rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Advanced methods in molecular biology and biotechnology
    sub title: a practical lab manual
  name:
    name part: Masoodi, Khalid Z.
    role:
      role term: creator
  name:
    name part: Lone, Sameena Maqbool.
  name:
    name part: Rasool, Ravidha Saba.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: Electronic books.
  genre: Laboratory Manual
  genre: Laboratory manuals
  genre: Laboratory manuals.
  genre: Manuels de laboratoire.
  origin info:
    place:
      place term: enk
    place:
      place term: London
    publisher: Academic Press
    date issued: [2021]
    date issued: 2021
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource
  abstract: Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.
  table of contents: Intro -- Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual -- Copyright -- Dedication -- Contents -- Preface -- Foreword -- Acknowledgment -- Introduction -- About the author -- Disclaimer -- Chapter 1 Introduction to molecular biology techniques -- Safety measures -- Preparation of the solutions -- Working with the nucleic acids -- Storage -- Purification -- Quantitation -- Concentration -- Restriction enzymes -- Sterile techniques -- References -- Chapter 2 Agarose gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table
  table of contents: Materials and equipments -- Materials and equipments required -- Reagents required -- Step-by-step method details -- Timing: 1-1.5 h (depending on the size of the sample) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 3 SDS-polyacrylamide gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details
  table of contents: Timing: 3-4 h (depends on the plate size) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 4 Immunoelectrophoresis -- Definition -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards
  table of contents: Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- References -- Chapter 7 Genomic DNA extraction from the plant leaves using the CTAB method -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution.
  note: Khalid Z. Masoodi, Sameena Maqbool Lone and Rovidha Saba Rasool.
  subject:
    topic: Molecular biology
    genre: Laboratory manuals
  subject:
    topic: Biotechnology
    genre: Laboratory manuals
  subject:
    topic: Biologie moléculaire
    genre: Manuels de laboratoire
  subject:
    topic: Biotechnologie
    genre: Manuels de laboratoire
  subject:
    topic: Biotechnology
  subject:
    topic: Molecular biology
  classification: QH506
  classification: 572.80721
  related item:
    identifier: (OCoLC)1182571993
  identifier: 9780128244500
  identifier: 012824450X
  identifier: 9780128244494
  identifier: 0128244496
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: YDX
    record creation date: 201031
    record change date: 20260327165422.5
    record identifier: in00005767243
    language of cataloging:
      language term: eng