CRISPR and RNAi systems : nanobiotechnology approaches to plant breeding and protection /

CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted mo...

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Bibliographic Details
Corporate Author:	ScienceDirect (Online service)
Other Authors:	Abd-Elsalam, Kamel A., Lim, Ki-Taek
Format:	eBook
Language:	English
Published:	Amsterdam : Elsevier, 2021.
Series:	Nanobiotechnology for plant protection
Subjects:	Plant biotechnology. Crops > Genetic engineering. CRISPR-associated protein 9 > Biotechnology. RNA interference. Plant breeding. Plants, Protection of. Plantes > Biotechnologie. Cultures > Génie génétique. Protéine-9 associée à CRISPR > Biotechnologie. Interférence ARN. Plantes > Amélioration. Plantes > Protection. Crops > Genetic engineering Plant biotechnology Plant breeding Plants, Protection of RNA interference Electronic books.
Online Access:	Connect to the full text of this electronic book

Table of Contents:

Front Cover
CRISPR and RNAi Systems
Copyright Page
Contents
List of contributors
Series preface
Preface
1 Can CRISPRized crops save the global food supply?
1.1 Introduction
1.2 Gene editing techniques
1.3 RNAi and CRISPR systems for plant breeding and protection: where are we now?
1.3.1 Improving yield and quality in crops
1.3.2 Biotic and abiotic stress resistance
1.3.3 Speed breeding programs in plants
1.4 What are future perspectives?
1.5 Conclusion
References
2 Targeted genome engineering for insects control
2.1 Introduction
2.1.1 RNAi in insects
2.1.2 Prerequisites for RNAi response
2.1.3 Variation in RNAi response
2.1.4 ORDER specific RNAi applications
2.1.5 Pros and cons of RNAi-mediated insect control strategies
2.2 CRISPR/Cas9
2.2.1 CRISPR-Cas9 sex-ratio distortion and sterile insect technique
2.2.2 Potential targets for CRISPR system in insects
2.3 Conclusion and future prospects
References
3 CRISPR/Cas9 regulations in plant science
3.1 Introduction
3.2 Ethical concerns for CRISPR-based editing system
3.3 Biosafety concerns for genomic manipulated crops
3.4 Global regulations of CRISPR edit crops
3.4.1 The United States regulation policies for genome edit crops
3.4.2 Canada regulation policies for genome edit crops
3.4.3 European Union regulation policies for genome edit crops
3.4.4 China regulation policies for genome edit crops
3.4.5 Pakistan regulation policies for genome edit crops
3.4.6 India regulation policies for genome edit crops
3.4.7 Australia regulation policies for genome edit crops
3.4.8 Japan regulation policies for genome edit crops
3.4.9 New Zealand regulation policies for genome edit crops
3.4.10 Brazil regulation policies for genome edit crops
3.5 Conclusion and future outlook.
3.6 Conflict of interest
References
4 Are CRISPR/Cas9 and RNA interference-based new technologies to relocate crop pesticides?
4.1 Introduction
4.2 Conventional pesticides: present status and challenges
4.3 Advancement in green revolution: the RNAi toolkit
4.4 Advantages and disadvantages of RNAi-based methods
4.5 Advantages of CRISPR/Cas9-based systems
4.6 Conclusions and future prospects
Acknowledgments
References
Further reading
5 CRISPR-Cas epigenome editing: improving crop resistance to pathogens
5.1 Introduction
5.1.1 A brief history of CRISPR/Cas
5.1.2 CRISPR/Cas9-based genome editing
5.2 Applications of CRISPR/Cas9
5.2.1 Re-engineering Cas9 for genome editing
5.2.1.1 Double nicking CRISPR/Cas9
5.2.1.2 CRISPRi (CRISPR interference)
5.2.1.3 CRISPRa (CRISPR activation)
5.2.1.4 CRISPR I/O (input/output) gene regulation
5.2.1.5 CRISPR epigenome editing
5.2.1.6 CRISPR base editing
5.2.1.7 CRISPR prime editing
5.3 CRISPR/Cas12
5.4 CRISPR/Cas13 RNA editing
5.5 CRISPR/Cas14
5.6 Delivery of CRISPR/Cas system for (epi)genome editing
5.6.1 Virus-induced gene editing and viral delivery for CRISPR/Cas systems
5.6.2 Agrobacterium-mediated T-DNA transformation
5.6.3 PEG transformation
5.6.4 Direct delivery of ribonucleotide protein complexes
5.7 Cisgenic, intragenic, transgenic or edited plants
5.8 Epigenome editing
5.8.1 Targeted epigenetic regulation
5.8.2 Crop disease resistance
5.8.3 Limitations to epigenome editing
5.9 Summary and future directions
Acknowledgments
References
6 CRISPR/Cas system for the development of disease resistance in horticulture crops
6.1 Introduction
6.2 Bacterial resistance
6.2.1 Citrus canker
6.2.2 Fire blight
6.3 Fungal resistance
6.3.1 Powdery mildew
6.3.2 Gray mold
6.3.3 Black pod.
6.4 Virus resistance
6.4.1 RNA viruses
6.4.2 DNA viruses
6.5 Concluding remarks
References
7 CRISPR and RNAi technology for crop improvements in the developing countries
7.1 Introduction
7.2 Conventional breeding for crop improvements
7.3 RNAi technology: an overview
7.3.1 RNAi technology for crop improvements
7.3.1.1 Enhancement in biotic stress tolerance/resistance
