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Histochemical techniques /

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Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemi...

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Bibliographic Details
Main Author: Bancroft, John D. (Author)
Corporate Author: ScienceDirect (Online service)
Other Authors: Stevens, A. (Author of introduction, etc.), Pearse, A. G. E. (Author of introduction, etc.)
Format: eBook
Language:English
Published: London, England : Butterworths, 1975.
Edition:Second edition.
Subjects:
Histochemistry > Technique.
Histology > Technique.
Histocytochemistry > Laboratory manuals.
Histological Techniques
Histochimie > Technique.
Histologie > Technique.
SCIENCE > Life Sciences > Biology.
Histochemistry > Technique
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Front Cover; Histochemical Techniques; Copyright Page; Table of Contents; FOREWORD; PREFACE; Chapter 1. Introduction; Chapter 2. Fixation; PRESERVATION OF THE TISSUE; GENERAL CONSIDERATIONS; FIXING SOLUTIONS; FIXATIVES FOR USE IN HISTOCHEMISTRY; REFERENCES; Chapter 3. Paraffin Sections; REFERENCES; Chapter 4. Frozen Sections; USES OF FROZEN SECTIONS; USE OF FREEZING MICROTOME; USE OF THERMOMODULE ATTACHMENT; REFERENCES; Chapter 5. Cryostats; DEVELOPMENT OF CRYOSTATS; CRYOSTAT TECHNIQUE; REFERENCES; Chapter 6. Freeze Drying and Freeze Substitution; FREEZE DRYING TECHNIQUE.
  • HISTORY AND TYPES OF FREEZE DRYERSHOW A THERMOMODULE WORKS; THERMOELECTRIC FREEZE DRYING; FREEZE SUBSTITUTION; REFERENCES; Chapter 7. Carbohydrates; BLOCKING AND ENZYME METHODS; REFERENCES; Chapter 8. Proteins and Amino Acids; STAINING METHODS; FIXATIVES; REFERENCES; Chapter 9. Amyloid; REFERENCES; Chapter 10. Lipids; DEMONSTRATION AND IDENTIFICATION; IDENTIFICATION OF LIPIDS; REFERENCES; Chapter 11. Nucleic Acids; DIGESTION METHODS; REFERENCES; Chapter 12. Pigments; ARTEFACT PIGMENTS; ENDOGENOUS PIGMENTS; EXOGENOUS PIGMENTS; METHODS FOR PIGMENTS; REFERENCES; Chapter 13. Minerals.
  • HISTOGHEMICAL METHODSREFERENCES; Chapter 14. Principles of Enzyme Histochemistry; REFERENCES; Chapter 15. Phosphatases; INTRODUCTION; ALKALINE PHOSPHATASES; ACID PHOSPHATASES; SPECIFIC PHOSPHATASES; REFERENCES; Chapter 16. Esterases; HISTOCHEMIGAL METHODS FOR THE DEMONSTRATION OF ESTERASES; REFERENCES; Chapter 17. Oxidoreductases: I. Oxidases; HISTOCHEMIGAL METHODS; REFERENCES; Chapter 18. Oxidoreductases: II. Dehydrogenases; HISTOCHEMICAL METHODS; PREPARATION OF SECTIONS AND INCUBATING METHODS FOR DEHYDROGENASE TECHNIQUES; PRACTICAL DEMONSTRATION OF DEHYDROGENASES; REFERENCES.
  • Chapter 19. Miscellaneous TechniquesHISTOCHEMIGAL METHODS; REFERENCES; Chapter 20. Ultra-histochemis try; FIXATION; SECTIONS FOR ELECTRON CYTOCHEMISTRY; ENZYME DEMONSTRATION; ULTRASTRUGTURAL DEMONSTRATION OF SOME COMMON ENZYMES; REFERENCES; APPENDIX; Appendix I: LIST OF METHODS; Appendix II: PREPARATION OF DYES AND MOUNTING MEDIA; Appendix III: DIAZONIUM SALTS; Appendix IV: BUFFER TABLES AND PREPARATION OF MOLAR AND NORMAL SOLUTIONS; Appendix V: SOME SOURCES OF SUPPLY FOR THE CHEMICALS AND REAGENTS USED IN THE METHODS GIVEN IN THIS BOOK; Index.