http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
functional genomics, proteomics, and other systems analysis
1 online resource (lii, 892 pages) : illustrations
This fully updated edition of the best-selling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. Provides up-to-date methods necessary to study genes in yeast. Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field.
Section I. Functional genomics -- 1. Analysis of gene function using DNA microarrays / Andrew P. Capaldi -- 2. An introduction to microarray data analysis and visualization / Gregg B. Whitworth -- 3. Genome-wide approaches to monitor pre-mRNA splicing / Maki Inada and Jeffrey A. Pleiss -- 4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites / Philippe Lefrançois, Wei Zheng and Michael Snyder -- 5. Genome-wide mapping of nucleosomes in yeast / Oliver J. Rando -- 6. Genome-wide translational profiling by ribosome footprinting / Nicholas T. Ingolia -- Section II. Systematic genetic analysis -- 7. Synthetic genetic array (SGA) analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe / Anastasia Baryshnikova [and others] -- 8. Making temperature-sensitive mutants / Shay Ben-Aroya [and others] -- 9. Quantitative genetic interaction mapping using the E-MAP approach / Sean R. Collins, Assen Roguev and Nevan J. Krogan -- 10. Exploring gene function and drug action using chemogenomic dosage assays / Elke Ericson [and others] -- Section III. Proteomics -- 11. Yeast expression proteomics by high-resolution mass spectrometry / Tobias C. Walther, Jesper V. Olsen and Matthias Mann -- 12. High-quality binary interactome mapping / Matija Dreze [and others] -- 13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry-based proteomics / Bernd Bodenmiller and Ruedi Aebersold -- 14. A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells / Stephen W. Michnick [and others] -- 15. Yeast lipid analysis and quantification by mass spectrometry / Xue Li Guan [and others] -- 16. Mass spectrometry-based metabolomics of yeast / Christopher A. Crutchfield [and others] -- Section IV. Systems Analysis -- 17. Imaging single mRNA molecules in yeast / Hyun Youk, Arjun Raj and Alexander van Oudenaarden -- 18. Reconstructing gene histories in Ascomycota fungi / Ilan Wapinski and Aviv Regev -- 19. Experimental evolution in yeast: a practical guide / Maitreya J. Dunham -- 20. Enhancing stress resistance and production phenotypes through transcriptome engineering / Felix H. Lam [and others] -- Section V. Advances in Cytology/Biochemistry -- 21. Visualizing yeast chromosomes and nuclear architecture / Peter Meister [and others] -- 22. Quantitative localization of chromosomal loci by immunofluorescence / Donna Garvey Brickner, William Light and Jason H. Brickner -- 23. Spinning disk confocal microscopy of yeast / Kurt Thorn -- 24. Correlative GFP-immunoelectron microscopy in yeast / Christopher Buser and Kent McDonald -- 25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae / J. Ross Buchan, Tracy Nissan and Roy Parker -- 26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization / Daniel Zenklusen and Robert H Singer -- 27. The Use of in vitro assays to measure endoplasmic reticulum-associated degradation (ERAD) / Jeffrey L. Brodsky -- 28. A protein transformation protocol for introducing yeast prion particles into yeast / Motomasa Tanaka -- 29. Overexpression and purification of integral membrane proteins in yeast / Franklin A. Hays, Zygy Roe-Zurz, and Robert M. Stroud -- 30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast / Simon Alberti, Randal Halfmann and Susan Lindquist -- Section VI. Other fungi -- 31. Genetics and molecular biology in Candida albicans / Aaron D. Hernday [and others] -- 32. Molecular genetics of Schizosaccharomyces pombe / Sarah A. Sabatinos and Susan L. Forsburg -- 33. Applying genetics and molecular biology to the study of the human pathogen Cryptococcus neoformans / Cheryl D. Chun and Hiten D. Madhani -- 34. The Fungal Genome Initiative and lessons learned from genome sequencing / Christina A. Cuomo and Bruce W. Birren -- 35. Ultradian metabolic cycles in yeast / Benjamin P. Tu.
edited by Jonathan Weissman, Christine Guthrie, Gerald R. Fink.
Title from PDF title page (ScienceDirect, viewed March 3, 2010).
Includes bibliographical references and index.