http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
laboratory manual of analytical methods of protein chemistry, including polypeptides
The separation and isolation of proteins
A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides), Volume 1: The Separation and Isolation of Proteins deals with the techniques used in the separation and isolation of proteins, including fractionation and characterization by dialysis, multi-membrane electrodecantation, and zonal density gradient electrophoresis. The fractionation of proteins by adsorption and ion exchange is also described.
Front Cover; The Separation and Isolation of Proteins; Copyright Page; Table of Contents; PREFACE; CONTENTS LIST: VOLUME 2; CONTENTS LIST: VOLUME 3; Chapter 1. SEPARATION OF PROTEINS; A. EXTRACTION OF PROTEINS FROM NATURAL SOURCES; B. REMOVAL OF LIPIDS FROM LIPOPROTEINS; C. DENATURATION OF PROTEINS; D. NEUTRAL SALTS FOR THE FRACTIONATION AND PURIFICATION OF PROTEINS; E. THE FRACTIONATION AND PURIFICATION OF PROTEINS BY ORGANIC SOLVENTS; F. THE FRACTIONATION AND PURIFICATION OF PROTEINS WITH HEAVY METAL IONS; REFERENCES; Chapter 2. ISOLATION OF BIOLOGICALLY ACTIVE PROTEINS
Chapter 4. FRACTIONATION AND CHARACTERIZATION BY DIALYSISNATURE OF PROCESS; THE ANALYTICAL DIALYSIS CELL; SEPARATION BY DIALYSIS; THE EFFECT OF TEMPERATURE; SOLVENT EFFECTS; MEMBRANES; MEMBRANE SELECTIVITY; REFERENCES; Chapter 5. PARTITION; THE NATURE OF PARTITION; FUNDAMENTAL OPERATION OF THE TRAIN; METHODS OF OPERATION OF A DISTRIBUTION TRAIN; LOADING THE APPARATUS; INTRODUCTION OF THE SAMPLE; ATTAINMENT OF HIGHER PURITY BY SACRIFICING YIELD; TYPES OF COUNTERCURRENT DISTRIBUTION TRAINS AVAILABLE; CARE AND WASHING OF APPARATUS; ANALYTICAL TECHNIQUES OF SPECIAL VALUE
DETERMINATION OF MOLECULAR WEIGHT BY PARTITIONCONTINUOUS PARTITION; REFERENCES; Chapter 6. MULTI-MEMBRANE ELECTRODECANTATION; A. HISTORICAL INTRODUCTION; B. PRINCIPLE AND THEORY; C. CONSTRUCTION OF A MULTI-MEMBRANE ELECTRODECANTATION APPARATUS; D. OPERATION OF THE MULTI-MEMBRANE ELECTRODECANTATION APPARATUS*; E. DISCUSSION OF M.M.E.D. AND E-C; F. APPLICATION OF THE METHOD; REFERENCES; Chapter 7. ZONAL DENSITY GRADIENT ELECTROPHORESIS; INTRODUCTION; HISTORICAL; STABILITY CONDITIONS; CAPACITY; ELECTROCHEMICAL SEPARATION PRINCIPLES; THE INFLUENCE OF CONDUCTANCE GRADIENTS
THE INFLUENCE OF VISCOSITY GRADIENTSMETHODS FOR PREPARING DENSITY GRADIENT COLUMNS; ELECTRODE REACTION PRODUCTS AND THEIR POSSIBLE INTERFERENCE IN THE SEPARATION; ELECTROPHORESIS EQUIPMENT AND EXPERIMENTAL PROCEDURE; METHODS FOR VISUALIZATION OF ZONES IN THE LIQUID COLUMN; APPLICATIONS AND RESULTS; REFERENCES; AUTHOR INDEX; SUBJECT INDEX
Includes bibliographical references and index.
Separation and Isolation of Proteins : a Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides)