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220915s2023 enka ob 001 0 eng d |
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|a YDX
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|a 0128222786
|q electronic book
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|a 9780128222782
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|z 9780128222775
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|a 616.02774
|2 23
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|a TXAM
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| 245 |
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|a Phenotyping of human IPSC-derived neurons :
|b patient-driven research /
|c edited by Elizabeth D. Buttermore.
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| 264 |
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1 |
|a London ;
|a San Diego, CA :
|b Academic Press, an imprint of Elsevier,
|c [2023]
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| 300 |
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|a 1 online resource :
|b illustrations
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| 336 |
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|a text
|b txt
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|a computer
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|a online resource
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| 504 |
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|a Includes bibliographical references and index.
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|a Description based on online resource; title from digital title page (viewed on December 01, 2022).
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|a Front Cover -- PHENOTYPING OF HUMAN IPSC-DERIVED NEURONS -- PHENOTYPING OF HUMAN IPSC-DERIVED NEURONS: PATIENT-DRIVEN RESEARCH -- Copyright -- Dedication -- Contents -- Contributors -- I -- Best practices and considerations when designing a new project -- 1 -- iPSC culture: best practices from sample procurement to reprogramming and differentiation -- Facility setup -- Tissue culture room design -- Tissue culture equipment -- Primary sample collection -- Somatic cells -- Quality control of somatic cells -- Reprogramming -- Pros and cons of each method -- Episomal vector transfection -- Sendai virus transduction -- mRNA reprogramming method -- iPSC line characterization -- Sterility -- Pluripotency -- Transgene elimination -- Identity -- Genetic stability -- Best practices prior to differentiation -- Cell banking -- Culturing conditions -- Differentiation -- Experimental design -- Cell line selection -- Differentiation protocol selection -- Best practices during differentiation -- References -- 2 -- Phenotypic assay development with iPSC-derived neurons: technical considerations from plating to analysis -- Introduction -- Establishing optimal conditions for phenotyping iPSC-derived neurons -- Differentiation protocol considerations -- Coating substrates -- High content imaging (HCI) -- Functional analysis -- Multi-electrode array (MEA) recording -- Calcium imaging -- Patch clamping -- Live imaging -- Fluorescent microplate assays -- Assay development for screening -- Conclusion -- References -- 3 -- Derivation of cortical interneurons from human pluripotent stem cells to model neurodevelopmental disorders -- Introduction -- Development of the human cortex -- Modeling human cortical interneuron development in vitro -- The development of protocols for cortical interneurons from human pluripotent stem cells (hPSCs) to model neurodevelopmenta.
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| 505 |
8 |
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|a A protocol for cortical interneuron derivation from human pluripotent stem cells (hPSCs) -- Equipment and supplies -- Reagents -- Preparation of reagents -- Accutase cell detachment solution -- B-27 supplement (50×) minus vitamin A -- Preparing matrigel -- Coating tissue culture plates with Matrigel -- Coating tissue culture plates with Matrigel-Laminin -- Small molecule preparation -- Media composition -- Protocol -- Specification of cortical interneuron progenitors from hPSCs -- Maintenance and expansion of cIN NPCs -- Cryopreservation of cIN neural progenitor cells -- Revival and maintenance of cryopreserved cIN neural progenitor cells -- Interneuron differentiation and maturation from cIN neural progenitor cells -- Enrichment and purification of cIN neural progenitor cells and neurons -- Enrichment for post-mitotic cINs with neural rosette selection reagent -- Purification of post-mitotic cINs with NCAM bead selection -- Critical steps and troubleshooting -- Cellular phenotyping of hPSC-derived cINs -- Using immunocytochemistry to benchmark hPSC-derived cINs and to assess NDD-related alterations of neurodevelopment -- Morphometric analysis of neurite extension and length -- Neuronal migration assay -- Measurement of synaptic puncta -- Alternate protocol for derivation of cIN NPCs from hPSCs -- Alternate protocol for differentiation of cIN NPCs into interneurons -- Acknowledgments -- References -- 4 -- Development of transcription factor-based strategies for neuronal differentiation from pluripotent stem cells -- Introduction -- Neuron differentiation driven by transcription factors -- Dopaminergic (DA) neurons -- Glutamatergic neurons -- GABAergic neurons -- Cholinergic motor neurons -- Retinal ganglion cells -- Glia: astrocytes, oligodendrocytes, and microglia.
