Microbiology of atypical environments /
Microbiology of Atypical Environments, Volume 45, presents a comprehensive reference text on the microbiological methods used to research the basic biology of microorganism in harsh, stressful and sometimes atypical environments (e.g.arctic ice, space stations, extraterrestrial environments, hot spr...
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
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London, United Kingdom :
Academic Press, an imprint of Elsevier,
2018.
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| Edition: | First edition. |
| Series: | Methods in microbiology ;
v. 45. |
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Front Cover; Microbiology of Atypical Environments; Copyright; Contents; Contributors; Cover Picture; Preface; Section 1: Methods for Studying Microorganisms in Space; Chapter 1: Microbiology of the Built Environment in Spacecraft Used for Human Flight; 1. Introduction; 2. Microbiological Monitoring in Human-Occupied Spacecraft; 2.1. Microorganisms in Human-Occupied Spacecraft; 3. Ground-Based Tests (Simulating Microgravity in the Laboratory); 4. Microorganisms Associated With Water Recovery; 5. Relevance of the Spacecraft Microbiome to Humans during Space Exploration
- 6. Microbial Influences on Spacecraft Integrity and Function7. Biofilms and Their Impact on Spaceflight; 8. Long-Term Studies; Conclusions; Future Directions; Acknowledgements; References; Chapter 2: The Study of Microbial Survival in Extraterrestrial Environments Using Low Earth Orbit and Ground-Based Experimen; 1. Introduction; 2. Microbiological Facilities in Space: Past and Present; 2.1. The Long Duration Exposure Facility; 2.2. EUropean REtrievable Carrier; 2.3. BIOPAN; 2.4. EXPOSE Facility; 2.5. Organism/Organic Exposure to Orbital Stresses Nanosatellite
- 3. Ground-based Simulation Facilities3.1. Simulated Mars Conditions; 3.2. Simulated Space Conditions; 3.3. Simulating Lithopanspermia; 3.3.1. Light gas gun; 3.3.2. Shock recovery experiments; 3.3.3. ESAs STONE experiment; 4. Postexposure Analyses; 4.1. Microscopic Analysis; 4.2. Metabolic Analysis; 4.3. Molecular Analyses; 5. Life Detection; 5.1. Spectroscopic Methods; 5.1.1. Infrared spectroscopy; 5.1.2. UV-visible spectroscopy; 5.1.3. Raman spectrometry; 5.2. Gas Chromatography-Mass Spectrometry; Conclusion; References; Further Reading
- Chapter 3: Persistence of Fungi in Atypical, Closed Environments: Cultivation to Omics1. Introduction; 2. International Space Station and Other Confined Facilities as Microbial Habitats; 2.1. Microbial Sample Collection and Initial Processing; 3. Progression From Culture- to Genome-Based Approach in Microbial Characterization of Confined Spaces; 3.1. Culture-Based Microbial Analysis; 3.2. DNA-Based Microbial Analyses; 3.2.1. Pyrosequencing- and Illumina-derived fungal diversity; 3.2.2. Metagenomics-derived fungal diversity and functional characterization; Conclusions and Perspectives
- AcknowledgementsReferences; Section 2: Molecular Methods; Chapter 4: Molecular Methods for Studying Microorganisms From Atypical Environments; 1. Introduction; 2. Current Status of Microbial Diversity at Atypical Environments; 3. Application of Molecular Tools for Studying Atypical Microbial Diversity; 3.1. Whole Community Analysis; 3.1.1. DNA-DNA hybridization (DDH); 3.1.2. Analysis of G+C content; 3.1.3. Complete microbial genome sequencing; 3.1.4. Use of metagenomics; 3.2. Partial Community Analysis; 3.2.1. Clone library method; 3.2.2. Genetic fingerprinting techniques