http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Advanced methods in molecular biology and biotechnology
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.
Intro -- Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual -- Copyright -- Dedication -- Contents -- Preface -- Foreword -- Acknowledgment -- Introduction -- About the author -- Disclaimer -- Chapter 1 Introduction to molecular biology techniques -- Safety measures -- Preparation of the solutions -- Working with the nucleic acids -- Storage -- Purification -- Quantitation -- Concentration -- Restriction enzymes -- Sterile techniques -- References -- Chapter 2 Agarose gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table
Materials and equipments -- Materials and equipments required -- Reagents required -- Step-by-step method details -- Timing: 1-1.5 h (depending on the size of the sample) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 3 SDS-polyacrylamide gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details
Timing: 3-4 h (depends on the plate size) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 4 Immunoelectrophoresis -- Definition -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards
Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- References -- Chapter 7 Genomic DNA extraction from the plant leaves using the CTAB method -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution.
Khalid Z. Masoodi, Sameena Maqbool Lone and Rovidha Saba Rasool.