rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Laboratory methods in enzymology
    part number: Volume 541, Part C
    part name: Protein
  title info:
    title: Protein
  name:
    name part: Lorsch, Jon
    role:
      role term: editor.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  origin info:
    place:
      place term: ne
    date issued: 2014
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (xvii, 272 pages) : illustrations (black and white)
  abstract: In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.
  table of contents: Front Cover; Laboratory Methods in Enzymology: Protein Part C; Copyright; Contents; Contributors; Miscellaneous; Preface; Section I: Protein Protocols/Protein Precipitation; Chapter One: TCA Precipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 4.3. Caution; 5. Step 1A Trichloroacetic Acid Precipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 6. Step 1B Deoxycholate-Trichloroacetic Acid Precipitation; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; References.
  table of contents: Referenced Protocols in Methods NavigatorSection II: Protein Protocols/Protein Pull-Down Methods; Chapter Two: Coimmunoprecipitation of Proteins from Yeast; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Whole Cell Lysates; 5.1. Overview; 5.2. Duration; 6. Step 2 Normalization of Cell Lysates; 6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Coimmunoprecipitation; 7.1. Overview; 7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Tip; 7.6. Tip; 7.7. Tip; 7.8. Tip; 7.9. Note.
  table of contents: 8. Step 4 Wash and Elute the Immunoprecipitates8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 9. Step 5 Analysis of Immunoprecipitations; 9.1. Overview; 9.2. Duration; References; Referenced Literature; Related Literature; Referenced Protocols in Methods Navigator; Chapter Three: Coupling Antibody to Cyanogen Bromide-Activated Sepharose; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Antibody and Resin; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 5.6. Tip.
  table of contents: 6. Step 2 Coupling the Antibody to the Resin6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Quench the Reaction; 7.1. Overview; 7.2. Duration; 7.3. Tip; 8. Step 4 Wash the Resin; 8.1. Overview; 8.2. Duration; References; Referenced Protocols in Methods Navigator; Chapter Four: Analysis of Protein-Protein Interactions by Coimmunoprecipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Isolation of the Protein of Interest by Immunoprecipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip.
  table of contents: 6. Step 2 Detection of the Binding Partner by Immunoblotting6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Section III: Protein Protocols/Protein Purification; Chapter Five: Use and Application of Hydrophobic Interaction Chromatography for Protein Purification; 1. Theory; 1.1. Latest technology in HIC adsorbents; 1.2. Advantages and disadvantages of using HIC; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 3.2. Preparation; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Column Equilibration.
  note: edited by Jon Lorsch.
  note: Includes bibliographical references and index.
  subject:
    topic: Proteins
    topic: Analysis
  subject:
    topic: Proteins
    topic: Purification
  subject:
    topic: Protéines
    topic: Analyse
  subject:
    topic: SCIENCE
    topic: Life Sciences
    topic: Biochemistry
  subject:
    topic: Proteins
    topic: Analysis
  subject:
    topic: Proteins
    topic: Purification
  classification: QP601
  classification: 572/.636
  classification: 612 23
  related item:
    title info:
      title: Laboratory Methods in Enzymology
    name:
      name part: Lorsch, Jon.
    origin info:
      publisher: Academic Press 2014
  related item:
    title info:
      title: Methods in enzymology ; volume 541
  identifier: 9780124201781
  identifier: 0124201784
  identifier: 1306540887
  identifier: 9781306540889
  identifier: 9780124201194
  identifier: 0124201199
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: NLGGC
    record creation date: 140603
    record change date: 20260326200924.1
    record identifier: in00005754533
    language of cataloging:
      language term: eng