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MicroRNA in human malignancies /

MicroRNA in Human Malignancies offers a deep overview of the role and translational significance of miRNAs in the development of cancer and other malignancies.The book establishes the foundations of the field by covering essential mechanisms and the translational potential of miRNAs in the field of...

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Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Negrini, Massimo, Calin, George A. (George Adrian), Croce, Carlo
Format: eBook
Language:English
Published: London : Elsevier, 2022.
Subjects:
MicroRNA.
MicroRNAs
MicroARN.
MicroRNA
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Intro
  • MicroRNA in Human Malignancies
  • Copyright
  • Contents
  • Contributors
  • Section A: miRNAs involvement in basic mechanisms of cancer development
  • Chapter 1 Overview on miRNA classification, biogenesis, and functions
  • Introduction
  • MicroRNA and the complex biological system of gene expression
  • MicroRNA biogenesis, nomenclature, and classification
  • Origins of the canonical primary miRNA transcript
  • From canonical primary miRNA to mature miRNA
  • Noncanonical miRNA biogenesis and isomiRs
  • Nomenclature and classification
  • Mechanisms of miRNA-mediated gene regulation
  • Posttranscriptional regulation of mRNA stability and translation
  • Models of miRNA-mediated gene regulation within the nucleus
  • The dynamics of miRNA-mediated gene regulation
  • MicroRNA abundance and turnover
  • Concluding remarks
  • References
  • Chapter 2 microRNA in cancer: An overview
  • The dawn of miRNAs in human biology
  • First reported miRNA cancer-associated alterations
  • The age of discovery: Microarrays and miRNA cancer signatures
  • Functional classification of miRNA: The line between tumor suppressor and oncomiRs
  • Development of miRNA as biomarkers
  • The transition of miRNA into clinics
  • Conclusion and future perspectives
  • References
  • Chapter 3 miR-15/16 in human malignancies
  • 13q deletions in CLL and miR-15/16
  • Other mechanisms of miR-15/16 dysregulation
  • Interplay between ROR1, Bcl2, and miR-15/16 in CLL
  • miR-15/16 in mice
  • miR-15/16 in human AML and MDS
  • miR-15/16 in solid cancers
  • References
  • Chapter 4 microRNAs and tumor suppressor p53 regulation
  • p53 and its signaling pathway
  • GOF mutant p53 in cancer
  • miRNAs regulated by wild-type p53
  • miRNAs that directly regulate p53
  • miRNAs that indirectly regulate p53
  • miRNAs regulated by GOF mutp53
  • Conclusion
  • References.
  • Chapter 5 MicroRNA involvement in invasion and metastasis
  • Introduction-The process of metastasis
  • Direct regulation of metastasis
  • Metastasis-promoting miRNAs
  • Metastasis-Suppressing miRNAs
  • Indirect regulation of metastasis
  • Regulation of miRNA biosynthesis
  • RNA-RNA interaction-based miRNA regulation
  • Translational application of metastasis-associated miRNAs
  • Noninvasive biomarkers
  • miRNAs as therapeutics
  • Conclusions and perspectives
  • Acknowledgment
  • References
  • Chapter 6 microRNAs and metabolism
  • Introduction
  • Trafficking and consumption of glucose
  • NcRNAs regulate glucose trafficking in cancer cells by altering GLUT levels
  • NcRNAs regulate key glycolytic enzymes
  • Branched pathways of glycolysis
  • NcRNAs can influence glucose metabolism by regulating cancer-associated signaling pathways
  • PI3K/Akt/mTOR and HIF-1 signaling pathway
  • p53 signaling pathway
  • Conclusion and future perspectives
  • References
  • Chapter 7 microRNAs in inflammation processes
  • Introduction
  • MiRNA biogenesis and functions
  • Inflammation and mechanism of activation
  • miRNAs in inflammatory disease
  • Chronic respiratory diseases
  • Cystic fibrosis (CF)
  • Chronic obstructive pulmonary disease (COPD)
  • Asthma
  • Cardiovascular diseases
  • Atherosclerosis
  • Myocardial infarction (MI) and ischemia reperfusion injury (MIRI)
  • Autoimmune diseases
  • Systemic lupus erythematosus (SLE)
  • Rheumatoid arthritis (RA)
  • Multiple sclerosis (MS)
  • Cancer
  • Colorectal cancer (CRC)
  • Lung cancer (LC)
  • Prostate cancer (PC)
  • Conclusion
  • References
  • Section B: miRNAs methodologies
  • Chapter 8 Wet-lab methods for miRNA analysis
  • Introduction
  • Methods for miRNA discovery and detection
  • Northern blot
  • Hybridization microarrays
  • Quantitative PCR
  • Next-generation sequencing
  • Functional miRNA analysis.
