http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
1 online resource (xv, 347 pages) : illustrations
Enzymatic Methods of Analysis attempts to cover all the aspects of modern enzymatic analysis. Enzymes possess a great potential usefulness in analytical chemistry. The specificity of enzymes can solve the primary problem of most analytical chemists-the analysis of one substance in the presence of many similar compounds that interfere in the analysis. The book begins with two introductory chapters devoted to general considerations of enzymes as reagents and methods of analysis of enzymatic reactions. The next four chapters deal with methods for the assay of specific enzymes, and substrate, act.
Front Cover; Enzymatic Methods of Analysis; Copyright Page; Table of Contents; PREFACE; Dedication; CHAPTER 1. GENERAL CONSIDERATIONS; A. PRINCIPLES OF ENZYMATIC ANALYSIS; B. PROPERTIES OF ENZYMES; C. FACTORS INFLUENCING THE ENZYME REACTION RATE; D. DETERMINATION OF CONCENTRATIONS; E. HANDLING BIOCHEMICAL REAGENTS; F. SOURCES OF REAGENTS; G. ENZYME ACTIVITY; REFERENCES; CHAPTER 2. METHODS OF ASSAY; A. CHEMICAL METHODS; B. INSTRUMENTAL METHODS; REFERENCES; CHAPTER 3. DETERMINATION OF ENZYMES; A. GENERAL; B. HYDROLYTIC ENZYMES; C. OXIDATIVE ENZYMES; D. DEHYDROGENASE ENZYMES.
E. kinases and transaminasesf. other enzymes; chapter 4. determination of substrates; a. general; b. carbohydrates; c. amines; d. amino acids; e. organic acids; references; chapter 5. determination of activators and coenzymes; a. general; b. determination of inorganic substances; c. determination of coenzymes; references; chapter 6. determination of inhibitors; a. general; b. determination of inorganic substances; c. determination of pesticides; d. determination of other organics; references; chapter 7. the immobilized enzyme; a. general; b. immobilization in starch gel.
C. immobilization in polyacrylamide gelsd. other methods for immobilizing enzymes; e. commercial availability of immobilized enzymes; f. future applications; references; chapter 8. use of automation in enzymic analysis; a. general; b. initial slope method; c fixed concentration (variable time) methods; d. fixed time method; references; appendix; author index; subject index; other titles in the series in analytical chemistry.
International series of monographs in analytical chemistry ; v. 34