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Microbial cellulase system properties and applications
New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Cellulase System Properties and Applications covers the biochemistry of cellulase system, its mechanisms of action, and its industrial applications. Research has shed new light on the mechanisms of microbial cellulase production and has led to the development of technologies for production and applications of cellulose degrading enzymes. The biological aspects of processing of cellulosic biomass have become the crux of future research involving cellulases and cellulolytic microorganisms, as they are being commercially produced by several industries globally and are widely being used in food, animal feed, fermentation, agriculture, pulp and paper, and textile applications. The book discusses modern biotechnology tools, especially in the area of microbial genetics, novel enzymes, and new enzyme and the applications in various industries. As a professional reference, this new book is useful to all researchers working with microbial cellulase system, both academic institutions and industry-based research bodies, as well as to teachers, graduate, and postgraduate students with information on continuous developments in microbial cellulase system. The book provides an indispensable reference source for chemists, biochemical engineers/bioengineers, biochemists, biotechnologists and researchers who want to know about the unique properties of this microbe and explore its future applications. Compiles the latest developments made and currently undergoing in the area of microbial cellulase system Chapters are contributed from top researchers on this area around the globe Includes information related to almost all areas of microbial cellulase system Extensive cover of current industrial applications and discusses potential future applications.
Front Cover; New and Future Developments in Microbial Biotechnology and Bioengineering; Copyright Page; Contents; List of Contributors; I. Penicillium: Biology to Biotechnology; 1 Biodiversity of the Genus Penicillium in Different Habitats; 1.1 Introduction; 1.2 Isolation and Characterization of Penicillium; 1.3 Molecular Diversity and Phylogenetic Analysis; 1.4 Distribution and Abundance of Penicillium; 1.4.1 Low/High-Temperature Habitat; 1.4.2 Saline Habitat; 1.4.3 Acidic/Alkaline Habitat; 1.4.4 Penicillium Associated With Plants and Fruits; 1.5 Conclusion and Future Prospects; References.
2 Understanding the Diversity of Penicillium Using Next-Generation Sequencing 2.1 Introduction; 2.2 Isolation of Penicillium species; 2.2.1 Isolation From Air; 2.2.2 Isolation From Soil Samples; 2.2.3 Isolation From Food Samples; 2.3 Detection of Penicillium species; 2.3.1 Conventional Techniques; 2.3.2 Molecular Techniques; 2.3.2.1 Preparation of Fungal Culture for Toxin Detection; 2.3.2.2 DNA Extraction From Fungal Cultures for Molecular Identification; 2.3.2.3 Determining DNA Concentration and Purity; 2.3.2.4 Polymerase Chain Reaction; 2.3.2.5 PCR Detection of Penicillium Species.
2.4.3.1.3 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Fingerprinting (MALDI-TOF MS)2.4.3.2 Cloning and Sequencing of PCR Products; 2.4.3.2.1 Preparation and Analysis of DNA Heteroduplexes; 2.4.3.2.2 Identification of Polymorphic Genetic Loci; 2.4.3.3 Next-Generation Sequencing; 2.4.3.3.1 Roche 454 FLX Pyrosequencer; 2.4.3.3.2 Illumina Genome Analyzer; 2.4.3.3.3 Applied Biosystem SOLiD Sequencer; 2.4.3.3.4 Heliscope Single-Molecule Sequencer; 2.4.3.3.5 Ion Personal Genome Machine (PGM); 2.4.3.3.6 RNA-Seq.
2.4.3.3.7 Pacific Biosciences SMRT Sequencing DNA Sequencer2.4.3.4 Submitting DNA Fragment for Sequencing; 2.4.3.4.1 Using Bioinformatics; 2.5 Conclusions and Future Perspectives; References; Further Reading; II. Secondary Metabolism; 3 Secondary Metabolism and Antimicrobial Metabolites of Penicillium; 3.1 Introduction; 3.2 Secondary Metabolism in Fungi; 3.2.1 Secondary Metabolism as Differentiation; 3.3 Microorganisms as Sources of Secondary Metabolites; 3.3.1 Bioactivities of Microbial Secondary Metabolites; 3.3.2 Biotechnological Importance of Fungi; 3.3.2.1 Ergot Alkaloids.
Includes bibliographical references and index.
New and future developments in microbial biotechnology and bioengineering