http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Enzymes as Sensors, Volume 589 in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics that presents a micro-level delivery of specific content related to the study of enzymes in sensors. New to this edition are highly specialized chapters on integrated strategies for gaining a systems level view of dynamic signaling networks, sensitive protein detection and quantification in paper-based microfluidics for point-of-care, and microneedle enzyme sensor arrays for continuous in vivo monitoring.
Recent advances in development of genetically encoded fluorescent sensors -- Engineering rugged field assays to detect hazardous chemicals using spore-based bacterial biosensors -- Engineering BRET-sensor proteins -- Enzymes as sensors -- Integrated strategies to gain a systems-level view of dynamic signaling networks -- Probing Cdc42 polarization dynamics in budding yeast using a biosensor -- Novel fluorescence-based biosensors incorporating unnatural amino acids -- Folding- and dynamics-based electrochemical DNA sensors -- Construction of protein-based biosensors using ligand-directed chemistry for detecting analyte binding -- Measuring and imaging metal ions with fluorescence-based biosensors: speciation, selectivity, kinetics, and other issues -- Bioapplications of electrochemical sensors and biosensors -- A highly sensitive biosensor for ATP using a chimeric firefly luciferase -- Highly modular bioluminescent sensors for small molecules and proteins -- Sensitive protein detection and quantification in paper-based microfluidics for the point of care -- Microneedle enzyme sensor arrays for continuous in vivo monitoring -- Visualization of the genomic loci that are bound by specific multiprotein complexes by biomolecular fluorescence complementation analysis on Drosophila polytene chromosomes -- Author index -- Subject index.
edited by Richard B. Thompson, Carol A. Fierke.
Includes bibliographical references and index.