Neuro-urology research : a comprehensive overview /
**Selected for Doody's Core Titles® 2024 in Urology**Neuro-Urology Research: A Comprehensive Overview describes the current status of the neuro-urology field including the latest discoveries, explains in detail some of the neuroscience tools that can be used when studying the neural control of...
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| Format: | eBook |
| Language: | English |
| Published: |
[London] :
Academic Press,
2023.
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| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Front Cover
- Neuro-Urology Research
- Neuro-Urology Research
- Copyright
- Contents
- Contributors
- About the editor
- Preface
- Acknowledgments
- 1
- Neuro-urology research: a comprehensive overview
- Outline
- Chapter 1-Neuro-urology research: a comprehensive overview
- Chapter 2-Barrington's nucleus: a century of progress identifying neurons that control micturition
- Chapter 3-Voluntary versus reflex micturition control
- Chapter 4-The bladder as a readout in neuroscience research
- Chapter 5-How treatment of lower urinary tract symptoms can benefit from basic research
- Chapter 6- ``Translational effects of neuro-urology research on clinical practice''
- Patient population-specific lower urin ...
- Chapter 7-Effect of androgens and estrogens on bladder/lower urinary tract function
- Chapter 8-Transcriptomic identification of cell types in the lower urinary tract
- Chapter 9-Exploring urinary bladder neural circuitry through calcium imaging
- Chapter 10-The periaqueductal gray and control of bladder function
- Chapter 11-Impact of spinal neuromodulation on spinal neural networks controlling lower urinary tract function
- Chapter 12-Neural control of continence
- Introduction to neuro-urology
- The past and present of neuro-urology research
- Research questions and directions in the neuro-urology field
- Research topic 1: neuroanatomical sites for micturition behavior
- Research topic 2: neural circuits involved in bladder function
- Research topic 3: the functional brain-bladder connection
- Research topic 4: the "brain cause" of common lower urinary tract symptoms
- A quick guide to the "neuroscience toolbox"
- Transgenic animal models: knock-in and knockout (mice)
- Transgenic mice and CRISPR/Cas9 technology
- Cre-lox system and genetic tools
- Neural circuit tracing.
- Transcriptomics for identifying the gene expression profile of cells
- Spatially resolving gene expression profiles in intact biological samples
- Recording neural activity
- Fiber photometry during awake CMG
- Cell-specific manipulation of activity
- Optogenetic stimulation or inhibition of neuron activity
- Optogenetics 2.0 and special tools
- Chemogenetics for stimulating or inhibiting neuron activity
- Diphtheria toxin-mediated ablation and tetanus toxin-induced neuronal silencing
- Channelrhodopsin-assisted circuit mapping
- Bladder function readout that can be used with the described tools
- Micturition video thermography void spot assay
- Video cystometry
- Conclusions
- References
- I
- Neuroscience in urology research
- 2
- Barrington's nucleus: a century of progress identifying neurons that control micturition
- Introduction
- The micturition reflex
- Neuroanatomical landscape surrounding Barrington's nucleus
- Locus coeruleus
- Pontine central gray
- Pre-locus coeruleus
- Laterodorsal tegmental nucleus
- Mesencephalic nucleus of the trigeminal nerve and fourth ventricle
- Discovery and characterization of the neurons in Barrington's nucleus
- Neuroanatomic identification of Bar neurons
- Molecular characterization of Bar neurons
- Additional features of Bar neurons
- Human pontine tegmentum and micturition
- Efferent projections of Bar neurons
- Afferent projections to Bar neurons
- Conclusion
- References
- 3
- Voluntary versus reflex micturition control
- Introduction
- Neural circuits involved in reflex micturition control
- Peripheral nervous system
- Parasympathetic pathways
- Sympathetic pathways
- Somatic pathways
- Afferent pathways
- Intraspinal pathways
- Organization of storage reflexes
- Spinal reflex pathways
- Supraspinal pathways
- Organization of voiding reflexes.
- Spinobulbospinal reflex pathways
- Brainstem circuitry
- Role of PMC
- Properties of neurons in the PMC
- Role of the PAG
- Spinal micturition pathways
- Developmental changes in micturition pathways
- Reorganization of spinal micturition reflexes due to spinal cord legions
- Neural circuits involved in voluntary micturition control
- Cortical modulation of micturition
- Human imaging studies
- Animal studies
- Subcortical modulation of micturition
- Hypothalamus
- Basal ganglia, substantia nigra pars compacta, and ventral tegmental area
- Cerebellum
- Neurotransmitters in cortical and subcortical controls of micturition
- Glutamate
- Acetylcholine
- GABA and glycine
- Dopamine
- Serotonin (5-hydroxytryptamine)
- Stress-related peptides
- Conclusion
- References
- 4
- The bladder as a readout in neuroscience research
- Introduction
- Bladder function as a readout in basic neuroscience studies Electrical stimulation of the brain and measurement of bladder ...
