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Guide to molecular cloning techniques /

Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA...

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Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Berger, Shelby L. (Editor), Kimmel, Alan R. (Editor)
Format: eBook
Language:English
Language Notes:English.
Published: Orlando : Academic Press, 1987.
Series:Methods in enzymology ; v. 152.
Subjects:
Molecular cloning.
Molecular cloning > Technique.
Cloning > Technique.
DNA.
Recombinant DNA.
Cloning, Molecular > methods
DNA, Recombinant
Cloning, Molecular
DNA
Clonage moléculaire > Technique.
Clonage > Technique.
ADN.
Clonage moléculaire.
ADN recombinant.
42.15 cytology, cell biology and cell physiology.
Cloning.
Clonage.
ADN = Acide désoxyribonucléique.
Génétique.
Manuels.
Recombinant DNA
Molecular cloning > Technique
Molecular cloning
Genklonierung
Aufsatzsammlung
Klonierung
Nucleinsäuren
Methode
Kloneren.
Biologia molecular
Laboratorio
Acido nucleico
DNA recombinante
Bacteria
Clonagem
RNA
Molecular biology
Laboratory
Nucleic acids
Cloning
Clones
Electronic books.
Aufsatzsammlung.
Textbooks (form)
Online Access:Connect to the full text of this electronic book
Description
Summary:Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones.
Physical Description:1 online resource (xl, 812 pages) : illustrations
Format:Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Bibliography:Includes bibliographical references and index.
ISSN:0076-6879 ;