Integrated methods in protein biochemistry. Part A /
Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new funct...
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| Format: | eBook |
| Language: | English |
| Published: |
Cambridge, MA :
Academic Press,
2022.
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| Series: | Methods in enzymology ;
v. 675. |
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- 1. Engineering protein and DNA tools for creating DNA-dependent protein switches / Harsimranjit Sekhon, Jeung-Hoi Ha, and Stewart N. Loh
- 2. Reconstitution and biochemical studies of extended synaptotagmin-mediated lipid transport / Ruyue He, Chenlu Li, Yinghui Liu, and Haijia Yu
- 3. Synthesis of O-GlcNAcylated small heat shock proteins / Stuart P. Moon and Matthew R. Pratt
- 4. Druggable sites/pockets of the p53-DNAJA1 protein-protein interaction: In silico modeling and in vitro/in vivo validation / Danielle Jacobsen, Omar Bushara, Rama K. Mishra, Leyu Sun, Jie Liao, and Guang-Yu Yang
- 5. RAPIDS, a method for sub-compartmental identification of protein interactomes / Christina James, Christof Lenz, and Ralph H. Kehlenbach
- 6. Purification and biochemical characterization of the Rag GTPase heterodimer / Dylan D. Doxsey and Kuang Shen
- 7. Isolation of synthesized E. coli elongation complexes for solid-phase and solution-based in vitro transcription assays / Eric J. Strobel, Skyler L. Kelly, and Courtney E. Szyjka
- 8. Cellular experiments to study the inhibition of c-Myc/MAC heterodimerization / Ashutosh Singh, Shilpa Sharma, Praveen Kumar, and Neha Garg
- 9. Fluorogenic RNA aptamers to probe transcription initiation and co-transcriptional RNA folding by multi-subunit RNA polymerases / Yong-Heng Huang, Vilma Trapp, Oskari Puro, Janne J. Mäkinen, Mikko Metsä-Ketelä, Markus C. Wahl, and Georgiy A. Belogurov
- 10. Phosphorylation and subcellular localization of human phospholipase A1, DDHD1/PA-PLA1 / Atsushi Yamashita, Naoki Matsumoto, Yoko Nemoto-Sasaki, Saori Oka, Seisuke Arai, and Ikuo Wada
- 11. Elucidation of folding pathways of knotted proteins / Sarita Puri and Shang-Te Danny Hsu
- 12. Biophysical and structural characterizations of the effects of mutations on the structure-activity relationships of SARS-CoV-2 spike protein / Pei-Yu Yu, Tzu-Jing Yang, and Shang-Te Danny Hsu
- 13. A gel electrophoresis-based assay for measuring enzymatic RNA decapping activity / Yogeshwari Singh and Jeremy G. Bird
- 14. Nanoluciferase-based cell fusion assay for rapid and high-throughput assessment of SARS-CoV-2-neutralizing antibodies in patient samples / Max Meyrath, Martyna Szpakowska, Jean-Marc Plesseria, Olivia Domingues, Jérémie Langlet, Bernard Weber, Rejko Krüger, Markus Ollert, and Andy Chevigné
- 15. Linchpin-directed precise labeling of lysine in native proteins, purification, and analysis / Ankita Bal, Shivam Kumar Singh, Tanushree Kashyap, and Vishal Rai
- 16. In vitro and in cellular methods for determining the target protein SUMOylation / Sajeev T.K., Aswini Kumar Panda, Jyotsna Kawadkar, Divya Bindra, Pradyumna A. Joshi, Garima Joshi, Harsh Vardhan Singh, and Ram Kumar Mishra
- 17. Two-dimensional electrophoresis-cellular thermal shift assay (2DE-CETSA) for target identification of bioactive compounds / Makota Muroi and Hiroyuki Osada..