Enzymes : biochemistry, biotechnology and clinical chemistry /

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering...

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Bibliographic Details
Main Authors: Palmer, Trevor, 1944- (Author), Bonner, Philip L. R. (Author)
Corporate Author: ScienceDirect (Online service)
Format: eBook
Language:English
Published: Oxford : Woodhead Publishing, [2011]
Edition:Second edition.
Subjects:
Online Access:Connect to the full text of this electronic book
Description
Summary:In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course. Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subjectDiscusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purificationIncludes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy.
Physical Description:1 online resource
Bibliography:Includes bibliographical references and index.
ISBN:9780857099921
0857099922