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| 505 |
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|a Transcription factor-driven differentiation: considerations when designing a new protocol -- Design a cocktail of transcription factors -- Transcription factor delivery -- Genome integrating vectors -- Non-genome integrating viral vectors -- Synthetic mRNA -- Summary and future directions -- Acknowledgement -- References -- 5 -- Differentiation of Purkinje cells from pluripotent stem cells for disease phenotyping in vitro -- Development of the cerebellum -- Differentiation of pluripotent stem cells into Purkinje cells -- Cerebellar organoids derived from iPSCs and ESCs in 3D cultures -- Human iPSC- and ESC-derived Purkinje cell differentiation in 2D co-cultures with mouse cerebellar cells -- Functional characterization of human pluripotent stem cell-derived Purkinje cells in vitro and in vivo -- Challenges in the differentiation of human Purkinje cells in 2D- and 3D-cell cultures -- Disease phenotyping of Purkinje cells -- Purkinje cells in cerebellar ataxia -- Mouse Purkinje cell models of cerebellar ataxia -- Human iPSC-derived NPCs and Purkinje cells in cerebellar ataxia -- Purkinje cells in Tuberous Sclerosis Complex (TSC) -- Mouse Purkinje cell models of TSC -- TSC patient iPSC-derived Purkinje cells -- Future perspectives for stem cell-derived Purkinje cells in translational medicine -- Cell transplantation for treatment of cerebellar degeneration -- Drug screening with pluripotent stem cell-derived Purkinje cells -- Acknowledgments -- References -- 6 -- Brain organoids: models of cell type diversity, connectivity, and disease phenotypes -- Introduction -- Cerebral organoids -- Human corticogenesis overview -- Organoid differentiation overview -- Fidelity of hCO cell types and organization -- Other brain region specific organoids -- Neuronal activity and connectivity -- Synaptic activity -- Connectivity of neuronal organoids -- Non-neuronal cells.
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| 505 |
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|a Astrocytes -- Oligodendrocytes -- Microglia -- Vascularization/nutrient distribution -- Summary of non-neuronal cells -- Use of models in disease -- Microcephaly modeling with hCOs -- ASD modeling with hCOs -- Molecularly defined ASD -- Idiopathic ASD -- Limitations of hCO modeling for CNS disorders -- Reproducibility -- Sources of variability in organoid model systems -- Addressing reproducibility -- Conclusions and future directions -- References -- II -- The use of iPSC-derived neurons to study neurological disorders -- 7 -- Human models as new tools for drug development and precision medicine -- Introduction -- Drug development pipeline -- Human models as a screening tool for personalized precision medicine -- Monolayer models -- Organoids -- Organ-on-chip platforms -- Conclusion -- References -- 8 -- Use of cerebral organoids to model environmental and gene x environment interactions in the developing fetus an ... -- Introduction -- Maternal immune activation -- Cerebral organoids as a model system to study MIA and neuroinflammation -- Cerebral organoids as a model system to study infectious diseases that cause neurodevelopmental disorders -- Zika virus -- SARS-CoV-2 -- Human immunodeficiency virus (HIV) -- Toxoplasmosis -- Cytomegalovirus (CMV) -- Herpes simplex virus (HSV) -- Cerebral organoids and cellular stress -- Heat shock -- Fetal alcohol syndrome -- Cerebral organoids to model neurodegenerative disorders -- Alzheimer's disease (AD) -- Cerebral organoids in familial AD -- Modeling sporadic AD -- Cerebral organoids for drug development in AD -- Modeling Parkinson Disease using organoid cultures -- Conclusion -- References -- 9 -- iPSC-derived models of autism: Tools for patient phenotyping and assay-based drug discovery -- Introduction -- Syndromic autisms -- Fragile X syndrome -- Rett syndrome -- FOXG1 deletion syndrome -- Tuberous sclerosis.
