Liquid chromatography. Volume 2, Applications /

Liquid Chromatography: Applications, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety...

Full description

Bibliographic Details
Corporate Author:	ScienceDirect (Online service)
Other Authors:	Fanali, Salvatore (Editor), Haddad, Paul R. (Editor), Poole, Colin (Editor), Riekkola, Marja-Liisa (Editor)
Format:	eBook
Language:	English
Published:	Amsterdam, Netherlands : Elsevier, [2017]
Edition:	Second edition.
Subjects:	Liquid chromatography. Chromatographie en phase liquide. liquid chromatography. SCIENCE / Chemistry / Analytic Liquid chromatography Electronic books.
Online Access:	Connect to the full text of this electronic book

Table of Contents:

Front Cover
Liquid Chromatography: Applications
Copyright
Contents
Contributors
Chapter 1: Sample preparation for liquid chromatography
1.1 Introduction
1.2 Overview
1.2.1 Objectives of Sample Preparation
1.2.2 Classification of Sample Preparation
1.2.3 Automation of Sample Preparation
1.2.3.1 Robotic sample preparation systems
1.2.3.2 Column switching sample preparation
1.3 Sample Extraction Techniques
1.3.1 Liquid-Phase Microextraction
1.3.1.1 DLLME
1.3.1.2 SDME
1.3.1.3 HF-LPME
1.3.2 Solid-Phase Extraction
1.3.2.1 SPE devices and processing steps
1.3.2.2 On-line column switching SPE
1.3.2.3 Sorbent selection and coating materials for SPE
1.3.3 Solid-Phase Microextraction
1.3.4 Fiber SPME
1.3.4.1 Fiber SPME processing steps for HPLC
1.3.4.2 Optimization of fiber SPME methods
1.3.4.3 Fiber coating materials
1.3.5 In-tube SPME
1.3.5.1 In-tube SPME processing systems
1.3.5.2 Optimization of in-tube SPME methods
1.3.5.3 Capillary coating materials
1.3.6 Other Sorbent Microextraction Techniques for HPLC
1.3.6.1 Static in-vessel microextraction
1.3.6.2 Dynamic in-flow microextraction
1.4 Conclusions
References
Chapter 2: Derivatization in liquid chromatography
2.1 Introduction
2.2 Reagent Selection
2.2.1 Reagents for UV-Visible Detection
2.2.2 Reagents for Fluorescence and Chemiluminescence Detection
2.2.3 Reagents for Electrochemical Detection
2.2.4 Reagents for Mass-Spectrometric Detection
2.2.4.1 Stable isotope-coded derivatizing reagents
2.2.5 Reagents for the Formation of Diastereomers
2.2.6 Multifunctional Reagents for the Formation of Cyclic Derivatives
2.2.7 Solid-Phase Analytical Derivatization
2.3 Postcolumn Reaction Detectors
2.3.1 Photoreactors
2.4 Conclusions
References.
Chapter 3: Liquid chromatographic separation of enantiomers
3.1 Introduction
3.2 Short History of Chiral LC Separations
3.3 Materials for LC Separation of Enantiomers
3.4 Modes of LC Separation of Enantiomers
3.4.1 Analytical Scale Separation of Enantiomers
3.4.2 Preparative Scale Separation of Enantiomers in LC
3.5 Separation of Enantiomers in Supercritical Fluid Chromatography (SFC)
3.6 Current Trends
3.7 Future Needs
References
Chapter 4: Amino acid and bioamine separations
4.1 Introduction
4.2 Direct Separation of Amino Acids
4.2.1 Postcolumn Colorimetric and Fluorescence Derivatization of Amino Acids
4.2.2 ESI-MS/MS Determination of Underivatized Amino Acids
4.3 Indirect Separation of Amino Acids
4.3.1 Derivatization With UV-VIS Reagents
4.3.2 Derivatization With Fluorescent Reagents
4.3.3 Derivatization for Mass Spectrometric Detection
4.4 Enantioselective Liquid Chromatographic Analysis of Amino Acids
4.4.1 Chiral Derivatization Reagents for Amino Acid Enantiomers
4.4.2 Chiral Stationary Phases for Amino Acid Enantiomers
4.4.3 Two-Dimensional Liquid Chromatographic Analysis of Amino Acid Enantiomers
4.5 Direct Separation of Biogenic Amines
4.6 Indirect Separation of Biogenic Amines
4.7 Conclusions
References
Chapter 5: Protein and peptide separations
5.1 Introduction
5.2 Methods of Protein Liquid Chromatography
5.2.1 Size-Exclusion Chromatography
5.2.2 Ion-Exchange Chromatography
5.2.3 Methods Based on the Hydrophobic Interaction
Hydrophobic-interaction chromatography
Reversed-phase chromatography
5.2.4 Affinity Chromatography
Pseudoaffinity chromatography
Hydrophobic charge-induction chromatography
Immobilized metal-affinity chromatography
5.2.5 Chromatography on Hydroxyapatite
5.2.6 Chromatography on Monolithic Supports.
5.2.7 Displacement Chromatography
5.3 Conclusions
Acknowledgments
Addendum 1: Protein and Peptide Chromatography-References Update
Ion-exchange chromatography
Hydrophobic-interaction chromatography:
Mixed-mode and hydrophobic charge-induction chromatography:
Reversed-phase chromatography:
Size-exclusion chromatography
Displacement chromatography:
Preparative and process chromatography:
Monoliths, membranes and other special supports:
Optimization and protein and peptide characterization:
