Carbohydrate analysis by modern liquid phase separation techniques /

Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates.

Bibliographic Details
Corporate Author:	ScienceDirect (Online service)
Other Authors:	El Rassi, Ziad (Editor)
Format:	eBook
Language:	English
Published:	Amsterdam : Elsevier, 2021.
Edition:	Second edition.
Subjects:	Carbohydrates > Analysis. Separation (Technology) Glucides > Analyse. Séparation (Technologie) Carbohydrates > Analysis Electronic books.
Online Access:	Connect to the full text of this electronic book

Table of Contents:

Intro
Carbohydrate Analysis by Modern Liquid Phase Separation Techniques
Copyright
Contents
Contributors
Preface
Chapter 1: Derivatization of carbohydrates for analysis by liquid chromatography and capillary electrophoresis
1 Introduction
2 General considerations for carbohydrate derivatization
3 Carbohydrate derivatization for detection in HPLC
4 Carbohydrate derivatization for detection in CE
5 Conclusion and future prospects
References
Chapter 2: Reversed-phase and hydrophobic interaction chromatography of carbohydrates and glycoconjugates
1 Introduction
2 Basic theoretical concepts-An overview
3 Chromatographic systems, methodologies, and applications
3.1 Reversed-phase chromatography
3.1.1 Stationary phase
3.1.2 Mobile phase
3.1.3 Some general aspects of RPC of carbohydrates and selected applications
3.1.3.1 Underivatized saccharides
3.1.3.2 Derivatized saccharides
3.1.3.3 Glycopeptides
3.1.3.4 Glycoproteins
3.2 Ion-pair reversed-phase chromatography
3.3 Hydrophobic interaction chromatography
3.3.1 Stationary phase
3.3.2 Mobile phase
3.3.3 Some general aspects of HIC of glycoproteins and selected applications
4 Conclusions
Acknowledgment
References
Chapter 3: HPLC of carbohydrates with cation-exchange silica and resin-based stationary phases
1 Introduction
2 Separation of carbohydrates on cation-exchange stationary phases
2.1 Silica-based cation-exchange stationary phases
2.2 Polymer-based cation-exchange stationary phases
2.2.1 Some basic characteristics of polymer-based cation-exchange stationary phases
2.2.2 Coupling of cation-exchange columns with different counter-ions
2.3 Separation mechanisms
2.3.1 Ion exchange
2.3.2 Ion exclusion
2.3.3 Size exclusion.
2.3.4 Ion-moderated partitioning chromatography and ligand exchange
2.3.5 Interactions with sulfonate groups
2.3.6 Interactions with the support matrix
2.4 Variables affecting the separation of carbohydrates on cation exchangers
2.4.1 Particle size
2.4.2 Cross-linking
2.4.3 Column temperature
2.4.4 Eluent composition
2.4.5 Methods for the detection of carbohydrates
3 Applications
3.1 Cation-exchange chromatography columns and suppliers
References
Chapter 4: High-performance anion-exchange chromatography with pulsed amperometric detection for carbohydrate and glycocon ...
1 Introduction
2 HPAEC-PAD in 2020
3 HPAEC-PAD basics
4 HPAEC columns
5 Developments in pulsed amperometric detection
6 Updated and new HPAEC-PAD applications
6.1 Monosaccharide analysis
6.2 Sialic acid analysis
6.3 Glycoprotein oligosaccharide profiling
6.4 Separation of milk oligosaccharides
6.5 Determination of carbohydrates in urine and serum
6.6 Determination of carbohydrates in atmospheric aerosols
6.7 Glycoconjugate vaccine analysis
6.8 HPAEC-PAD applied to biofuels research and development
6.9 HPAEC-PAD applications to plant carbohydrates and foods
6.10 HPAEC-PAD standard methods
7 Conclusion
Acknowledgments
References
Chapter 5 Hydrophilic interaction liquid chromatography-mass spectrometry for the characterization of glycoproteins at the ...
1 Introduction
2 Protein glycosylation
3 Glycoprofiling approaches
4 HILIC-MS of glycoproteins: Conditions and practicalities