- Pontine micturition centers
- The periaqueductal gray area
- PPN and rostral pontine areas affecting the micturition reflex
- Cerebellum
- Thalamus and hypothalamus
- Subthalamic nucleus
- Basal ganglia
- Higher (cortical) areas
- Recording of neural activity within the brain during physiological changes in bladder activity
- Optogenetics and pharmacological manipulation of brain circuits with measurement of bladder function
- Bladder function as a readout in clinical neuroscience including developmental, regenerative, and degenerative neuroscience
- Developmental neuroscience
- Spina bifida and tethered cord
- Postnatal emergence of continence in animals and humans
- Degenerative neuroscience
- Parkinson's disease
- Normal pressure hydrocephalus
- Multiple system atrophy
- Regenerative neuroscience
- Cauda equina/conus medullaris compression.
- The autonomic nervous system and bladder control
- The organization of autonomic pathways innervating the bladder
- The central autonomic network and the bladder
- The bladder as a readout in affective and social neuroscience: understanding the cognition of voiding
- Conclusion
- References
- Further reading
- II
- Fundamental and translational neuro-urology research
- 5
- How treatment of lower urinary tract symptoms can benefit from basic research
- Introduction
- Afferent bladder pathways
- Location of afferent neurons in lower urinary tract
- Pelvic and pudendal nerve afferents
- Hypogastric nerve afferents
- Spinal interneurons
- Role and properties of lower urinary tract afferent nerves
- Two types of afferent neurons
- Chemical properties of afferent nerves
- Afferent neurons in bladder reflexes
- Role of urothelium
- Neuronal afferents role in LUTS
- Neuronal afferents' role in painful bladder syndrome and interstitial cystitis
- Conclusion
- References
- 6
- "Translational effects of neuro-urology research on clinical practice"
- Patient population-specific lower urina ...
- Brain-bladder axis in health
- Nomenclature
- Localization-related symptoms in patients with neurological disease
- Neurological populations with frequent lower urinary tract dysfunction
- Dementia
- Stroke
- Parkinson's disease
- Multiple system atrophy
- Multiple sclerosis
- Spinal cord injury
- Spina bifida
- Cauda equina syndrome
- Idiopathic urinary retention: Fowler's syndrome and functional urological disorders
- Treatment options for patients with neurological disorders
- Treatment for storage dysfunction
- Behavioral treatment
- Antimuscarinic drugs
- Mirabegron
- Desmopressin
- Alpha-blockers
- Intravesical drug treatment
- Intravesical botulinum toxin
- Intravesical antimuscarinics
- Tibial neuromodulation.
- Sacral neuromodulation
- Treatment for voiding dysfunction
- Intermittent self-catheterization
- Surgical options
- Bladder augmentation
- Urinary diversion
- Bladder neck and urethral procedures
- Artificial urinary sphincter
- Urethral sling
- Urinary tract infections
- New investigation and treatment possibilities
- Lower urinary tract classifications and urinary biomarkers
- Deep brain stimulation
- Cannabinoids
- Prophylaxis for UTI bacteriophages
- Early tibial nerve stimulation after SCI
- Conclusion
- References
- 7
- Effect of androgens and estrogens on bladder/lower urinary tract function
- Sex differences in bladder histology
- Sex differences in anatomy and physiology
- Sex differences in histology
- Muscle and collagen
- Impact of testosterone and estradiol on smooth muscle physiology
- Smooth muscle
- Nerves
- Testosterone and estradiol in benign bladder diseases
- Lower urinary tract symptoms
- Aging and testosterone and estradiol
- LUTS, testosterone and estradiol, and innervation
- Muscle sensitivity
- Testosterone as a therapy for LUTS
- Stress incontinence
- Aging and T and E2
- Estrogens and innervation
- Estradiol as a therapy
- Overactive bladder
- TRPs in overactive bladder
- Environmental factors that mediate changes in testosterone and estradiol concentrations
- Diet
- Environmental chemical exposure
- Summary and conclusions
- References
- Further reading
- 8
- Transcriptomic identification of cell types in the lower urinary tract
- Bulk transcriptional profiling
- Example of bulk RNA sequencing protocol
- Single-cell transcriptional profiling
- Example of tissue digestion protocol
- Overview of single-cell RNA sequencing protocol
- Overview of bioinformatics
- Validation of cell type identification
- Spatial transcriptomics
- Conclusion
- References.
- III
- Neurobiological tools applied to neuro-urology research.