Methods in cell biology. Volume 40, A practical guide to the study of calcium in living cells /
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research...
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| Format: | eBook |
| Language: | English |
| Language Notes: | English. |
| Published: |
San Diego :
Academic Press,
©1994.
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| Series: | Methods in cell biology ;
v. 40. |
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
| Summary: | A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Key Features * Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes * Fluorescent dyes * Luminescent proteins * Includes techniques for perturbing intracellular Ca2+ * Covers detailed methodology plus problems and pitfalls of each technique * Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program * Color plates illustrate techniques such as * Confocal ratio-imaging * Use of aequorin. |
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| Physical Description: | 1 online resource (xiii, 368 pages) : illustrations. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: | 0125641419 9780125641418 1281514098 9781281514097 9786611514099 6611514090 9780125228107 0125228104 0080859380 9780080859385 |