  • 4.1 Synthesis of level 1 fragment
  • 4.2 Level-1 cloning step
  • 4.3 Level-2 cloning step
  • 4.4 Agrobacterium transformation
  • References
  • 6
  • Stages of development of genetically modified (GM) plants
  • 1. Introduction
  • 2. Gene identification and crop transformation
  • 3. Efficacy trials
  • 4. Trait integration.
  • 5. Safety assessment of GM plants for food/feed safety
  • 6. Seed production and product release
  • 7. Stewardship
  • 8. Conclusion
  • Acknowledgment
  • References
  • Further reading
  • 7
  • Nicotianamine enhances zinc transport to seeds for biofortification
  • 1. Introduction
  • 1.1 Zinc deficiency among human populations
  • 1.2 Importance of Zn biofortification in rice
  • 2. The role of nicotianamine (NA) in plant Zn and Fe homeostasis
  • 2.1 NA as a precursor of phytosiderophore synthesis
  • 2.2 The role of NA as a Zn and Fe chelator
  • 2.3 The role of NA in long-distance Zn transport
  • 2.3.1 The role of NA in the symplastic pathway
  • 2.3.2 The role of NA in xylem Zn transport
  • 2.3.3 The role of NA in phloem Zn transport
  • 2.4 NA as a metal chelator for metal hyperaccumulation
  • 2.5 The role of NA in Zn homeostasis in Zn-deficient rice
  • 2.6 MAs produced from NA also contribute to Zn translocation in plants
  • 3. Zn and NA transporters involved in Zn xylem and phloem loading
  • 3.1 Roles of YSL transporters in NA-chelated Zn and Fe
  • 3.2 The roles of ZIF1 and ZIFL in Zn homeostasis
  • 3.3 ZIP and HMA transporters
  • 4. NAS genes as promising targets for Zn biofortification
  • 4.1 NA synthase gene overexpression enhances the seed Zn concentration and bioavailability
  • 4.2 Enhanced MAs production and NAS overexpression lead to efficient Zn biofortification
  • 4.3 Combined overexpression of NAS and other genes
  • 5. Future prospects of Zn biofortification by NA
  • 5.1 Zn concentration and genetic diversity among rice varieties
  • 5.2 Zn and Fe biofortification can be achieved in parallel
  • 5.3 Future perspectives
  • 6. Conclusion
  • References
  • 8
  • Zinc biofortification of rice by engineering metal transporter genes
  • 1. Introduction
  • 2. Zn transporters in rice plants
  • 2.1 Root Zn uptake.
  • 2.2 Long-distance transport (xylem and phloem)
  • 2.3 Zn transport to the seeds
  • 3. Zn biofortification through engineering expression of transporters
  • 3.1 Single gene strategies
  • 3.2 Combined genes strategies
  • 4. Concluding remarks and future directions
  • References
  • 9
  • Reducing cadmium content in zinc biofortified rice through genetic manipulation
  • 1. Introduction
  • 2. Source of Cd contamination
  • 3. Estimation of cadmium
  • 4. Genetic variations of Cd accumulation in rice
  • 5. Agronomic/crop management practices on Cd availability and accumulation
  • 6. Association between Zn and Cd on crop growth and development
  • 7. Current progress in low Cd high Zn biofortified rice
  • 7.1 Zn biofortification
  • 7.2 Field grown Zn biofortified rice and transgenic with high levels of Zn content
  • 8. Development of high Zn biofortified rice with low Cd
  • 8.1 Different routes to lower Cd content
  • 9. Genes, transporters, and chelators associated with Cd and Zn
  • 9.1 Genes involved in the transport of Cd and Zn
  • 9.2 Cd specific transporters
  • 9.3 Cd chelation and detoxification
  • 9.4 Vacuolar sequestration of Cd
  • 10. Transgenic and gene editing approach in low Cd high Zn biofortification
  • 11. Future perspective
  • References
  • Further reading
  • 10
  • Improving bioavailability of zinc in rice grains by reducing antinutrients through genetic engineering
  • 1. Phytic acid as an antinutrient
  • 1.1 Rice
  • 2. Managing Zn bioavailability through manipulating PA content
  • 2.1 The balances between Zn and PA content in rice
  • 2.2 Distribution of Zn and PA in the plant body
  • 2.3 Distribution of Zn and PA in the rice grain and effect on Zn bioavailability
  • 3. Discovery of genetic determinants
  • 3.1 Reverse-genetic approaches: discovery of PA biosynthesis by lpa mutants
  • 3.2 Genetic approaches: QTL and GWAS.