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|a 1090911132
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|a 9780128157756
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|a 0128157755
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|z 9780128180242
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|z 0128180242
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|z 9780128157749
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|z 0128157747
|q (hardcover)
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|a (OCoLC)1089831122
|z (OCoLC)1090911132
|z (OCoLC)1122755791
|z (OCoLC)1229477890
|z (OCoLC)1397906976
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| 050 |
|
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|a QH506
|b .C36 2019eb
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| 060 |
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|a 2019 D-087
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| 060 |
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|a QU 25
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|a 572.8078
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|a TXAM
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| 100 |
1 |
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|a Carson, Sue
|q (Susan),
|e author.
|1 https://id.oclc.org/worldcat/entity/E39PCjJyhrG4vWDcgFJWv99DpX
|
| 245 |
1 |
0 |
|a Molecular biology techniques :
|b a classroom laboratory manual /
|c Susan Carson, Heather B. Miller, D. Scott Witherow, Melissa C. Srougi.
|
| 250 |
|
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|a Fourth edition.
|
| 264 |
|
1 |
|a London, United Kingdom :
|b Academic Press, an imprint of Elsevier,
|c 2019.
|
| 300 |
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|a 1 online resource
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
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|2 rdamedia
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| 338 |
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|a online resource
|b cr
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| 347 |
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|a data file
|
| 500 |
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|a Includes index.
|
| 588 |
0 |
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|a Online resource; title from PDF title page (ScienceDirect, viewed March 14, 2019).
|
| 504 |
|
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|a Includes bibliographical references and index.
|
| 520 |
|
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|a Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology--or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein.
|
| 505 |
0 |
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|a Part I. Manipulation of DNA -- Getting oriented: practicing with micropipettes -- Purification and digestion of plasmid (vector) DNA -- Completion of vector preparation and polymerase chain reaction amplification of egfp -- Preparation on insert DNA (egfp) PCR product -- DNA ligation and transformation of Escherichia coli -- Part II. Screening transformants -- Screening of transformants, part I -- Screening of transformants, part II -- Analysis of DNA sequence from a positive clone -- Part III. Expression, detection, and purification of recombinant proteins from bacteria -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part I -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part II -- Extraction of recombinant protein from Escherichia coli using a glutathione affinity culture -- Analysis of purification fractions -- Part IV. Analysis of mRNA levels -- Total RNA purification -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part I -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part II -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part I -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part II -- Part V. Modulation of gene expression -- Culturing mammalian cells -- Transient transfection of mammalian cells -- RNAi-mediated knockdown of EGFP, Part I -- RNAi-mediated knockdown of EGFP, Part II -- RNAi-mediated knockdown of EGFP, Part III -- CRISPR-mediated knockout of EGFP, Part I -- CRISPR-mediated knockout of EGFP, Part II -- Advanced CRISPR, Part I -- Advanced CRISPR, Part II -- Interim laboratory session -- Advanced CRISPR, Part III -- Advanced CRISPR, Part IV -- Advanced CRISPR, Part V -- Advanced CRISPR, Part VI.
|
| 650 |
|
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|a Molecular biology
|v Laboratory manuals.
|
| 650 |
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0 |
|a Genetics
|x Technique.
|
| 650 |
1 |
2 |
|a Genetic Techniques
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| 650 |
2 |
2 |
|a Molecular Biology
|x methods
|
| 650 |
2 |
2 |
|a CRISPR-Cas Systems
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| 650 |
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6 |
|a Biologie moléculaire
|v Manuels de laboratoire.
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| 650 |
|
6 |
|a Génétique
|x Technique.
|
| 650 |
|
6 |
|a Systèmes CRISPR-Cas.
|
| 650 |
|
7 |
|a Genetics
|x Technique
|2 fast
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| 650 |
|
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|a Molecular biology
|2 fast
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| 655 |
|
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|a Electronic books.
|2 local
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| 655 |
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2 |
|a Laboratory Manual
|
| 655 |
|
7 |
|a Laboratory manuals
|2 fast
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| 655 |
|
7 |
|a Laboratory manuals.
|2 lcgft
|
| 655 |
|
7 |
|a Manuels de laboratoire.
|2 rvmgf
|
| 700 |
1 |
|
|a Miller, Heather B.,
|e author.
|
| 700 |
1 |
|
|a Srougi, Melissa C.,
|e author.
|
| 700 |
1 |
|
|a Witherow, D. Scott,
|e author.
|
| 710 |
2 |
|
|a ScienceDirect (Online service)
|
| 776 |
0 |
8 |
|i Print version:
|a Carson, Sue (Susan).
|t Molecular biology techniques.
|b Fourth edition.
|d London, United Kingdom : Academic Press, an imprint of Elsevier, 2019
|z 0128180242
|z 9780128180242
|w (OCoLC)1047781545
|
| 856 |
4 |
0 |
|u http://proxy.library.tamu.edu/login?url=https://www.sciencedirect.com/science/book/9780128180242
|z Connect to the full text of this electronic book
|t 0
|
| 955 |
|
|
|a Elsevier ScienceDirect 2026-2027
|
| 994 |
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|a 92
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| 999 |
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| 952 |
f |
f |
|a Texas A&M University
|b College Station
|c Electronic Resources
|s www_evans
|d Available Online
|t 0
|e QH506 .C36 2019eb
|h Library of Congress classification
|
| 998 |
f |
f |
|a QH506 .C36 2019eb
|t 0
|l Available Online
|