LC applications in proteomics and peptidomics:
Affinity chromatography
Protein and peptide chromatography, reviews and overviews
Addendum 2: Sample Displacement Chromatography
Introduction
Development and Use of Sample Displacement Chromatography
Conclusions
References
References
Further Reading
Chapter 6: Liquid chromatographic separation of oligonucleotides
6.1 Introduction
6.2 Oligonucleotide and siRNA Structure and Preparation
6.3 Chromatographic Separation of Oligonucleotides
6.3.1 Separation of Oligonucleotides With Ion-Exchange Liquid Chromatography
6.3.2 Separation of Oligonucleotides With IP-RPLC
6.3.2.1 Separation of oligonucleotides with IP-RPLC using core-shell particle columns
6.3.3 Separation of Oligonucleotides With Mixed-Mode Chromatography
6.4 Summary
References
Chapter 7: Separation of glycans and monosaccharides
7.1 Introduction
7.2 Types of Glycans
7.3 Analysis and Characterization of Glycans
7.3.1 Glycan Release
7.3.2 Fluorescent Labeling of Glycans
7.3.3 Hydrophilic Interaction Liquid Chromatography
7.3.4 Weak Anion-Exchange Liquid Chromatography
7.3.5 Exoglycosidase Sequencing
7.3.6 Reversed-Phase Liquid Chromatography
7.3.7 Porous Graphitic Carbon
7.4 Monosaccharide Composition Analysis.
7.4.1 Hydrolysis of Monosaccharides
7.4.2 Labeling and Analysis of Monosaccharides
7.5 Conclusion
References
Chapter 8: Separation of lipids
8.1 Introduction and Contents
8.2 Definitions and Classification
8.3 Structures and Occurrence
8.3.1 Fatty Acids
8.3.2 Glycerolipids
8.3.3 Glycerophospholipids
8.3.4 Sphingolipids
8.3.5 Sterol Lipids
8.3.6 Prenol Lipids
8.3.7 Saccharolipids
8.3.8 Polyketides
8.4 Sample Handling and Extraction
8.4.1 Sampling and Sample Preparation
8.4.2 Soxhlet Extraction
8.4.3 Method of Folch, Lees, and Stanley
8.4.4 Method of Bligh and Dyer
8.4.5 Accelerated Solvent Extraction
8.4.6 Supercritical Fluid Extraction
8.4.7 Microwave-Assisted Extraction
8.4.8 Other Extraction Methods
8.5 Lipid Analysis by LC
8.5.1 Thin-Layer Chromatography
8.5.1.1 High-Performance and Two-Dimensional TLC
8.5.1.2 Detection and Quantification in TLC
8.5.2 High-Performance Liquid Chromatography
8.5.2.1 Normal-Phase Liquid Chromatography
8.5.2.2 Silver-Ion Liquid Chromatography
8.5.2.3 Non-aqueous Reversed-Phase Liquid Chromatography
8.5.2.4 Other HPLC Techniques
8.5.3 HPLC-MS Techniques
8.5.3.1 Lipidomics and Data Processing
8.5.4 Multidimensional Liquid Chromatography (MDLC, 2DLC)
8.6 Conclusions and Future Perspectives
References
Chapter 9: Metabolic phenotyping (metabonomics/metabolomics) by liquid chromatography-mass spectrometry
9.1 Introduction
9.2 LC-MS-based approaches to metabolic phenotyping
9.2.1 Reversed-Phase HPLC and U(H)PLC/MS for Metabolic Phenotyping
9.2.2 Polar Metabolite Analysis via HILIC, Aqueous Normal Phase (ANP), and Ion Chromatography(IC)/Ion Exchange (IE) LC-MS ...
9.2.3 Multicolumn and Multidimensional LC Separations
9.2.4 Miniaturization
9.3 Supercritical fluid chromatography (SFC).
9.4 Ion Mobility Spectrometry
9.5 Conclusions
References
Chapter 10: Foodomics: LC and LC-MS-based omics strategies in food science and nutrition
10.1 Introduction
10.2 Fundamentals of omics approaches based on LC
10.2.1 Proteomics
10.2.2 Peptidomics
10.2.3 Metabolomics
10.2.4 Lipidomics
10.2.5 Glycomics
10.3 LC-based foodomics applications
10.3.1 Food Bioactivity
10.3.2 Food Safety
10.3.2.1 Chemical contaminants
10.3.2.2 Pathogens and toxins
10.3.2.3 Food allergens
10.3.3 Food Quality, Authenticity, and Traceability
Acknowledgments
References
Chapter 11: Forensic toxicology
11.1 General drug screening
11.1.1 Extraction Techniques
11.1.2 Screening Using Diode Array Detection
11.2 Liquid chromatography-mass spectrometry: background and considerations
11.2.1 Atmospheric Pressure Ionization Sources: APCI, ESI
11.2.2 ESI and Mobile Phase pH
11.2.3 Atmospheric-Pressure Chemical Ionization
11.2.4 General Practical Considerations for LC-MS
11.3 Forensic toxicology LC-MS applications
11.3.1 Overview
11.3.2 Single Quadrupole Instruments
11.3.3 Time-of-Flight Instruments
11.3.4 Orbitrap Analysers
11.3.5 Low Resolution Ion Traps
11.3.6 Data Dependent Acquisition and Data Independent Acquisition for Broad Screening
11.4 LCMS identification criteria in forensic toxicology
11.4.1 The Continuing Relevance of Chromatography
11.4.2 MS Identification Criteria
11.5 Validation and matrix effects
11.5.1 Validation Requirements
11.5.2 Matrix Effects
11.6 Testing for driving under the influence of drugs using oral fluids
11.6.1 Analytical Methodology
11.6.2 Sample Preparation
11.6.3 LC-Tandem MS
11.6.4 Liquid Chromatography Analysis of Oral Fluid-Conclusions and Future Directions.