rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Riboswitch Discovery, Structure and Function
  name:
    name part: Burke, Donald H.
    role:
      role term: editor.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  genre: Internet Resources.
  genre: Index not Present.
  origin info:
    place:
      place term: mau
    date issued: 2014
    copyright date: 2014
    edition: First edition.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (xxiii, 546 pages, 24 leaves of plates) : illustrations (some color)).
  abstract: This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, and ligand interactions and thermodynamics. Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in biomineralization science. Contains sections on such topics as riboswitch discovery and valid.
  table of contents: Riboswitch discovery by combining RNA-seq and genome-wide indentification of transcriptional start sites -- Discoverying human RNA aptamers by structure-based bioinformatics and genome-based in vitro selection -- Affinity purification of in vitro transcribed RNA with homogenous ends using a 3'-ARiBo tag -- Deoxyribozyme-mediated ligation for incorporating EPR spin labels and reporter groups into RNA -- A flexible, scalable method for preparation of homogenous aminoacylated tRNAs -- Synthesis of a biotinylated photocleavable nucleotide monophosphate for the preparation of natively folded RNAs -- Chemo-enzymatic synthesis of selectively 13C/15N-labeled RNA for NMR structural and dynamics studies -- SHAPE analysis of small RNAs and riboswitches -- Experimental approaches for measuring pKa's in RNA and DNA -- Crystallographic analysis of TPP riboswitch binding by small-molecule ligands discovered through fragment-based drug discovery approaches -- Methods for using new conceptual tools and parameters to assess RNA structure by small-angle X-ray scattering -- Use of 19F NMR methods to probe conformational heterogeneity and dynamics of exchange in functional RNA molecules -- Site-directed spin-labeling strategies and electron paramagetic resonance spectroscopy for large riboswitches -- Using sm-FRET and denaturants to reveal folding landscapes -- Riboswitch structure and dynamics by smFRET microscopy -- Ribosome structure and dynamics by smFRET microscopy -- Unraveling the thermodynamics and kinetics of RNA assembly: surface plasmon resonance, isothermal titration calorimetry, and circular dichroism -- ITC analysis of ligand binding to PreQ1 riboswitches -- Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance -- The AdoCbl-riboswitch interaction investitaged by in-line probing and surface plasmon resonance spectroscopoy (SPR) -- Assessing RNA interactions with proteins by DRaCALA.
  note: edited by Donald H. Burke-Aguero
  note: Text in English.
  note: Includes bibliographical references at the end of each chapters and indexes.
  subject:
    topic: Small interfering RNA
  subject:
    topic: Riboswitches
  subject:
    topic: Gene Expression Regulation
    topic: physiology
  subject:
    topic: Riboswitch
  subject:
    topic: RNA, Small Interfering
  subject:
    topic: Petits ARN interférents
  subject:
    topic: Riborégulateurs
  subject:
    topic: SCIENCE
    topic: Life Sciences
    topic: Biochemistry
  subject:
    topic: Riboswitches
  subject:
    topic: Small interfering RNA
  classification: QP623.5.S63 .R536 2014eb
  classification: 572.88
  classification: W1 ME9615K v.549 2014
  classification: QU 25
  related item:
    title info:
      title: Riboswitch discovery, structure, and function
    origin info:
      publisher: Amsterdam : Elsevier Academic Press, 2014
    identifier: (OCoLC)907514913
  related item:
    title info:
      title: Methods in enzymology ; v. 549
  identifier: 9780128013359
  identifier: 0128013354
  identifier: 9780128011225
  identifier: 012801122X
  identifier: 785210 MIL
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: E7B
    record creation date: 141211
    record change date: 20260326212730.2
    record identifier: in00005758500
    language of cataloging:
      language term: eng