  • Classical target validation methods: Reporter genes and RISC immunoprecipitation
  • High-throughput methods for target identification
  • Functional validation
  • Final remarks
  • Acknowledgments
  • References
  • Chapter 9 Bioinformatics utilities, web resources and integrative strategies for the analysis of miRNA regulatory networks
  • Introduction
  • Target prediction algorithms
  • Single predictors
  • Multiple predictors
  • In silico functional assessment of miRNAs: Tips and tricks
  • Functional analysis of miRNAs associated with survival time in lung adenocarcinoma: A test case
  • Conclusions
  • Acknowledgments
  • References
  • Chapter 10 Computational resources for analysis of miRNA targetome
  • Introduction
  • miRNA target databases
  • miRNA target databases for human
  • miRNA target databases for other species
  • miRNA algorithms
  • miRNA target prediction for humans
  • miRNA target prediction for other species
  • miRNA target prediction for plants
  • Conclusion
  • References
  • Chapter 11 miRNA bioinformatics and pathway analysis
  • The microRNA synergy
  • Role of microRNAs in biological signaling pathways
  • Pathways: The manually curated maps of the cell interactome
  • The miRNA interactome
  • miRNA pathway analysis methods
  • ORA on miRNA targets
  • FCS on miRNA targets
  • Topological approaches of pathway analysis with miRNAs
  • Computational tools for miRNA pathway analyses
  • Conclusions
  • Funding
  • References
  • Section C: miRNAs involvement in therapeutics/theranostics
  • Chapter 12 Opportunities of miRNAs in cancer therapeutics
  • Introduction
  • MicroRNAs in cancer. OncomiRs and tumor-suppressor miRNAs
  • Alterations in miRNAs and their appeal for cancer therapy
  • MicroRNA-based drug design
  • Choosing the therapeutic strategy: miRNA mimics, antagomiRs, and more
  • Chemical modifications of RNA-based drugs.
  • Delivery of RNA-based drugs
  • Chemical modifications
  • Liposomes, micelles, and exosomes
  • Nanoparticles
  • Viral vectors
  • Bacteria-based vectors (EDV nanocells)
  • MicroRNA-based therapeutics in the current clinic
  • MRX34
  • Cobomarsen
  • TargomiRs
  • Remlarsen
  • Other preclinical studies
  • The future of miRNAs in the clinic
  • Current challenges in the use of miRNAs
  • Quick degradation in the bloodstream
  • Extrahepatic delivery to tumor site
  • Limited tissue penetration
  • Endocytosis and endosome entrapment
  • Off-targets and side effects
  • Other challenges
  • Future perspectives
  • References
  • Section D: miRNAs involvement in human cancer: Pathophysiology and translational opportunities
  • Chapter 13 Pathophysiology roles and translational opportunities of miRNAs in acute leukemias
  • Introduction
  • MiRNAs involved in the pathogenesis of AML
  • miRNAs as biomarkers
  • miRNAs in chemoresistance
  • miRNAs as therapeutic targets
  • Role of miR-15/16 clusters in AML pathogenesis
  • Conclusions
  • References
  • Chapter 14 Pathophysiology roles and translational opportunities of miRNAs in CLL
  • MicroRNAs and the cellular origin of CLL cells
  • MicroRNAs and CLL genetics
  • microRNAs in proliferation and survival of CLL cells
  • microRNAs and BCR signaling
  • MicroRNAs and microenvironment
  • MicroRNAs and apoptosis
  • Translational opportunities
  • References
  • Chapter 15 Pathophysiology roles and translational opportunities of miRNAs in lymphoma
  • Summary
  • miRNAs and pathophysiology
  • miRNAs regulate the expression of genes that promote B-cell differentiation
  • Lymphomagenesis
  • DLBCL and miRNA
  • MZL and miRNA
  • Diagnosis
  • Prognosis
  • References
  • Chapter 16 Pathophysiology roles and translational opportunities of miRNAs in breast cancer
  • Breast physiology and pathology
  • Breast physiology
  • Breast pathology.