http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ. Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth. In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field. Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application. Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods. Written at verbal and technical levels that can be understood by scientists and students alike Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics.
Preface -- Part One: Overview -- Chapter One: Epigenetics methods in biological research -- Part Two: DNA methylation methods: Gene- or locus-specific methylation analyses -- Chapter Two: Principles of bi-sulfite conversion of DNA and methylation-specific PCR (MSP) in biological research -- Chapter Three: Analysis of DNA methylation using pyrosequencing -- Chapter Four: Methylation-specific droplet digital PCR (MS-ddPCR) for detection and absolute quantification of rare methylated alleles -- Part Three: DNA methylation methods: Global DNA methylation and methylomic analyses -- Chapter Five: ELISA analysis of global methylation levels -- Chapter Six: Bisulfite PCR of repetitive genomic sequences -- Chapter Seven: The Illumina Infinium methylation assay for genome-wide methylation analyses -- Chapter Eight: Analysis of genome-wide methylation using reduced representation bisulfite sequencing (RRBS) technology -- Chapter Nine: Methylated DNA immunoprecipitation sequencing (MeDIP-seq): Principles and applications -- Chapter Ten: Multiplex analyses using methylation sensitive restriction enzyme qPCR -- Chapter Eleven: Advances in whole genome methylomic sequencing -- Part Four: DNA methylation methods: Additional technologies -- Chapter Twelve: Analyzing DNA methyltransferase enzymatic activity: Basic methods and applications -- Chapter Thirteen: Technologies and applications for the assessment of 5-hydroxymethylcytosine -- Part Five: Histone modification methods -- Chapter Fourteen: The chromatin immunoprecipitation (ChIP) assay and ChIP-qPCR -- Chapter Fifteen: ChIP in series: Sequential ChIP -- Chapter Sixteen: ChIP-exo: A method to study chromatin structure and organization at near-nucleotide resolution -- Chapter Seventeen: Monitoring genome-wide chromatin accessibility by formaldehyde-assisted isolation of regulatory elements sequencing (FAIRE-seq) -- Chapter Eighteen: ATAC-seq method applied to embryonic germ cells and neural stem cells from mouse: Practical tips and modifications -- Chapter Nineteen: Combinatorial ChIP and bisulfite sequencing (BisChIP-seq) -- Part Six: Epigenetic methods for evaluating chromatin higher order -- Chapter Twenty: 3D DNA FISH for analyses of chromatin-nuclear architecture -- Chapter Twenty-One: Capture Hi-C: Characterization of chromatin contacts -- Part Seven: Enzyme analyses of histone methylation -- Chapter Twenty-Two: Assessment of the epigenetic nature of chromosomal proteins during DNA replication and mitosis -- Part Eight: Assessment of non-coding RNA as an epigenetic modulator -- Chapter Twenty-Three: Detection and quantification of microRNAs (miRNAs) and high-throughput miRNA profiling -- Chapter Twenty-Four: RIP-seq in epigenetic analyses -- Chapter : Chromatin isolation by RNA purification (ChIRP) and its applications -- Part Nine: Additional epigenetics methods -- Chapter Twenty-Six: Single-cell epigenomics: Methods and translation -- Chapter Twenty-Seven: CRISPR/Cas9 technologies in epigenetics research -- Chapter Twenty-Eight: Recent advances in computational-based approaches in epigenetics studies -- Chapter Twenty-Nine : Epigenome-wide association study (EWAS): Methods and applications -- Chapter Thirty: Methods in epigenetics-based systems biology and their applications -- Chapter Thirty-One: Forensic epigenetics methods and applications -- Part Ten: Future directions in epigenetics methodology -- Chapter Thirty-Two: Advances in epigenetic techniques to study development and diseases.
Includes bibliographical references and index.
Translational epigenetics series ; v. 18