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Methods in plant molecular biology /

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Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protei...

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Bibliographic Details
Main Author: Schuler, Mary A.
Corporate Author: ScienceDirect (Online service)
Other Authors: Zielinski, Raymond E.
Format: eBook
Language:English
Language Notes:English.
Published: San Diego : Academic Press, ©1989.
Subjects:
Plant molecular biology > Experiments.
Molecular Biology > methods
Molecular Biology > experiments.
Plants
Biologie moléculaire végétale > Expériences.
Plantes.
vegetation.
Plant molecular biology > Experiments
Pflanzen
Molekularbiologie
Praktikum
Methode
Plants > Chemistry > Laboratory manuals.
Plant genetics > Laboratory manuals.
Plant molecular biology > Laboratory manuals.
Botany, Experimental > Laboratory manuals.
Biologie moléculaire > Manuels de laboratoire.
Physiologie végétale.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Front Cover; Methods in Plant Molecular Biology; Copyright Page; Table of Contents; Preface; Laboratory Schedule; Chapter 1. Restriction Mapping of Plasmid DNA; Introduction; Protocols; Materials Provided; References; Chapter 2. Cloning of Restriction Fragments into pUC Vectors; Introduction; Protocols; Materials Provided; References; Chapter 3A. Preparation of Intact Chloroplasts from Pea; Introduction; Protocols; Materials Provided; References; Chapter 3B. Lowry Assay for Protein Determination; Introduction; Protocol; Materials Provided; Reference
  • Chapter 3C. Protein Synthesis by Isolated Pea ChloroplastsIntroduction; Special Precautions for Use of Radioisotopes; Protocol; Materials Provided; References; Chapter 3D. Separation of Thylakoid and Stromal Proteins by SDS-Gel Electrophoresis; Introduction; Protocols; Materials Provided; References; Chapter 4. Isolation of Chloroplast DNA; Introduction; Protocols; Materials Provided; References; Chapter 5A. RNA Isolation from Light- and Dark-Grown Seedlings; Introduction; Protocol; Materials Provided; References
  • Chapter 5B. Preparation of a Wheat Germ Extract for in Vitro Translation of mRNAIntroduction; Special Precautions for Use of Radioisotopes; Protocols; Materials Provided; References; Chapter 6A. Screening of Recombinant Phage Libraries with Cloned CDNA Probes; Introduction; Protocols; Materials Provided; References; Chapter 6B. Isolation of Phage DNA from Liquid Cultures; Introduction; Protocol; Materials Provided; Chapter 7. Dideoxy DNA Sequencing; Introduction; Protocols; Materials Provided; References; Chapter 8. Transformation of Leaf Discs with Agrobacterium; Introduction; Protocols
  • Materials ProvidedReferences; Appendix I: Glossary; Terms and Definitions; Abbreviations; Appendix II: Gel Electrophoresis Equipment; Power Supplies; Electrophoresis Rigs; Index