4.1 HILIC retention mechanism
4.2 Experimental parameters impacting glycoprofiling by HILIC-MS
4.2.1 Glycan composition and relative molecular weight
4.2.2 HILIC stationary phase
4.2.2.1 Stationary phase chemistry
4.2.2.2 HILIC particle pore size
4.2.3 Mobile phase.
3.1.3.3 Indirect UV
3.2 Miscellaneous detection of carbohydrates
3.2.1 Dynamically labeled carbohydrates
3.2.2 Online preconcentration
3.2.3 Chemiluminescence and FTIR
3.2.4 Quantum dot-based fluorescence resonance energy transfer
3.2.5 Spectrophotometric determination of glucose via its enzyme degradation products
3.2.6 Refractive index detection
4 Separation approaches and selected applications
4.1 Monosaccharides
4.1.1 Underivatized monosaccharides
4.1.2 Derivatized monosaccharides
4.2 Oligosaccharides
4.2.1 Underivatized oligosaccharides
4.2.2 Derivatized oligosaccharides
4.2.2.1 Simple oligosaccharides
4.2.2.2 Homologous oligosaccharides
4.2.2.3 Applications involving linear and branched oligosaccharides derived from plants
4.2.2.4 Glycolipid-derived oligosaccharides
4.3 Polysaccharides
4.3.1 Underivatized polysaccharides
4.3.2 Dynamically labeled and derivatized polysaccharides
5 Capillary electrochromatography
6 Conclusions
Acknowledgments
References
Chapter 8: LC-MS/MS in glycomics and glycoproteomics analyses
1 Introduction
1.1 Glycosylation
1.2 The importance of glycosylation
1.3 Significance of glycan and glycopeptide isomers
1.4 Technical challenges of glycan and glycopeptide analysis
2 Current liquid phase techniques used for isomeric separation of glycans
2.1 Glycan derivatization used for isomeric separation
2.1.1 Derivatization of reducing ends
2.1.2 Permethylation of glycans
2.1.3 Linkage-specific derivatization of sialic acids
2.1.3.1 Amidation
2.1.3.2 Esterification
2.2 Separation methods for glycans
2.2.1 Liquid phase separation of glycans
2.2.1.1 Reversed-phase liquid chromatography
2.2.1.2 Hydrophilic interaction liquid chromatography
2.2.1.3 Porous graphitized carbon chromatography.
2.2.2 Capillary electrophoresis of glycans
2.2.2.1 Capillary zone electrophoresis in isomeric glycomic studies
2.2.2.2 Microfluidics capillary electrophoresis in isomeric glycomic studies
2.2.2.3 DNA analyzer in isomeric glycomic studies
3 Current techniques used for isomeric separation of glycopeptides
3.1 Separation methods for glycopeptides
3.1.1 Liquid phase separation of glycopeptides
3.1.1.1 Reversed-phase liquid chromatography
3.1.1.2 Hydrophilic interaction liquid chromatography
3.1.1.3 Porous graphitized carbon chromatography
3.1.2 Capillary electrophoresis of glycopeptides
3.1.2.1 Capillary zone electrophoresis
3.1.2.2 Capillary array electrophoresis chips
4 Software tools for glycomics and glycoproteomics
4.1 Software tools for glycomics
4.2 Software tools for glycoproteomics
5 Concluding remarks
Acknowledgments
References
Chapter 9: Capillary electrophoresis-mass spectrometry of carbohydrates and glycoconjugates
1 Introduction
2 Interfacing capillary electrophoresis with mass spectrometry
2.1 Fundamental aspects
2.2 Interface designs
3 Mono-, oligo-, and polysaccharides by CE-MS
3.1 Monosaccharides
3.2 Oligosaccharides and polysaccharides
4 Glycoconjugates
4.1 Lipid-based glycoconjugates
4.1.1 Lipopolysaccharides
4.1.2 Glycosphingolipids
4.2 Glycoproteins
4.2.1 The bottom-up approach
4.2.2 CE-MS of intact glycoproteins
4.2.3 Glycans by CE-MS
References
Chapter 10: Modern techniques for separation, mass spectrometric detection, and characterization of glycolipids
1 Introduction
2 Methods for extraction and purification
3 Liquid- and thin-layer chromatography in off- and on-line coupling to mass spectrometry
4 Capillary zone electrophoresis coupled to mass spectrometry
4.1 Off-line CZE ESI MS
4.2 On-line CZE ESI MS.