http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
1 online resource (xlii, 356 pages, 4 unnumbered pages of plates) : illustrations (some color)
Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over antiogenesis. Diseases that are angiogensis-dependent result when blood vessels either grow excessively or insufficiently. * Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition * Provides the "builder's manual" for essential techniques--a one-stop shop that eliminates needless searching among untested techniques * Includes step-by-step methods for understanding the cell and molecular basis of wound healing, vascular integrin signaling, mechanical signaling in blood vessels, and vascular proteomics
Cover; Copyright Page; TOCContents; Contributors; Preface; Volumes in Series; CHChapter 1: Approaches for Studying Angiogenesis-Related Signal Transduction; 1. Introduction; 2. Angiogenesis; 3. Phosphorylation of eNOS on Ser1179; 4. Measuring Released NO by No-Specific Chemiluminescence; 5. Concluding Remarks; References; CHChapter 2: Physiologic Stress-Mediated Signaling in the Endothelium; 1. Introduction; 2. Parallel Plate Flow System; 3. Cone and Plate Flow System; 4. Cyclic Stretching of the Endothelium; Acknowledgments; References.
CHChapter 3: Endothelial Cell Adhesion and Migration1. Introduction; 2. Cell Preparation; 3. Preparation of Well-Defined Surfaces; 4. Preparation of Polyacrylamide Substrates for Cell Adhesion Studies; 5. Quantifying Cell Adhesion; 6. Quantifying Endothelial Cell Migration; References; CHChapter 4: An Optimized Three-Dimensional In Vitro Model for the Analysis of Angiogenesis; 1. Overview; 2. Fibrin Bead Assay; 3. Immunocytochemistry of Angiogenic Sprouts In Vitro; 4. Retroviral Transduction of EC; 5. Laser Capture Microdissection; 6. Discussion; References.
CHChapter 5: In Vitro Three Dimensional Collagen Matrix Models of Endothelial Lumen Formation During Vasculogenesis and Angiogenesis1. Introduction; 2. In Vitro Models of Vasculogenesis and Angiogenesis in 3D Collagen Matrices; 3. Molecular/Genetic Manipulation of ECs; 4. Rho GTPase Activation Assay During EC Tube Morphogenesis in 3D Collagen Matrices; 5. Biochemical Analysis of Interactions Between Cdc42 and its Downstream Effectors During EC Lumen and Tube Formation in 3D Colla; 6. Conclusions; Acknowledgments; References.
CHChapter 6: In Vitro Differentiation of Mouse Embryonic Stem Cells Into Primitive Blood Vessels1. Introduction; 2. Maintenance of ES Cells; 3. Generation of Transgenic ES Cell Lines; 2. Concluding Remarks; Acknowledgments; References; CHChapter 7: The Aortic Ring Model of Angiogenesis; 1. Introduction; 2. Aortic Ring Angiogenesis Model: Basic Protocol; 3. Protocols for Analysis of Aortic Cultures; 4. Modifications of Basic Protocol; 5. Troubleshooting; 6. Concluding Remarks; References; CHChapter 8: An Assay System for In Vitro Detection of Permeability in Human ""Endothelium""
1. Introduction2. Generation of a Human Endothelium Model for Permeability Studies; 3. Conclusion; Acknowledgments; References; CHChapter 9: Assays of Transendothelial Migration In Vitro; 1. Introduction; 2. Methods for Investigating Leukocyte Transendothelial Migration Under Static or Flow Conditions In Vitro; Acknowledgments; References; CHChapter 10: Cell Mechanics at Multiple Scales; 1. Introduction; 2. Large-Scale Cell Stretching; 3. Magnetic Micromanipulation; 4. Fluorescence Laser Tracking Microrheology; 5. General Summary and Conclusion; Acknowledgments; References.
Includes bibliographical references and indexes.
Amsterdam ; San Diego : Elsevier/Academic Press, ©2008