rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Structures of Large RNA Molecules and Their Complexes
  name:
    name part: Woodson, Sarah A.
    role:
      role term: editor.
  name:
    name part: Allain, Frédéric H. T.
    role:
      role term: editor.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: Electronic books.
  genre: Internet Resources.
  origin info:
    place:
      place term: mau
    date issued: 2015
    edition: First edition.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (xxi, 631 pages, 20 unnumbered pages of plates : illustrations (some color)).
  abstract: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs.
  table of contents: Front Cover; Structures of Large RNA Molecules and Their Complexes; Copyright; Contents; Contributors; Preface; Section I: RNA Structure and Dynamics; Chapter 1: Native Purification and Analysis of Long RNAs; 1. Introduction; 2. Native Purification of Long Noncoding RNAs; 2.1. Construct design; 2.2. DNA plasmid linearization; 2.3. In vitro transcription of RNA; 2.4. DNase digestion; 2.5. Proteinase K treatment; 2.6. EDTA chelation of divalent ions (optional); 2.7. Buffer exchange and purification; 2.8. Size-exclusion chromatography.
  table of contents: 3. Study of the RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation3.1. Preparation of samples for a study of RNA folding; 3.2. Assembly of the optical cells, sample loading, and instrument setup; 3.3. Setting up a sedimentation velocity experiment; 3.4. Data analysis; 4. Analysis of the RNA Tertiary Folding by Analytical Size-Exclusion Chromatography; 5. Determination of the Secondary Structure of LncRNAs by Chemical Probing; 5.1. Designing and coupling of primers; 5.2. Generation of sequencing ladders; 5.3. SHAPE reaction; 5.4. DMS reaction.
  table of contents: 5.5. Primer extension reaction5.6. Reactions for mobility shift correction; 5.7. Spectral calibration of the instrument; 5.8. Preparation of samples for capillary electrophoresis; 5.9. Data analysis; 5.9.1. Determination of chemical probing reactivity profiles; 5.9.2. Normalization of SHAPE and DMS reactivity profiles; 5.9.3. RNA secondary structure prediction and analysis; Acknowledgments; References; Chapter 2: Characterizing RNA Excited States Using NMR Relaxation Dispersion; 1. Introduction; 2. NMR Relaxation Dispersion; 2.1. Chemical Exchange; 2.2. RD Experiments.
  note: edited by Sarah A. Woodson, Department of Biophysics, Johns Hopkins University, USA, Frédéric H.T. Allain, Institute of Molecular Biology and Biophysics, ETH Zürich, Switzerland.
  note: Includes index.
  note: Text in English.
  subject:
    topic: RNA
  subject:
    topic: RNA
    topic: ultrastructure
  subject:
    topic: RNA
  subject:
    topic: ARN
  subject:
    topic: SCIENCE
    topic: Life Sciences
    topic: Biochemistry
  subject:
    topic: RNA
  classification: QP623 .S77 2015eb
  classification: 572.8/8
  classification: QU 58.7
  related item:
    title info:
      title: Structures of Large RNA Molecules and Their Complexes
    name:
      name part: Woodson, Sarah A.
    origin info:
      publisher: Burlington : Elsevier Science, ©2015
  related item:
    title info:
      title: Methods in enzymology ; v. 558
  identifier: 9780128019368
  identifier: 0128019360
  identifier: 9780128019344
  identifier: 0128019344
  identifier: 822652 MIL
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: N$T
    record creation date: 150609
    record change date: 20260326213530.2
    record identifier: in00005759280
    language of cataloging:
      language term: eng