http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Principles and applications of clinical mass spectrometry
small molecules, peptides, and pathogens
Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens, derived from the Sixth Edition of the bestselling Tietz Textbook of Clinical Chemistry and Molecular Diagnostics is a concise resource for the quick implementation of mass spectrometry methods in clinical laboratory work. Focusing on the practical use of these techniques, the book covers principles of chromatographic separations and types of mass spectrometers, along with sample preparations for analysis. Additional sections provide guidance on the use of the technology in the main applications in the clinical lab, including determination of small molecules and peptides, and in pathogen identification. Provides concrete examples on the main applications of this technologyAuthored, and edited, by the top experts in the United StatesDescribes the current capabilities of the LC- and MS-based analytical methods, along with the recipes for successful analytical work in the field
Intro; Title page; Table of Contents; Copyright; Contributors; Preface; 1. Chromatography; Basic Principles of Chromatography; Gas Chromatography; Liquid Chromatography; Other Chromatographic Methods; 2. Mass Spectrometry; Basic Concepts and Definitions; Instrumentation; Clinical Applications; Optimization of Instrument Conditions; 3. Sample Preparation for Mass Spectrometry Applications; Background; General Techniques; Separation Techniques; Precipitation Techniques; Enrichment Techniques; Extraction Techniques; Chromatographic Techniques; Evolving Techniques
4. Mass Spectrometry Applications in Infectious Disease and Pathogens IdentificationMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry; Analysis of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Data; Electrospray Ionization Mass Spectrometry; Conclusions; 5. Development and Validation of Small Molecule Analytes by Liquid Chromatography-Tandem Mass Spectrometry; Practical Considerations for Liquid Chromatography-Tandem Mass Spectrometry Assays; Sample Preparation; 6. Proteomics; Historical Perspective; Biomarker Pipeline
Bottom-Up Targeted ProteomicsTop-Down Proteomics; Preanalytical and Other Technical Considerations; Conclusions; Index
edited by Nader Rifai, PhD, Andrea Rita Horvath, MD, PhD, Carl T. Wittwer MD, PhD ; associate editor Andrew N. Hoofnagle, MD, PhD.
Includes bibliographical references and index.