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Part B, Biochemical and analytical methods
1 online resource (xxx, 743 pages) : illustrations.
Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin is again central to a new area: development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion, Volume 232, will be indispensable to anyone with a serious interest in this emerging field. They completely update and extend the information presented in Volume 76 published a decade ago. Key Features * Preparation, purification, and analysis of human hemoglobin and of various non-human hemoglobins * Preparation of hemoglobin hybrids and of model heme compounds * Preparation of chemically modified hemoglobins and of recombinant hemoglobins * Analytical procedures presented for studying hemoglobin stability and degradation and for the formation of xenobiotic adducts of hemoglobin.
1.Pilot-Scale Preparation of Hemoglobin Solutions / Robert M. Winslow / Keith W. Chapman -- 2.Detection of Red Cell Membrane Components in Human Hemoglobin Preparations / Bernard J.-M. Thevenin / Stephen B. Shohet -- 3.Detection of Globin Chains by Reversed-Phase High-Performance Liquid Chromatography / Bruno Masala / Laura Manca -- 4.Structural Characterization of Hemoglobin Variants Using Capillary Electrophoresis and Fast Atom Bombardment Mass Spectrometry / Pasquale Ferranti / Antonio Malorni / Pietro Pucci -- 5.Purification of Glycated Hemoglobin / Margo Panush Cohen / Van-Yu Wu -- 6.Measurement of Endotoxin Levels in Hemoglobin Preparations / Robert I. Roth / Jack Levin -- 7.Preparation and Characterization of insect Hemoglobins from Chironomus thummi thummi / Hans Heinrich Ruf / Andreas G. Altemuller / Klaus Gersonde -- 8.Preparation and Characterization of Invertebrate Globin Complexes / Serge N. Vinogradov / Pawan K. Sharma -- 9.Fish Hemoglobins / Alice DeYoung / Laura D. Kwiatkowski / Robert W. Noble -- 10.Conformational and Functional Characteristics of Bovine Hemoglobin / Clara Fronticelli / Enrico Bucci -- 11.Synthetic Hemes / Eishun Tsuchida / Teruyuki Komatsu -- 12.Semisynthesis of Hemoglobin / Rajendra Prasad Roy / A. Seetharama Acharya -- 13.Preparation of Mixed Metal Hybrids / Antonio Tsuneshige / Takashi Yonetani -- 14.Preparation of Hemoglobin Derivatives Selectively or Randomly Modified at Amino Groups / James M. Manning -- 15.Amidation of Basic Carboxyl Groups of Hemoglobin / M. Janardhan Rao / A. Seetharama Acharya -- 16.Bis(pyridoxal) Polyphosphates as Specific Intramolecular Cross-Linking Agents for Hemoglobin / Ruth E. Benesch -- 17.Preparation of Intramolecularly Cross-Linked Hemoglobins / Roxanne Y. Walder / Mark E. Andracki / Joseph A. Walder -- 18.Derivatives of Hemoglobin Prepared by Reaction with Aryl or Alkyl Isothiocyanates / Douglas L. Currell -- 19.Hemoglobin Polymerization / Shirley L. MacDonald / Duncan S. Pepper -- 20.Preparation of Conjugated Hemoglobins / Hong Xue / J. Tze-Fei Wong -- 21.Structural Characterization of Modified Hemoglobins / Richard T. Jones -- 22.Production of Human Hemoglobin in Escherichia coli Using Cleavable Fusion Protein Expression Vector / Timm-H. Jessen / Noboru H. Komiyama / Jeremy Tame / Jose Pagnier / Daniel Shih / Ben Luisi / Giulio Fermi / Kiyoshi Nagai -- 23.Expression of Recombinant Human Hemoglobin in Escherichia coli / Douglas Looker / Antony J. Mathews / Justin O. Neway / Gary L. Stetler -- 24.Production of Recombinant Human Hemoglobin A in Saccharomyces cerevisiae / Jill E. Ogden / Roy Harris / Michael T. Wilson -- 25.Purification and Characterization of Recombinant Human Sickle Hemoglobin Expressed in Yeast / Jose Javier Martin De Llano / Olaf Schneewind / Gary L. Stetler / James M. Manning -- 26.Preparation of Recombinant Hemoglobin in Transgenic Mice / Michael P. Reilly / Steven L. McCune / Thomas M. Ryan / Tim M. Townes / Makoto Katsumata / Toshio Asakura -- 27.Transgenic Swine as Recombinant Production System for Human Hemoglobin / John S. Logan / Michael J. Martin -- 28.Detection, Formation, and Relevance of Hemichromes and Hemochromes / Joseph M. Rifkind / Omoefe Abugo / Abraham Levy / Jane Heim -- 29.Measuring Relative Rates of Hemoglobin Oxidation and Denaturation / Victor W. Macdonald -- 30.Hydrogen Peroxide-Mediated Ferrylhemoglobin Generation in Vitro and in Red Blood Cells / Cecilia Giulivi / Kelvin J. A. Davies -- 31.The Stability of the Heme-Globin Linkage: Measurement of Heme Exchange / Ruth E. Benesch -- 32.Release of iron from Hemoglobin / S. Scott Panter -- 33.Thermal Denaturation Procedures for Hemoglobin / Kenneth W. Olsen -- 34.Photochemical Reduction of Methemoglobin and Methemoglobin Derivatives / Johannes Everse -- 35.Regeneration of Functional Hemoglobin from Partially Oxidized Hemoglobin in the Presence of Molecular Hydrogen and a Multicomponent Redox Catalyst / Evelyn L. McGown / Mazhar Khan / Kilian Dill -- 36.Peroxidative Activities of Hemoglobin and Hemoglobin Derivatives / Johannes Everse / Maria C. Johnson / Mario A. Marini -- 37.Cyclooxygenase Activity of Hemoglobin / Lucilla Zilletti / Mario Ciuffi / Sergio Franchi-Micheli / Fabio Fusi / Grazia Gentilini / Gloriano Moneti / Massimo Valoti / Gian Pietro Sgaragli -- 38.Hydroxylation and Dealkylation Reactions Catalyzed by Hemoglobin / J. J. Mieyal / D. W. Starke -- 39.Oxidation of Olefins Catalyzed by Hemoglobin / Giorgio Belvedere / Michele Samaja -- 40.Quantitative Analysis of Hemoglobin-Xenobiotic Adducts / Steven R. Tannenbaum / Paul L. Skipper -- 41.Quantitative Mass Spectrometry of Hemoglobin Adducts / John S. Wishnok -- 42.Aromatic Amine-Hemoglobin Adducts / Paul L. Skipper / W. G. Stillwell -- 43.Epoxide Adducts to N-Terminal Valine of Hemoglobin / Margareta Tornqvist -- 44.Tobacco-Specific Nitrosamine-Hemoglobin Adducts / Stephen S. Hecht / Steven G. Carmella / Sharon E. Murphy -- 45.Benzo[a]pyrene Diol Epoxide and Related Polynuclear Aromatic Hydrocarbon Adducts of Hemoglobin / Koli Taghizadeh / Paul L. Skipper -- 46.Analysis of Hemoglobin Adducts by Fluorescence Spectroscopy / Billy W. Day / Kuldip Singh -- Addendum to Article [11] / Eishun Tsuchida / Teruyuki Komatsu.
edited by Johannes Everse, Kim D. Vandegriff, Robert M. Winslow.
Includes bibliographical references and indexes.
Hemoglobins. Part B, Biochemical and analytical methods
Methods in enzymology ; volume 231, pt. B