7.3.1.2 Enhancement in abiotic stress tolerance/resistance
7.3.1.3 Engineering of seedless fruits
7.3.1.4 Enhancement of nutritional value
7.3.1.5 Induction of male sterility/heterosis
7.4 CRISPR technology for crop improvements: an overview
7.4.1 CRISPR technology for the development of biotic stress resistance
7.4.2 CRISPR technology for the development of abiotic stress resistance
7.4.3 CRISPR technology for nutritional modifications in crop
7.5 Crop improvements: examples from developing countries
7.5.1 China
7.5.2 India
7.5.3 Pakistan
7.5.4 Bangladesh
7.5.5 Africa
7.6 Conclusion and prospects
References
8 RNA interference and CRISPR/Cas9 applications for virus resistance
8.1 Introduction
8.2 Control of viral diseases using RNA interference approaches
8.3 Control of viral diseases using CRISPR/Cas technology
8.4 CRISPR/Cas genome editing against DNA viruses
8.5 CRISPR/Cas genome editing against RNA viruses
8.6 Production of foreign DNA-free virus-resistant plants by CRISPR/Cas
8.7 RNA interference versus CRISPR/Cas strategies
8.8 Conclusion
References
9 Current trends and recent progress of genetic engineering in genus Phytophthora using CRISPR systems
9.1 Introduction
9.2 Common diseases of crops caused by Phytophthora
9.3 Genome editing approaches
9.4 CRISPR-Cas systems for Phytophthora
9.5 Applications of CRISPR-Cas in genetic engineering of Phytophthora.
9.6 Challenges of CRISPR-Cas in Phytophthora
9.7 CRISPR-Cas based databases and bioinformatics tools for Phytophthora
9.8 Conclusion and future prospects
Acknowledgment
References
10 CRISPR/Cas9 and Cas13a systems: a promising tool for plant breeding and plant defence
10.1 Introduction
10.2 CRISPR/Cas technology and engineering plant resistance to viruses
10.3 Targeting plant DNA viruses using CRISPR/Cas9
10.4 Targeting RNA viruses using CRISPR/Cas13 and FnCas9
10.4.1 Direct interference of viral RNA genomes
10.4.2 Interference of plant host factors aiding viral infection
10.4.3 Advantages of genome editing technologies for breeding virus resistance
10.4.4 Caveats of employing the CRISPR/Cas technology to engineer resistance to plant viruses
10.4.4.1 Overcoming the caveats of the CRISPR/Cas systems
10.4.5 Future directions of genome editing to protect crops from viruses
10.5 CRISPR technology for plant improvement
10.5.1 Rice
10.5.2 Wheat
10.5.3 Cotton
10.5.4 Maize
10.5.5 Soya bean
10.5.6 Tomato
10.5.7 Potato
10.5.8 Citrus
10.5.9 Apples
10.6 Conclusion
References
11 CRISPR/Cas techniques: a new method for RNA interference in cereals
11.1 Introduction
11.2 Overview of CRISPR/Cas system
11.3 CRISPR system for genome editing in cereals
11.3.1 CRISPR/Cas system for rice improvement
11.3.2 CRISPR/Cas system for wheat improvement
11.3.3 CRISPR/Cas system for maize improvement
11.3.4 CRISPR/Cas system for sorghum improvement
11.4 CRISPR/Cas system a better choice for genome editing
11.5 Recent developments in CRISPR technology
11.6 Conclusion and future prospectus
References
12 Genetic transformation methods and advancement of CRISPR/Cas9 technology in wheat
12.1 Introduction
12.2 Objective
12.3 Background.
12.3.1 Structure and mechanism of Cas9
12.3.2 Types of CRISPR/Cas and opportunity headed for genome editing
12.4 Steps involved in CRISPR/Cas9 mediated genome editing
12.5 Different technologies evolved from CRISPR
12.5.1 Gene and epigenome editing in wheat
12.5.2 Transcriptional activation and suppression using dCas9
12.5.3 Site-directed foreign DNA insertion in the wheat genome
12.5.4 Multiplexed engineering in wheat
12.5.4.1 Multiple gRNAs with their respective promoters
12.5.4.2 Multiple gRNAs using tRNA processing enzymes
12.5.4.3 Multiple gRNAs using Csy4
12.5.5 Viral replicon based editing in wheat
12.6 The delivery methods of CRISPR/Cas9 construct in wheat
12.6.1 Biolistic mediated delivery of CRISPR/Cas9 in the wheat
12.6.2 Agrobacterium-mediated transformation in wheat
12.6.3 Floral dip/microspore-based gene editing in wheat
12.6.4 PEG-mediated delivery of CRISPR/Cas9 reagents or vector
12.7 Genome engineering for wheat improvement
12.7.1 Improvement for grain quality and stress-tolerant wheat
12.7.2 CRISPR/Cas9 mediated fungal resistant wheat
12.8 Conclusion and outlook
Acknowledgments
References
13 Application of CRISPR/Cas system for genome editing in cotton
13.1 Introduction
13.2 Genome editing technologies
13.3 CRISPR/Cas genome editing system
13.4 Application of CRISPR/Cas9 for genome editing in cotton
13.4.1 Utilization of CRISPR for biotic stresses
13.4.2 Utilization of CRISPR for abiotic stresses
13.4.3 Utilization of CRISPR for fiber quality
13.4.4 Utilization of CRISPR for plant architecture and flowering
13.4.5 Utilization of CRISPR for virus-induced disease resistance
13.4.6 Utilization of CRISPR for epigenetic modifications
13.4.7 Utilization of CRISPR for multiplexed gene stacking.