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| 505 |
8 |
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|a Pheland McDermid syndrome -- Prader-Willi and Angelman syndromes -- Timothy syndrome -- iPSC studies to model ASDs in vitro -- iPSC studies focused on syndromic and sporadic autisms -- iPSC studies focusing on sporadic non-syndromic autism -- Data collected by studies focused on iPSCs from idiopathic autism -- Gene expression profiling -- Concordances in gene expression profiles obtained from studies on iPSC-derived cells and post-mortem brain tissue from idio ... -- Morphological and electrophysiological properties in iPSC-derived neurons from patients with idiopathic autism -- Similar phenotypes between iPSC-derived neurons from patients with sporadic or syndromic autisms and idiopathic autism -- 3D models of ASDs-a focus on organoids, spheroids, and assembloids -- The use of iPSCs to develop assays and novel therapies that can be translated to the clinic for ASD -- Limitations for using iPSC-derived neurons in drug screening platforms -- Quality control testing -- Automation challenges -- Cost -- Small "n" -- Epigenetic memory -- Well-to-well variability -- Variability within cell lines -- Variability across differentiation batches -- Disease modeling -- Screening of simple phenotypes -- The use of iPSC-derived neurons for personalized medicine -- Conclusions -- References -- 10 -- Probing the electrophysiological properties of patient-derived neurons across neurodevelopmental disorders -- Induced pluripotent stem cells and modeling brain disorders -- Progressing from gene discovery to functional gene groupings to pathophysiology -- Neuronal networks represent a logical level for the manifestation of NDDs -- Micro-electrode arrays as a scalable high-throughput functional assay -- Phenotyping NDD patient-derived neurons using MEA recordings -- Fragile X and Rett syndrome -- Kleefstra syndrome -- Neuronal networks as converging pathways?.
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| 520 |
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|a "Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research examines the steps in a preclinical pipeline that utilizes iPSC-derived neuronal technology to better understand neurological disorders and identify novel therapeutics, also providing considerations and best practices. By presenting example projects that identify phenotypes and mechanisms relevant to autism spectrum disorder and epilepsy, this book allows readers to understand what considerations are important to assess at the start of project design. Sections address reproducibility issues and advances in technology at each stage of the pipeline and provide suggestions for improvement. From patient sample collection and proper controls to neuronal differentiation, phenotyping, screening, and considerations for moving to the clinic, these detailed descriptions of each stage of the pipeline will help everyone, regardless of stage in the pipeline."--
|c Title details screen.
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| 650 |
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|a Stem cells
|x Research.
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| 650 |
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|a Phenotype.
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| 650 |
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|a Nervous system
|x Diseases
|x Treatment.
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| 650 |
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|a Phenotype
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| 650 |
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|a Cellules souches
|x Recherche.
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| 650 |
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|a Phénotypes.
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| 650 |
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|a Système nerveux
|x Maladies
|x Traitement.
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| 650 |
|
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|a Nervous system
|x Diseases
|x Treatment
|2 fast
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| 650 |
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7 |
|a Phenotype
|2 fast
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| 650 |
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7 |
|a Stem cells
|x Research
|2 fast
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| 655 |
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7 |
|a Electronic books.
|2 local
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| 710 |
2 |
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|a ScienceDirect (Online service)
|
| 776 |
0 |
8 |
|i Print version:
|z 0128222778
|z 9780128222775
|w (OCoLC)1302575565
|
| 856 |
4 |
0 |
|u http://proxy.library.tamu.edu/login?url=https://www.sciencedirect.com/science/book/9780128222775
|z Connect to the full text of this electronic book
|t 0
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| 955 |
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|a Elsevier ScienceDirect 2026-2027
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| 994 |
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|a 92
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| 952 |
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|a Texas A&M University
|b College Station
|c Electronic Resources
|s www_evans
|d Available Online
|t 0
|e QH588.S83 P54 2023
|h Library of Congress classification
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| 998 |
f |
f |
|a QH588.S83 P54 2023
|t 0
|l Available Online
|