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Essentials of noncoding RNA in neuroscience
ontogenetics, plasticity of the vertebrate brain
Essentials of Noncoding RNA in Neuroscience: Ontogenetics, Plasticity of the Vertebrate Brain focuses on the role of miRNAs in neurogenesis, gliogenesis, neuronal network formation, and the cell biology of forebrain development. The important role miRNAs play in neuronal maturation, neocortex function, and in some neurodevelopmental disorders is discussed, as are the computational challenges and methods used in the identification of miRNA targets. This book is a valuable reference for neuroscientists who wish to better understand the role of miRNAs in complex processes. It is of strong interest to those working to develop enabling technologies to detect and monitor miRNA expression and function, and to evaluate its roles in neural progenitor proliferation/differentiation, neuronal plasticity, and learning and memory.
Front Cover; Essentials of Noncoding RNA in Neuroscience; Copyright Page; Contents; List of Contributors; Preface; Acknowledgments; 1 Making and Maintaining MicroRNAs in Animals; Introduction; Genomic Organization; MicroRNA Transcription; Primary MicroRNA Processing; Export From the Nucleus; Pre-MicroRNA Processing; Mature MicroRNA Complex Formation; MicroRNA Stability; Concluding Remarks; Acknowledgements; References; 2 Essentials of miRNA-dependent Control of mRNA Translation and decay, miRNA Targeting Principles, and Methods for Target I ...
MiRNAs are Posttranscriptional Regulators of Gene ExpressionMiRNA Binding Guides AGO Function to Target RNAs; The Role of the miRNA Seed and the Kinetics of Binding; Alternative 3'UTRs Usage and the Molecular Mechanisms of miRNA-Mediated Translational Repression; MiRNAs Buffer Expression Noise to Confer Robustness to Biological Pathways and Act in a Compensatory Manner; Identification of miRNA Targets via Permutation Experiments and Bioinformatics Prediction; Identification of miRNA Sites by Recovering AGO-Bound RNA; Crosslinking and Immunoprecipitation to Uncover Target Sites of RBPs
Argonaute-Clip Identifies Sites of miRNA Binding but the Targeting Remains UncertainDirect Identification of miRNA:Target Site Interactions by Ligation; Ligases Connect the miRNAs Directly to Their Bound Target Sites Resulting in Chimeric Molecules; Chimeras Recover Endogenous miRNA Interactions, the Majority Possesses Seed Complementarity; Research Opportunities Opened Up by Chimeras: Conservation Analysis of Imperfect Seed Sites and 32 nd Pairing, Classificati ... ; Functionality of Chimera-Identified Interactions; Perspectives; References
3 Computational Challenges and -omics Approaches for the Identification of microRNAs and TargetsIntroduction; MiRNA Annotation and Regulation; MiRNA-Transcription Start Sites (TSSs); Databases of miRNA regulation; MiRNA-Gene Interactions; Computational Approaches for the Identification of miRNA-mRNA Predicted Interactions; Databases Cataloging Experimentally Supported miRNA-mRNA-lncRNA Interactions; Investigating miRNA-lncRNA interactions; Functional Analysis Tools and Functional Annotation Databases; MiRNA Functional Analysis and Functional Annotation
Identification of miRNA-Controlled Pathways and miRNA FunctionsIdentification of Functional miRNAs Using Expression Data; State-of-the-Art Computational-Experimental Approaches; Closing Remarks; References; 4 Methodological Challenges in Functional Investigation and Therapeutic Use of MicroRNAs; RNA Isolation Methods; Analysis of miRNAs Expression; Functional Investigation of miRNAs; Approaches for Loss of miRNA Function; Approaches for Gain-of miRNA Function; Delivery of miRNA Antagonists or Mimics; Viral Vectors; Nonviral carriers; MiRNA:Target Validation; Indirect Validation
Includes bibliographical references and index.
London, United Kingdom : Academic Press, n imprint of Elsevier, [2017]