rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Epigenetics methods
  name:
    name part: Tollefsbol, Trygve O.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  genre: Internet Resources.
  origin info:
    place:
      place term: enk
    place:
      place term: London
    publisher: Academic Press
    date issued: 2020
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource
  abstract: In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ. Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth. In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field. Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application. Integrates technology and translation with fundamental chapters on epigenetics met…
  table of contents: Preface -- Part One: Overview -- Chapter One: Epigenetics methods in biological research -- Part Two: DNA methylation methods: Gene- or locus-specific methylation analyses -- Chapter Two: Principles of bi-sulfite conversion of DNA and methylation-specific PCR (MSP) in biological research -- Chapter Three: Analysis of DNA methylation using pyrosequencing -- Chapter Four: Methylation-specific droplet digital PCR (MS-ddPCR) for detection and absolute quantification of rare methylated alleles -- Part Three: DNA methylation methods: Global DNA methylation and methylomic analyses -- Chapter Five: ELISA analysis of global methylation levels -- Chapter Six: Bisulfite PCR of repetitive genomic sequences -- Chapter Seven: The Illumina Infinium methylation assay for genome-wide methylation analyses -- Chapter Eight: Analysis of genome-wide methylation using reduced representation bisulfite sequencing (RRBS) technology -- Chapter Nine: Methylated DNA immunoprecipitation sequencing (MeDIP-seq): Principles and applications -- Chapter Ten: Multiplex analyses using methylation sensitive restriction enzyme qPCR -- Chapter Eleven: Advances in whole genome methylomic sequencing -- Part Four: DNA methylation methods: Additional technologies -- Chapter Twelve: Analyzing DNA methyltransferase enzymatic activity: Basic methods and applications -- Chapter Thirteen: Technologies and applications for the assessment of 5-hydroxymethylcytosine -- Part Five: Histone modification methods -- Chapter Fourteen: The chromatin immunoprecipitation (ChIP) assay and ChIP-qPCR -- Chapter Fifteen: ChIP in series: Sequential ChIP -- Chapter Sixteen: ChIP-exo: A method to study chromatin structure and organization at near-nucleotide resolution -- Chapter Seventeen: Monitoring genome-wide chromatin accessibility by formaldehyde-assisted isolation of regulatory elements sequencing (FAIRE-seq) -- Chapter Eighteen: ATAC-seq method applied to embryonic germ cells and neural stem cells from mouse: Practical tips …
  note: edited by Trygve Tollefsbol.
  note: Includes bibliographical references and index.
  subject:
    topic: Epigenetics
  subject:
    topic: Stem cells
  subject:
    topic: Stem Cells
  subject:
    topic: Epigenomics
  subject:
    topic: Épigénétique
  subject:
    topic: Cellules souches
  subject:
    topic: Epigenetics
  classification: QH450
  classification: 572.8/65
  related item:
    identifier: (OCoLC)1130261918
  related item:
    title info:
      title: Translational epigenetics series ; v. 18
  identifier: 9780128194157
  identifier: 0128194154
  identifier: 9780128194140
  identifier: 0128194146
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: YDX
    record creation date: 200714
    record change date: 20260327165034.2
    record identifier: in00005766990
    language of cataloging:
      language term: eng