rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Molecular biology techniques
    sub title: a classroom laboratory manual
  name:
    name part: Carson, Sue (Susan)
    role:
      role term: creator
  name:
    name part: Miller, Heather B.
  name:
    name part: Witherow, D. Scott.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  genre: Handbook
  genre: Laboratory Manual
  genre: handbooks.
  genre: manuals (instructional materials)
  genre: Handbooks and manuals
  genre: Laboratory manuals
  genre: Handbooks and manuals.
  genre: Laboratory manuals.
  genre: Guides et manuels.
  genre: Manuels de laboratoire.
  origin info:
    place:
      place term: ne
    place:
      place term: Amsterdam
    place:
      place term: Boston
    publisher: Elsevier /Academic Press
    date issued: ©2012
    date issued: 2012
    edition: 3rd ed.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (xxvi, 200 pages) : color illustrations.
  abstract: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions.
  table of contents: Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions.
  note: Susan Carson, Heather Miller, D. Scott Witherow.
  note: Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006.
  note: Includes bibliographical references and index.
  note: English.
  subject:
    topic: Molecular biology
    genre: Laboratory manuals
  subject:
    topic: Molecular biology
    topic: Technique
    genre: Handbooks, manuals, etc
  subject:
    topic: Recombinant DNA
  subject:
    topic: Protein engineering
  subject:
    topic: DNA, Recombinant
  subject:
    topic: Molecular Biology
    topic: methods
  subject:
    topic: Protein Engineering
  subject:
    topic: Biologie moléculaire
    genre: Manuels de laboratoire
  subject:
    topic: Biologie moléculaire
    topic: Technique
    genre: Guides, manuels, etc
  subject:
    topic: ADN recombinant
  subject:
    topic: Technologie des protéines
  subject:
    topic: SCIENCE
    topic: Life Sciences
    topic: Molecular Biology
  subject:
    topic: Recombinant DNA
  subject:
    topic: Protein engineering
  subject:
    topic: Molecular biology
  subject:
    topic: Molecular biology
    topic: Technique
  subject:
    topic: Methode
  subject:
    topic: Molekularbiologie
  subject:
    topic: Methode
    topic: (DE-588c)4038971-6
  subject:
    topic: Molekularbiologie
    topic: (DE-588c)4039983-7
  classification: QH506 .C36 2012eb
  classification: 572.8/078
  classification: QU 25
  classification: WC 4150
  related item:
    title info:
      title: Molecular biology techniques
    name:
      name part: Carson, Sue (Susan).
    origin info:
      publisher: Oxford ; Waltham, MA : Academic Press, 2012
      edition: 3rd ed.
    identifier: (DLC) 2012359021
    identifier: (OCoLC)754167841
  related item:
    title info:
      title: ClinicalKey
  identifier: 9780123855459
  identifier: 0123855454
  identifier: 9780123855442
  identifier: 0123855446
  identifier: 9786613310972
  identifier: 6613310972
  identifier: 1104879:10110003 Elsevier Science & Technology
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: E7B
    record creation date: 111109
    record change date: 20260326190622.0
    record identifier: in00005748795
    language of cataloging:
      language term: eng